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View article: Study of the Role of the Tumor Microenvironment in Ovarian Cancer (<scp>MICO</scp>): A Prospective Monocentric Trial
Study of the Role of the Tumor Microenvironment in Ovarian Cancer (<span>MICO</span>): A Prospective Monocentric Trial Open
Background Ovarian cancer (OC) is one of the most aggressive tumors requiring new therapeutic approaches. Immunotherapy represents an opportunity, but to date, OC patients do not appear to benefit from current protocols. A better understan…
View article: Investigating the hypoglycaemic potential of processed apple and acarbose combination <i>in vitro</i> , <i>ex vivo</i> , and <i>in vivo</i> : the role of quercetin-3-glucoside in steering α-glucosidase inhibition
Investigating the hypoglycaemic potential of processed apple and acarbose combination <i>in vitro</i> , <i>ex vivo</i> , and <i>in vivo</i> : the role of quercetin-3-glucoside in steering α-glucosidase inhibition Open
This study investigated the interaction between apple juice (AJ) and acarbose (A) in modulating glycemic responses, with the aim of validating in vivo results previously observed in vitro .
View article: Update on the Biological and Clinical Relevance of Mast Cells in Chronic Rhinosinusitis with Nasal Polyps
Update on the Biological and Clinical Relevance of Mast Cells in Chronic Rhinosinusitis with Nasal Polyps Open
Chronic rhinosinusitis with nasal polyps (CRSwNP) is a common inflammatory disorder whose complex immunopathogenesis has yet to be fully elucidated. Endotype-2 CRSwNP is the most common form of disease where eosinophils are the main driver…
View article: Supplementary Figure S7 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Figure S7 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary Figure S7. Flow cytometry characterization of TNFa receptors and TNFa production. A. List of cytokines and chemokines tested through a multiplex immunoassay (Procarta-plex by Thermofisher) in the supernatants of ST4787 or T23…
View article: Supplementary Figure S5 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Figure S5 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary Figure S5. Expression of OPN in MC/9 cells and effect on T23 cell proliferation. A. Separate channels of immunofluorescence for DAPI (cyan), OPN (red) and WGA (blue) of MC/9, MC/9-CTR, MC/9-OPNf, and MC/9-iOPN cells showed in…
View article: Data from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Data from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Neuroendocrine prostate cancer (NEPC) is an aggressive form of prostate cancer that emerges as tumors become resistant to hormone therapies or, rarely, arises de novo in treatment-naïve patients. The urgent need for effective therapies aga…
View article: Supplementary Figure S4 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Figure S4 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary Figure S4. Characterization of additional cell lines. A. Immunofluorescence for DAPI (blue) and CGA (red) on murine NEPC cells (TC566 and TC411K). B. Immunofluorescence for DAPI (blue) and OPN (red) on HMC-1 cells. C. Western…
View article: Supplementary Figure S2 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Figure S2 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary Figure S2. OPN evaluation in bone marrow-derived MCs. A. Separate channels of immunofluorescence for DAPI (cyan), OPN (red) and WGA (blue) of WT and OPN-/- MCs showed in Fig. 1A B. Representative images of 3 different biologi…
View article: Supplementary Figure S9 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Figure S9 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary Figure S9. Expression and silencing of putative TLR ligands in NEPC cells. A. Venn diagrams show a list of 29 genes (List 3; Supplementary Table S3) extrapolated from the intersection between genes found up-regulated in TRAMP…
View article: Supplementary Figure S2 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Figure S2 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary Figure S2. OPN evaluation in bone marrow-derived MCs. A. Separate channels of immunofluorescence for DAPI (cyan), OPN (red) and WGA (blue) of WT and OPN-/- MCs showed in Fig. 1A B. Representative images of 3 different biologi…
View article: Supplementary Figure S1 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Figure S1 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary Figure S1. Flow cytometry evaluation of murine MC purity and maturation in vitro. A. Gating strategy used to evaluate the purity and maturation of WT, OPN-/-, MyD88-/- and TNFa-/-MCs by flow cytometry. Mature MC population is…
View article: Supplementary Figure S8 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Figure S8 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary Figure S8. Hematoxylin and eosin staining in TRAMP mice. A. Hematoxylin and eosin staining in serial slides of tumors reported in Fig. 5A, showing an untreated TRAMP mouse with adenocarcinoma (ADENO) and a TRAMP mouse subject…
View article: Supplementary Table 2 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Table 2 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary table 2 - list of siRNA and Taqman assays used in the study
View article: Supplementary Figure S9 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Figure S9 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary Figure S9. Expression and silencing of putative TLR ligands in NEPC cells. A. Venn diagrams show a list of 29 genes (List 3; Supplementary Table S3) extrapolated from the intersection between genes found up-regulated in TRAMP…
View article: Supplementary Figure S7 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Figure S7 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary Figure S7. Flow cytometry characterization of TNFa receptors and TNFa production. A. List of cytokines and chemokines tested through a multiplex immunoassay (Procarta-plex by Thermofisher) in the supernatants of ST4787 or T23…
View article: Supplementary Table 4 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Table 4 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary table 4 - transcript levels of the 29 TLR ligands measured by high throughput customized Taqman assay
View article: Supplementary Table 1 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Table 1 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary table 1 - list of antibodies used in the study
View article: Supplementary Table 1 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Table 1 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary table 1 - list of antibodies used in the study
View article: Supplementary Table 3 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Table 3 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary table 3 - List of putative NEPC-specific surface TLR ligands
View article: Supplementary Figure S8 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Figure S8 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary Figure S8. Hematoxylin and eosin staining in TRAMP mice. A. Hematoxylin and eosin staining in serial slides of tumors reported in Fig. 5A, showing an untreated TRAMP mouse with adenocarcinoma (ADENO) and a TRAMP mouse subject…
View article: Supplementary Figure S3 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Figure S3 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary Figure S3. Toluidine blue for detection of MCs in mice. A. MC count reported as number of MCs/tumor area (cm2) in tumor samples from TRAMP mice with prostate intraepithelial neoplasia or adenocarcinoma (PIN/ADENO, n= 12), or …
View article: Supplementary Figure S6 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Figure S6 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary Figure S6. Quantification of western blot. A. Quantification of western blot reported in Fig. 3G. Western blots were validated twice. All histograms depict mean ± SD of biological replicates (represented by dots). One-way ANO…
View article: Supplementary Figure S5 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Figure S5 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary Figure S5. Expression of OPN in MC/9 cells and effect on T23 cell proliferation. A. Separate channels of immunofluorescence for DAPI (cyan), OPN (red) and WGA (blue) of MC/9, MC/9-CTR, MC/9-OPNf, and MC/9-iOPN cells showed in…
View article: Data from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Data from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Neuroendocrine prostate cancer (NEPC) is an aggressive form of prostate cancer that emerges as tumors become resistant to hormone therapies or, rarely, arises de novo in treatment-naïve patients. The urgent need for effective therapies aga…
View article: Supplementary Figure S4 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Figure S4 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary Figure S4. Characterization of additional cell lines. A. Immunofluorescence for DAPI (blue) and CGA (red) on murine NEPC cells (TC566 and TC411K). B. Immunofluorescence for DAPI (blue) and OPN (red) on HMC-1 cells. C. Western…
View article: Supplementary Figure S6 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Figure S6 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary Figure S6. Quantification of western blot. A. Quantification of western blot reported in Fig. 3G. Western blots were validated twice. All histograms depict mean ± SD of biological replicates (represented by dots). One-way ANO…
View article: Supplementary Figure S1 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Figure S1 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary Figure S1. Flow cytometry evaluation of murine MC purity and maturation in vitro. A. Gating strategy used to evaluate the purity and maturation of WT, OPN-/-, MyD88-/- and TNFa-/-MCs by flow cytometry. Mature MC population is…
View article: Supplementary Table 2 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer
Supplementary Table 2 from Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate Cancer Open
Supplementary table 2 - list of siRNA and Taqman assays used in the study