Explanipedia

“Late endosome transport by RILP-RAB7A promotes dendrite arborization.” Open

Chan Choo Yap, Laura Digilio, Lloyd P McMahon, Ryan J. Mulligan, Isabelle F. Witteveen , et al. · 2025

Vesicular transport along dendrites is well documented. Yet, the need for and regulation of directional dendritic transport is less well understood. One known example of directional dendritic transport are late endosomes moving retrogradel…

Collapse of late endosomal pH elicits a rapid Rab7 response via the V-ATPase and RILP Open

Ryan J. Mulligan, Magdalena M. Magaj, Laura Digilio, Stefanie Redemann, Chan Choo Yap , et al. · 2024

Endosomal-lysosomal trafficking is accompanied by the acidification of endosomal compartments by the H+-V-ATPase to reach low lysosomal pH. Disruption of the correct pH impairs lysosomal function and the balance of protein synthesis and de…

Collapse of late endosomal pH elicits a rapid Rab7 response via V-ATPase and RILP. Open

Ryan J. Mulligan, Magda M Magaj, Laura Digilio, Stefanie Redemann, Chan Choo Yap , et al. · 2023

Endosomal-lysosomal trafficking is accompanied by the acidification of endosomal compartments by the H+-V-ATPase to reach low lysosomal pH. Disruption of proper pH impairs lysosomal function and the balance of protein synthesis and degrada…

Disruption of Golgi markers by two RILP-directed shRNAs in neurons: A new role for RILP or a neuron-specific off-target phenotype? Open

Chan Choo Yap, Laura Digilio, Lloyd P. McMahon, Bettina Winckler · 2023

“Disruption of Golgi markers by two RILP-directed shRNAs in neurons: a new role for RILP or a neuron-specific off-target phenotype?” Open

Chan Choo Yap, Laura Digilio, Lloyd P. McMahon, Bettina Winckler · 2023

In neurons, degradation of dendritic cargos requires RAB7 and dynein-mediated retrograde transport to somatic lysosomes. In order to test if the dynein adaptor RILP (RAB-interacting lysosomal protein) mediated the recruitment of dynein to …

Quantifying Single and Dual Channel Live Imaging Data: Kymograph Analysis of Organelle Motility in Neurons Open

Laura Digilio, Lloyd P McMahon, Alois Duston, Chan Choo Yap, Bettina Winckler · 2023

Live imaging is commonly used to study dynamic processes in cells. Many labs carrying out live imaging in neurons use kymographs as a tool. Kymographs display time-dependent microscope data (time-lapsed images) in two-dimensional represent…

Expected and unexpected roles for dynein regulation of dendritic late endosomes Open

Chan Choo Yap, Bettina Winckler · 2022

Dendrites differ from axons in multiple ways, including the presence of minus-end out microtubules intermixed with the more conventional plus-end out microtubules. The mixed microtubule polarity makes regulation of directional transport in…

Validation of species-specificity of two commercial antibodies directed against RILP (Rab-interacting lyosomal protein) Open

Chan Choo Yap, Laura Digilio, Bettina Winckler · 2022

RILP is one of many effectors of RAB7 which bind to RAB7 in its activated GTP-bound state. The exact mechanism by which RAB7 effectors interact with RAB7 in time and space is not well understood. One of the known functions of RILP is to re…

Global loss of <span>Neuron‐specific</span> gene 1 causes alterations in motor coordination, increased anxiety, and diurnal hyperactivity in male mice Open

Roman Austin, Praveen Chander, Amber Zimmerman, Malene Overby, Laura Digilio , et al. · 2022

The Neuron‐specific gene family (NSG1‐3) consists of small endolysosomal proteins that are critical for trafficking multiple receptors and signaling molecules in neurons. NSG1 has been shown to play a critical role in AMPAR recycling from …

Dynein Is Required for Rab7-Dependent Endosome Maturation, Retrograde Dendritic Transport, and Degradation Open

Chan Choo Yap, Laura Digilio, Lloyd P. McMahon, Tuanlao Wang, Bettina Winckler · 2022

In all cell types, endocytosed cargo is transported along a set of endosomal compartments, which are linked maturationally from early endosomes (EEs) via late endosomes (LEs) to lysosomes. Lysosomes are critical for degradation of proteins…

Dynamics and distribution of endosomes and lysosomes in dendrites Open

Chan Choo Yap, Ashley J Mason, Bettina Winckler · 2022

Spatial regulation of endosomes in growing dendrites Open

Chan Choo Yap, Bettina Winckler · 2022

KymoMerge: a new tool for analysis of multichannel kymographs Open

Lloyd P. McMahon, Laura Digilio, Alois Duston, Chan Choo Yap, Bettina Winckler · 2021

Kymograph analysis is commonly used by researchers to study dynamic processes in cells. Current tools widely available only allow for analysis of one channel kymographs. Here we provide a python-coded, open source program to use as a plug-…

Lysosomes to the rescue: Anterograde axonal lysosome transport and neuronal proteostasis Open

Chan Choo Yap, Bettina Winckler · 2021

Nestin Selectively Facilitates the Phosphorylation of the Lissencephaly-Linked Protein Doublecortin (DCX) by cdk5/p35 to Regulate Growth Cone Morphology and Sema3a Sensitivity in Developing Neurons Open

Christopher J. Bott, Lloyd P. McMahon, Jason M. Keil, Chan Choo Yap, Kenneth Y. Kwan , et al. · 2020

Nestin, an intermediate filament protein widely used as a marker of neural progenitors, was recently found to be expressed transiently in developing cortical neurons in culture and in developing mouse cortex. In young cortical cultures, ne…

Nestin in immature embryonic neurons affects axon growth cone morphology and Semaphorin3a sensitivity Open

Christopher J. Bott, Colin G. Johnson, Chan Choo Yap, Noelle D. Dwyer, Karen Litwa , et al. · 2019

Correct wiring in the neocortex requires that responses to an individual guidance cue vary among neurons in the same location, and within the same neuron over time. Nestin is an atypical intermediate filament expressed strongly in neural p…

A dominant dendrite phenotype caused by the disease-associated G253D mutation in doublecortin (DCX) is not due to its endocytosis defect Open

Chan Choo Yap, Laura Digilio, Kamil Kruczek, Matylda Roszkowska, Xiaoqin Fu , et al. · 2018

Degradation of dendritic cargos requires Rab7-dependent transport to somatic lysosomes Open

Chan Choo Yap, Laura Digilio, Lloyd P. McMahon, A. Denise R. Garcia, Bettina Winckler · 2018

Neurons are large and long lived, creating high needs for regulating protein turnover. Disturbances in proteostasis lead to aggregates and cellular stress. We characterized the behavior of the short-lived dendritic membrane proteins Nsg1 a…

Transcytosis of TrkA leads to diversification of dendritic signaling endosomes Open

Kelly Barford, Austin B. Keeler, Lloyd P. McMahon, Kathryn F McDaniel, Chan Choo Yap , et al. · 2018

Nestin in immature embryonic neurons regulates axon growth cone morphology and Semaphorin3a sensitivity Open

Christopher J. Bott, Colin G. Johnson, Chan Choo Yap, Noelle D. Dwyer, Karen Litwa , et al. · 2017

Correct wiring in the neocortex requires that responses to an individual guidance cue vary among neurons in the same location, and within the same neuron over time. Nestin is an atypical intermediate filament expressed highly in neural pro…

Bulk degradation of dendritic cargos requires Rab7-dependent transport in Rab7-positive/LAMP1-negative endosomes to somatic lysosomes Open

Chan Choo Yap, Laura Digilio, Lawrence P. McMahon, A. Denise R. Garcia, Bettina Winckler · 2017

Regulation of protein homeostasis (“proteostasis”) is necessary for maintaining healthy cells. Disturbances in proteostasis lead to aggregates, cellular stress and can result in toxicity. There is thus great interest in when and where prot…

The endosomal neuronal proteins Nsg1/NEEP21 and Nsg2/P19 are itinerant, not resident proteins of dendritic endosomes Open

Chan Choo Yap, Laura Digilio, Lloyd P. McMahon, Bettina Winckler · 2017

Membrane traffic critically regulates most aspects of neuronal function. Neurons express many neuronal-specific proteins that regulate membrane traffic, including the poorly understood small transmembrane proteins neural-specific gene 1 an…

Different Doublecortin (DCX) Patient Alleles Show Distinct Phenotypes in Cultured Neurons Open

Chan Choo Yap, Laura Digilio, Lloyd P. McMahon, Matylda Roszkowska, Christopher J. Bott , et al. · 2016

Doublecortin on the X-chromosome (DCX) is a neuronal microtubule-binding protein with a multitude of roles in neurodevelopment. In humans, DCX is a major genetic locus for X-linked lissencephaly. The best studied defects are in neuronal mi…

Ctip2-, Satb2-, Prox1-, and GAD65-Expressing Neurons in Rat Cultures: Preponderance of Single- and Double-Positive Cells, and Cell Type-Specific Expression of Neuron-Specific Gene Family Members, Nsg-1 (NEEP21) and Nsg-2 (P19) Open

Laura Digilio, Chan Choo Yap, Bettina Winckler · 2015

The brain consists of many distinct neuronal cell types, but which cell types are present in widely used primary cultures of embryonic rodent brain is often not known. We characterized how abundantly four cell type markers (Ctip2, Satb2, P…

Adapting for endocytosis: roles for endocytic sorting adaptors in directing neural development Open

Chan Choo Yap, Bettina Winckler · 2015

Proper cortical development depends on the orchestrated actions of a multitude of guidance receptors and adhesion molecules and their downstream signaling. The levels of these receptors on the surface and their precise locations can greatl…

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