Casper Berger
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View article: Xenon plasma focused ion beam lamella fabrication on high-pressure frozen specimens for structural cell biology
Xenon plasma focused ion beam lamella fabrication on high-pressure frozen specimens for structural cell biology Open
Cryo focused ion beam lamella preparation is a potent tool for in situ structural biology, enabling the study of macromolecules in their native cellular environments. However, throughput is currently limited, especially for thicker, more b…
View article: Xenon plasma focused ion beam lamella fabrication on high-pressure frozen specimens for structural cell biology
Xenon plasma focused ion beam lamella fabrication on high-pressure frozen specimens for structural cell biology Open
Cryo focused ion beam lamella preparation is a potent tool for in situ structural biology, enabling the study of macromolecules in their native cellular environments. However, throughput is currently limited, especially for thicker, more b…
View article: RedLionfish – fast Richardson-Lucy Deconvolution package for efficient point spread function suppression in volumetric data
RedLionfish – fast Richardson-Lucy Deconvolution package for efficient point spread function suppression in volumetric data Open
The experimental limitations with optics observed in many microscopy and astronomy instruments result in detrimental effects for the imaging of objects. This can be generally described mathematically as a convolution of the real object ima…
View article: Cryo-electron tomography on focused ion beam lamellae transforms structural cell biology
Cryo-electron tomography on focused ion beam lamellae transforms structural cell biology Open
Cryogenic electron microscopy and data processing enable the determination of structures of isolated macromolecules to near-atomic resolution. However, these data do not provide structural information in the cellular environment where macr…
View article: Plasma FIB milling for the determination of structures <i>in situ</i>
Plasma FIB milling for the determination of structures <i>in situ</i> Open
Structural biology inside cells and tissues requires methods able to thin vitrified specimens to electron transparent thicknesses. Until now, focused ions beams based on gallium have been used. However, ion implantation, changes to surface…
View article: Structure of the Yersinia injectisome in intracellular host cell phagosomes revealed by cryo FIB electron tomography
Structure of the Yersinia injectisome in intracellular host cell phagosomes revealed by cryo FIB electron tomography Open
Many pathogenic bacteria use the type III secretion system (T3SS), or injectisome, to secrete toxins into host cells. These protruding systems are primary targets for drug and vaccine development. Upon contact between injectisomes and host…
View article: Mycobacterial protein weaponry studied by cryo‐correlative microscopy
Mycobacterial protein weaponry studied by cryo‐correlative microscopy Open
In correlative light and electron microscopy, the discerning power of fluorescence microscopy (FM) is combined with the ultra‐high resolution of electron microscopy (EM). We fluorescently label protein complexes in cells, with the purpose …