Chad Sanada
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View article: Microfluidic System-based Time-course Tracking of Physical Proximity Between Cells and Its Effect on Gene Expression for Elucidating Live Single Cancer-immune Cell Interactions
Microfluidic System-based Time-course Tracking of Physical Proximity Between Cells and Its Effect on Gene Expression for Elucidating Live Single Cancer-immune Cell Interactions Open
Cell-cell communication and physical interactions play a vital role in cancer initiation, homeostasis, progression, and immune response. Here, we report a system that combines live capture of different cell types, co-incubation, time-lapse…
View article: Microfluidic system-based time-course tracking of physical proximity between cells and its effect on gene expression for elucidating live single cancer-immune cell interactions
Microfluidic system-based time-course tracking of physical proximity between cells and its effect on gene expression for elucidating live single cancer-immune cell interactions Open
Summary Cell-cell communication and physical interactions play a vital role in cancer initiation, homeostasis, progression, and immune response. Here, we report a system that combines live capture of different cell types, co-incubation, ti…
View article: A framework for evaluating edited cell libraries created by massively parallel genome engineering
A framework for evaluating edited cell libraries created by massively parallel genome engineering Open
Genome engineering methodologies are transforming biological research and discovery. Approaches based on CRISPR technology have been broadly adopted and there is growing interest in the generation of massively parallel edited cell librarie…
View article: Modeling expression ranks for noise-tolerant differential expression analysis of scRNA-seq data
Modeling expression ranks for noise-tolerant differential expression analysis of scRNA-seq data Open
Systematic delineation of complex biological systems is an ever-challenging and resource-intensive process. Single-cell transcriptomics allows us to study cell-to-cell variability in complex tissues at an unprecedented resolution. Accurate…
View article: Abstract LB-326: Full-Length mRNA transcriptome analysis of matched circulating tumor and immune cells from breast cancer subjects
Abstract LB-326: Full-Length mRNA transcriptome analysis of matched circulating tumor and immune cells from breast cancer subjects Open
Background Understanding the genetic heterogeneity of circulating immune and tumors cells at single-cell resolution will enable one to decipher the interplay between cancer and immune cells, and ultimately lead to better design of immunoth…
View article: Benchmarking Single-Cell RNA Sequencing Protocols for Cell Atlas Projects
Benchmarking Single-Cell RNA Sequencing Protocols for Cell Atlas Projects Open
Single-cell RNA sequencing (scRNA-seq) is the leading technique for charting the molecular properties of individual cells. The latest methods are scalable to thousands of cells, enabling in-depth characterization of sample composition with…
View article: The Molecular Signature of Megakaryocyte-Erythroid Progenitors Reveals a Role for the Cell Cycle in Fate Specification
The Molecular Signature of Megakaryocyte-Erythroid Progenitors Reveals a Role for the Cell Cycle in Fate Specification Open
(Cell Reports 25, 2083–2093.e1–e4; November 20, 2018) In the originally published version of this article, Chad Sanada was omitted from the author list. His affiliation is Department of Laboratory Medicine and Yale Stem Cell Center, Yale S…
View article: Corrigendum: Fluidic Logic Used in a Systems Approach to Enable Integrated Single-Cell Functional Analysis
Corrigendum: Fluidic Logic Used in a Systems Approach to Enable Integrated Single-Cell Functional Analysis Open
[This corrects the article on p. 70 in vol. 4, PMID: 27709111.].
View article: Fluidic Logic Used in a Systems Approach to Enable Integrated Single-Cell Functional Analysis
Fluidic Logic Used in a Systems Approach to Enable Integrated Single-Cell Functional Analysis Open
The study of single cells has evolved over the past several years to include expression and genomic analysis of an increasing number of single cells. Several studies have demonstrated wide spread variation and heterogeneity within cell pop…
View article: Adult human megakaryocyte-erythroid progenitors are in the CD34+CD38mid fraction
Adult human megakaryocyte-erythroid progenitors are in the CD34+CD38mid fraction Open
Key Points Purification strategies developed for human Mk-E progenitors, as well as committed Mk and E progenitors. MYB regulates the biphenotypic fate decision of human MEPs.
View article: Regulation of actin polymerization by tropomodulin-3 controls megakaryocyte actin organization and platelet biogenesis
Regulation of actin polymerization by tropomodulin-3 controls megakaryocyte actin organization and platelet biogenesis Open
Key Points Tmod3-null embryos have macrothrombocytopenia due to impaired MK cytoplasmic morphogenesis with defective proplatelet formation. F-actin polymerization and organization are disrupted in Tmod3-null MKs and in their proplatelet bu…