Catherine A. Royer
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View article: AI-Driven Segmentation and Analysis of Microbial Cells
AI-Driven Segmentation and Analysis of Microbial Cells Open
Studying the growth and metabolism of microbes provides critical insights into their evolutionary adaptations to harsh environments, which are essential for microbial research and biotechnology applications. In this study, we developed an …
View article: Putting a lid on it: The N-terminal helix of Arf1 inhibits switching via uniform stabilization of the native state
Putting a lid on it: The N-terminal helix of Arf1 inhibits switching via uniform stabilization of the native state Open
The Arf (and Arf-like) GTPases, unlike all other Ras family GTPase members, exhibit a repressed conformation in the inactive, GDP-bound form. The N-terminal helix of Arf GTPases, which is missing in the other Ras family members, caps the s…
View article: Sequence determinants of allosteric back-to-front control of the Arf nucleotide switch
Sequence determinants of allosteric back-to-front control of the Arf nucleotide switch Open
Unlike AI-based protein structure prediction, the sequence determinants of protein dynamics, and thus function, remain elusive. The nucleotide switch in Arf GTPases involves a massive structural change, which we showed recently in Arf1 is …
View article: Swi4-dependent SWI4 transcription couples cell size to cell cycle commitment
Swi4-dependent SWI4 transcription couples cell size to cell cycle commitment Open
Growth-dependent accumulation of the G1/S transcription factor SBF, composed of Swi4 and Swi6, occurs in G1 phase in budding yeast and is limiting for commitment to division, termed Start. Here, we investigate the mechanisms for the size d…
View article: Response and adaptation of the transcriptional heat shock response to pressure
Response and adaptation of the transcriptional heat shock response to pressure Open
Introduction The molecular mechanisms underlying pressure adaptation remain largely unexplored, despite their significance for understanding biological adaptation and improving sterilization methods in the food and beverage industry. The h…
View article: A molten globule ensemble primes Arf1–GDP for the nucleotide switch
A molten globule ensemble primes Arf1–GDP for the nucleotide switch Open
The adenosine di-phosphate (ADP) ribosylation factor (Arf) small guanosine tri-phosphate (GTP)ases function as molecular switches to activate signaling cascades that control membrane organization in eukaryotic cells. In Arf1, the GDP/GTP s…
View article: Real-world evaluation of access-driven Canadian treatment sequences in progressive prostate cancer (REACTIVATE).
Real-world evaluation of access-driven Canadian treatment sequences in progressive prostate cancer (REACTIVATE). Open
Significant heterogeneity exists in the management and outcomes of mCRPC between provinces, particularly regarding the placement of Ra-223 in the treatment sequence.
View article: Phospholipid Scramblase 1 Controls Efficient Neurotransmission and Synaptic Vesicle Retrieval at Cerebellar Synapses
Phospholipid Scramblase 1 Controls Efficient Neurotransmission and Synaptic Vesicle Retrieval at Cerebellar Synapses Open
Phospholipids (PLs) are asymmetrically distributed at the plasma membrane. This asymmetric lipid distribution is transiently altered during calcium-regulated exocytosis, but the impact of this transient remodeling on presynaptic function i…
View article: Real-world evaluation of access-driven Canadian treatment sequences in progressive prostate cancer (REACTIVATE)
Real-world evaluation of access-driven Canadian treatment sequences in progressive prostate cancer (REACTIVATE) Open
Introduction: The results of the phase 3 ALSYMPCA trial showed that Radium-223 (Ra-223) improves overall survival (OS) and delays onset of first symptomatic skeletal event vs. placebo in patients with metastatic castration-resistant prosta…
View article: Swi4-dependent SWI4 transcription couples cell size to cell cycle commitment
Swi4-dependent SWI4 transcription couples cell size to cell cycle commitment Open
Summary Growth-dependent accumulation of the limiting SBF transcription factor, composed of Swi4 and Swi6, occurs in G1 phase in budding yeast and is limiting for commitment to division, termed Start. Here we measure size-dependence of Swi…
View article: Efficacy, tolerability, and endometrial safety of ospemifene compared with current therapies for the treatment of vulvovaginal atrophy: a systematic literature review and network meta-analysis
Efficacy, tolerability, and endometrial safety of ospemifene compared with current therapies for the treatment of vulvovaginal atrophy: a systematic literature review and network meta-analysis Open
Importance Ospemifene is a novel selective estrogen receptor modulator developed for the treatment of moderate to severe postmenopausal vulvovaginal atrophy (VVA). Objective The aim of the study is to perform a systematic literature review…
View article: Pressure pushes tRNA <sup>Lys3</sup> into excited conformational states
Pressure pushes tRNA <sup>Lys3</sup> into excited conformational states Open
Conformational dynamics play essential roles in RNA function. However, detailed structural characterization of excited states of RNA remains challenging. Here, we apply high hydrostatic pressure (HP) to populate excited conformational stat…
View article: Protocol for production and purification of SARS-CoV-2 3CLpro
Protocol for production and purification of SARS-CoV-2 3CLpro Open
3CLpro protease from SARS-CoV-2 is a primary target for COVID-19 antiviral drug development. Here, we present a protocol for 3CLpro production in Escherichia coli. We describe steps to purify 3CLpro, expressed as a fusion with the Saccharo…
View article: High Pressure CPMG and CEST Reveal That Cavity Position Dictates Distinct Dynamic Disorder in the PP32 Repeat Protein
High Pressure CPMG and CEST Reveal That Cavity Position Dictates Distinct Dynamic Disorder in the PP32 Repeat Protein Open
Given the central role of conformational dynamics in protein function, it is essential to characterize the time scales and structures associated with these transitions. High pressure (HP) perturbation favors transitions to excited states b…
View article: The microprotein Nrs1 rewires the G1/S transcriptional machinery during nitrogen limitation in budding yeast
The microprotein Nrs1 rewires the G1/S transcriptional machinery during nitrogen limitation in budding yeast Open
Commitment to cell division at the end of G1 phase, termed Start in the budding yeast Saccharomyces cerevisiae , is strongly influenced by nutrient availability. To identify new dominant activators of Start that might operate under differe…
View article: Structural evolution of the ancient enzyme, dissimilatory sulfite reductase
Structural evolution of the ancient enzyme, dissimilatory sulfite reductase Open
Dissimilatory sulfite reductase is an ancient enzyme that has linked the global sulfur and carbon biogeochemical cycles since at least 3.47 Gya. While much has been learned about the phylogenetic distribution and diversity of DsrAB across …
View article: Structural Evolution of the Ancient Enzyme, Dissimilatory Sulfite Reductase
Structural Evolution of the Ancient Enzyme, Dissimilatory Sulfite Reductase Open
Dissimilatory sulfite reductase is an ancient enzyme that has linked the global sulfur and carbon biogeochemical cycles since at least 3.47 Gya. While much has been learned about the phylogenetic distribution and diversity of DsrAB across …
View article: Structural Evolution of the Ancient Enzyme, Dissimilatory Sulfite Reductase
Structural Evolution of the Ancient Enzyme, Dissimilatory Sulfite Reductase Open
Dissimilatory sulfite reductase is an ancient enzyme that has linked the global sulfur and carbon biogeochemical cycles since at least 3.47 Gya. While much has been learned about the phylogenetic distribution and diversity of DsrAB across …
View article: Biological Networks across Scales—The Theoretical and Empirical Foundations for Time-Varying Complex Networks that Connect Structure and Function across Levels of Biological Organization
Biological Networks across Scales—The Theoretical and Empirical Foundations for Time-Varying Complex Networks that Connect Structure and Function across Levels of Biological Organization Open
Many biological systems across scales of size and complexity exhibit a time-varying complex network structure that emerges and self-organizes as a result of interactions with the environment. Network interactions optimize some intrinsic co…