Chambers C. Hughes
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View article: Native metabolomics identifies pteridines as CutA ligands and modulators of copper binding
Native metabolomics identifies pteridines as CutA ligands and modulators of copper binding Open
CutA, a conserved protein across all domains of life, has been suggested to be involved in copper tolerance in bacteria, though recent studies have questioned this association, leaving its biological role unknown. To clarify its function, …
View article: Discovery and Heterologous Expression of the Soil Metagenome-Derived Lasso Peptide Metanodin with an Unprecedented Ring Structure
Discovery and Heterologous Expression of the Soil Metagenome-Derived Lasso Peptide Metanodin with an Unprecedented Ring Structure Open
Culture-independent metagenomic approaches have proven to be effective tools for identifying previously hidden biosynthetic gene clusters (BGCs) encoding novel natural products with potential medical relevance. However, producing these com…
View article: Identification of the negamycin split biosynthetic gene cluster in <i>Kitasatospora purpeofusca</i> ATCC21470
Identification of the negamycin split biosynthetic gene cluster in <i>Kitasatospora purpeofusca</i> ATCC21470 Open
SUMMARY Negamycin is a ribosome-targeting antibiotic with activity against Gram-positive and Gram-negative bacteria including ESKAPE pathogens. Furthermore, it promotes premature stop codon readthrough. Its therapeutic potential is limited…
View article: Enhancing tandem mass spectrometry-based metabolite annotation with online chemical labeling
Enhancing tandem mass spectrometry-based metabolite annotation with online chemical labeling Open
View article: Genome Mining Leads to the Discovery of Kasichelins A–D, Unusual β-Alanine- and β-Aminoisobutyric Acid-Containing Siderophores from <i>Streptomyces</i>
Genome Mining Leads to the Discovery of Kasichelins A–D, Unusual β-Alanine- and β-Aminoisobutyric Acid-Containing Siderophores from <i>Streptomyces</i> Open
Genome mining of a Streptomyces strain collected in Tanzania revealed its potential to produce a novel siderophore. Heterologous expression of the corresponding biosynthetic gene cluster (BGC) in S. coelicolor M512 resulted i…
View article: Application of a replicative targetable vector system for difficult-to-manipulate streptomycetes
Application of a replicative targetable vector system for difficult-to-manipulate streptomycetes Open
View article: Designer tetrazine probes for chemical labeling of isocyanide-containing natural products
Designer tetrazine probes for chemical labeling of isocyanide-containing natural products Open
The identification of novel antibacterial natural products (NPs) is paramount in confronting the global threat of antimicrobial resistance. New NPs with novel core structures and mechanisms of action (MoA) have become significantly more di…
View article: Native metabolomics identifies pteridines as CutA ligands and modulators of copper binding
Native metabolomics identifies pteridines as CutA ligands and modulators of copper binding Open
CutA, a conserved protein across all domains of life, has long been linked to copper tolerance in Escherichia coli , though recent studies question this association. To clarify its function, we studied cutA knockout mutants from two phylog…
View article: Strong pairwise interactions do not drive interactions in a plant leaf associated microbial community
Strong pairwise interactions do not drive interactions in a plant leaf associated microbial community Open
Microbial communities that promote plant growth show promise in reducing the impacts of climate change on plant health and productivity. Understanding microbe–microbe interactions in a community context is paramount for designing effective…
View article: Strong pairwise interactions do not drive interactions in a plant leaf associated microbial community
Strong pairwise interactions do not drive interactions in a plant leaf associated microbial community Open
Microbial communities that promote plant growth show promise in reducing the impacts of climate change on plant health and productivity. Understanding microbe-microbe interactions in a community context is paramount for designing effective…
View article: Antibacterial Marinopyrroles and Pseudilins Act as Protonophores
Antibacterial Marinopyrroles and Pseudilins Act as Protonophores Open
Elucidating the mechanism of action (MoA) of antibacterial natural products is crucial to evaluating their potential as novel antibiotics. Marinopyrroles, pentachloropseudilin, and pentabromopseudilin are densely halogenated, hybrid pyrrol…
View article: Challenging old microbiological treasures for natural compound biosynthesis capacity
Challenging old microbiological treasures for natural compound biosynthesis capacity Open
Strain collections are a treasure chest of numerous valuable and taxonomically validated bioresources. The Leibniz Institute DSMZ is one of the largest and most diverse microbial strain collections worldwide, with a long tradition of actin…
View article: Untargeted_Analysis_Cysteine
Untargeted_Analysis_Cysteine Open
Natural extracts treated with L-cysteine.
View article: Untargeted_Analysis_Hydroxylamine
Untargeted_Analysis_Hydroxylamine Open
LC-MS/MS DDA in positive mode of Natural extracts treated with hydroxylamine and untreated (Q exactive HF)
View article: Untargeted_Analysis_Cysteine
Untargeted_Analysis_Cysteine Open
Natural extracts treated with L-cysteine.
View article: Untargeted_Analysis_AQC
Untargeted_Analysis_AQC Open
LC-MS/MS DDA in positive mode of Natural extracts treated with AQC and untreated (Q exactive HF)
View article: Untargeted_Analysis_AQC
Untargeted_Analysis_AQC Open
LC-MS/MS DDA in positive mode of Natural extracts treated with AQC and untreated (Q exactive HF)
View article: Untargeted_Analysis_Hydroxylamine
Untargeted_Analysis_Hydroxylamine Open
LC-MS/MS DDA in positive mode of Natural extracts treated with hydroxylamine and untreated (Q exactive HF)
View article: 32 mix_Not treated
32 mix_Not treated Open
DDA in positive mode of a Mixture of 32 standard molecules analyzed on Impact II.
View article: 32 mix_Not treated
32 mix_Not treated Open
DDA in positive mode of a Mixture of 32 standard molecules analyzed on Impact II.
View article: Aktuelle Methoden in der antibakteriellen Naturstoffforschung
Aktuelle Methoden in der antibakteriellen Naturstoffforschung Open
Antibacterial natural products with novel chemical structures and unprecedented mechanisms of action inspire antibiotic drug discovery and form valuable tools for studying bacterial physiology. New technologies are being developed and impr…
View article: Antibacterial marinopyrroles and pseudilins act as protonophores
Antibacterial marinopyrroles and pseudilins act as protonophores Open
Elucidating the mechanism of action (MoA) of antibacterial natural products is crucial to evaluating their potential as novel antibiotics. The marinopyrroles, pentachloropseudilin, and pentabromopseudilin are densely halogenated, hybrid py…
View article: Evaluation of Data-Dependent MS/MS Acquisition Parameters for Non-Targeted Metabolomics and Molecular Networking of Environmental Samples: Focus on the Q Exactive Platform
Evaluation of Data-Dependent MS/MS Acquisition Parameters for Non-Targeted Metabolomics and Molecular Networking of Environmental Samples: Focus on the Q Exactive Platform Open
Non-targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a widely used tool for metabolomics analysis, enabling the detection and annotation of small molecules in complex environmental samples. Data-dependent acquisition (…
View article: Evaluation of Data Dependent MS/MS Acquisition Parameters for Non-targeted Metabolomics and Molecular Networking of Environmental Samples - Focus on the Q Exactive Platform
Evaluation of Data Dependent MS/MS Acquisition Parameters for Non-targeted Metabolomics and Molecular Networking of Environmental Samples - Focus on the Q Exactive Platform Open
Non-targeted liquid chromatography tandem mass spectrometry (LC-MS/MS) is a widely used tool for the detection and annotation of small molecules in complex environmental samples. Data Dependent Acquisition (DDA) of product ion spectra is t…
View article: The Cyanobacterial “Nutraceutical” Phycocyanobilin Inhibits Cysteine Protease Legumain
The Cyanobacterial “Nutraceutical” Phycocyanobilin Inhibits Cysteine Protease Legumain Open
The blue biliprotein phycocyanin, produced by photo‐autotrophic cyanobacteria including spirulina ( Arthrospira ) and marketed as a natural food supplement or “nutraceutical,” is reported to have anti‐inflammatory, antioxidant, immunomodul…
View article: Native metabolomics identifies the rivulariapeptolide family of protease inhibitors
Native metabolomics identifies the rivulariapeptolide family of protease inhibitors Open
View article: Biosynthesis and antifungal activity of fungus-induced <i>O</i>-methylated flavonoids in maize
Biosynthesis and antifungal activity of fungus-induced <i>O</i>-methylated flavonoids in maize Open
Fungal infection of grasses, including rice (Oryza sativa), sorghum (Sorghum bicolor), and barley (Hordeum vulgare), induces the formation and accumulation of flavonoid phytoalexins. In maize (Zea mays), however, investigators have emphasi…
View article: Specialized Metabolite-Mediated Predation Defense in the Marine Actinobacterium<i>Salinispora</i>
Specialized Metabolite-Mediated Predation Defense in the Marine Actinobacterium<i>Salinispora</i> Open
Bacteria inhabiting marine sediments are subject to predation by bacterivorous eukaryotes. Here, we test the hypothesis that sediment-derived bacteria in the genus Salinispora produce biologically active natural products that function as a…
View article: Native Metabolomics Identifies the Rivulariapeptolide Family of Protease Inhibitors
Native Metabolomics Identifies the Rivulariapeptolide Family of Protease Inhibitors Open
The identity and biological activity of most metabolites still remain unknown. A key bottleneck in the full exploration of this tremendous source of new structures and pharmaceutical activities is the compound purification needed for bioac…
View article: CCDC 1888527: Experimental Crystal Structure Determination
CCDC 1888527: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …