Explanipedia

Functional Nucleic Acid Probes Based on Two-Photon for Biosensing Open

Kefeng Wu, Changbei Ma, Yisen Wang · 2023

Functional nucleic acid (FNA) probes have been widely used in environmental monitoring, food analysis, clinical diagnosis, and biological imaging because of their easy synthesis, functional modification, flexible design, and stable propert…

A Fluorescent Biosensor for Streptavidin Detection Based on Double-Hairpin DNA-Templated Copper Nanoparticles Open

Qiangsheng Xiao, Mingjian Chen, Wanpin Nie, Fengjiao Xie, Xiao Yu , et al. · 2023

In this paper, we developed a sensitive, label-free and facile fluorescent strategy for detecting streptavidin (SA) based on double-hairpin DNA-templated copper nanoparticles (CuNPs) and terminal protection of small molecule-linked DNA. He…

Colorimetric Detection of Single-Nucleotide Mutation Using Rolling Circle Amplification and G-Quadruplex-Based DNAyme/Tetramethylbenzidine Open

Serge Yannick Ouédraogo, Moutanou Modeste Judes Zeye, Xi Zhou, Touwendpoulimdé Isabelle KIENDREBEOGO, Abdou Azaque Zouré , et al. · 2023

Recent Advances in Detection for Breast-Cancer-Derived Exosomes Open

Qin Tang, Xinying Xiao, Ranhao Li, Hailun He, Shanni Li , et al. · 2022

Breast cancer is the most common malignant tumor in women, its incidence is secret, and more than half of the patients are diagnosed in the middle and advanced stages, so it is necessary to develop simple and efficient detection methods fo…

A Label-Free Fluorescence Aptasensor Based on G-Quadruplex/Thioflavin T Complex for the Detection of Trypsin Open

Pan Gu, Yangfan Lu, Shanni Li, Changbei Ma · 2022

A novel, label-free fluorescent assay has been developed for the detection of trypsin by using thioflavin T as a fluorescent probe. A specific DNA aptamer can be combined by adding cytochrome c. Trypsin hydrolyzes the cytochrome c into sma…

Detection of DNA Methyltransferase Activity via Fluorescence Resonance Energy Transfer and Exonuclease-Mediated Target Recycling Open

Tingting Hu, Changbei Ma, Ying Yan, Junxiang Chen · 2022

In this study, a sensitive method for detecting DNA methyltransferase (MTase) activity was developed by combining the effective fluorescence resonance energy transfer (FRET) of cationic conjugated polymers and exonuclease (Exo) III–mediate…

Label-Free miRNA-21 Analysis Based on Strand Displacement and Terminal Deoxynucleotidyl Transferase-Assisted Amplification Strategy Open

Ying Yan, Han Zhao, Yukang Fang, Changbei Ma, Junxiang Chen · 2022

MicroRNAs (miRNAs) are regarded as a rising star in the biomedical industry. By monitoring slight increases in miRNA-21 levels, the possibilities of multi-type malignancy can be evaluated more precisely and earlier. However, the inconvenie…

A Label-Free Fluorometric Glutathione Assay Based on a Conformational Switch of G-quadruplex Open

Xi Zhou, Doudou Zhang, Ying Yan, Hailun He, Yukui Zhou , et al. · 2021

In this paper, a label-free fluorescent method for glutathione (GSH) detection based on a thioflavin T/G-quadruplex conformational switch is developed. The sensing assay is fabricated depending on the virtue of mercury ions to form a thymi…

An Exonuclease I-Aided Turn-Off Fluorescent Strategy for Alkaline Phosphatase Assay Based on Terminal Protection and Copper Nanoparticles Open

Yan Wang, Ying Yan, Xinfa Liu, Changbei Ma · 2021

As an important DNA 3′-phosphatase, alkaline phosphatase can repair damaged DNA caused by replication and recombination. It is essential to measure the level of alkaline phosphatase to indicate some potential diseases, such as cancer, rela…

Detection of Streptavidin Based on Terminal Protection and Cationic Conjugated Polymer-Mediated Fluorescence Resonance Energy Transfer Open

Tingting Hu, Ying Yan, Zhenwei Tang, Xinfa Liu, Changbei Ma · 2021

In this paper, a fast and simple strategy for sensitive detection of streptavidin (SA) was proposed based on terminal protection of small molecule-linked DNA and cationic conjugated polymer-mediated fluorescence resonance energy transfer (…

Sensitive Fluorescence Assay for the Detection of Alkaline Phosphatase Based on a Cu2+-Thiamine System Open

Han Zhao, Xinfa Liu, Changbei Ma · 2021

The authors describe a novel, facile, and sensitive fluorometric strategy based on a Cu2+-thiamine (Cu2+-TH) system for the detection of alkaline phosphatase (ALP) activity and inhibition. The principle of the method is as follows. Under a…

Amplified Fluorescent Aptasensor for Ochratoxin A Assay Based on Graphene Oxide and RecJf Exonuclease Open

Han Zhao, De-Hui Xiong, Ying Yan, Changbei Ma · 2020

In this study, we developed an aptamer-based fluorescent sensing platform for the detection of ochratoxin A (OTA) based on RecJf exonuclease-assisted signal amplification and interaction between graphene oxide (GO) and the OTA aptamer (OTA…

Mechanistic Insight into the Binding and Swelling Functions of Prepeptidase C-Terminal (PPC) Domains from Various Bacterial Proteases Open

Jiafeng Huang, RiBang Wu, Dan Liu, Binqiang Liao, Ming Lei , et al. · 2019

Prepeptidase C-terminal (PPC) domains commonly exist in the C termini of marine bacterial proteases. Reports examining PPC have been limited, and its functions remain unclear. In this study, eight PPCs from six different bacteria were exam…

Fluorescent Method for the Detection of Biothiols Using an Ag+-Mediated Conformational Switch Open

Han Zhao, Mingjian Chen, Changbei Ma · 2019

In this work, a novel, simple, and time-saving fluorescence approach for the detection of biothiols (glutathione and cysteine) was developed by employing a DNA probe labeled with 2-aminopurine. As an adenine analogue, 2-aminopurine exhibit…

Aptamer-Based Fluorometric Ochratoxin A Assay Based on Photoinduced Electron Transfer Open

Han Zhao, Xinying Xiang, Mingjian Chen, Changbei Ma · 2019

This study describes a novel quencher-free fluorescent method for ochratoxin A (OTA) detection based on the photoinduced electron transfer (PIET) between guanine and fluorophore. In the absence of OTA, carboxyfluorescein (FAM)-labeled apta…

A Label-Free Fluorescent Assay for the Rapid and Sensitive Detection of Adenosine Deaminase Activity and Inhibition Open

Xinxing Tang, Kefeng Wu, Han Zhao, Mingjian Chen, Changbei Ma · 2018

Adenosine deaminase (ADA), able to catalyze the irreversible deamination of adenosine into inosine, can be found in almost all tissues and plays an important role in several diseases. In this work, we developed a label-free fluorescence me…

Label-Free G-Quadruplex Aptamer Fluorescence Assay for Ochratoxin A Using a Thioflavin T Probe Open

Kefeng Wu, Changbei Ma, Han Zhao, Hailun He, Hanchun Chen · 2018

Ochratoxin A (OTA) is one of the most common mycotoxins contaminating feed and foodstuffs. Therefore, a great deal of concern is associated with AFB1 toxicity. In this work, a fast and sensitive fluorescence aptamer biosensor has been prop…

Two-Color-Based Nanoflares for Multiplexed MicroRNAs Imaging in Live Cells Open

Jing Li, Jin Huang, Xiaohai Yang, Yanjing Yang, Ke Quan , et al. · 2017

MicroRNAs (miRNAs) have become an ideal biomarker candidate for early diagnosis of diseases. But various diseases involve changes in the expression of different miRNAs. Therefore, multiplexed assay of miRNAs in live cells can provide criti…

A Novel Detection Method of Human Serum Albumin Based on the Poly(Thymine)-Templated Copper Nanoparticles Open

Mingjian Chen, Xinying Xiang, Kefeng Wu, Hailun He, Hanchun Chen , et al. · 2017

In this work, we developed a facile fluorescence method for quantitative detection of human serum albumin (HSA) based on the inhibition of poly(thymine) (poly T)-templated copper nanoparticles (CuNPs) in the presence of HSA. Under normal c…

Simple fluorescence-based detection of protein kinase A activity using a molecular beacon probe Open

Changbei Ma, Xiaoyuan Lv, Kemin Wang, Shunxin Jin, Haisheng Liu , et al. · 2017

Protein kinase A was detected by quantifying the amount of ATP used after a protein kinase reaction. The ATP assay was performed using the T4 DNA ligase and a molecular beacon (MB). In the presence of ATP, DNA ligase catalyzed the ligation…

An Exonuclease I-Based Quencher-Free Fluorescent Method Using DNA Hairpin Probes for Rapid Detection of MicroRNA Open

Changbei Ma, Haisheng Liu, Kefeng Wu, Mingjian Chen, Liyang Zheng , et al. · 2017

MicroRNAs (miRNAs) act as biomarkers for the diagnosis of a variety of cancers. Since the currently used methods for miRNA detection have limitations, simple, sensitive, and cost-effective methods for the detection of miRNA are required. T…

Quencher-Free Fluorescence Method for the Detection of Mercury(II) Based on Polymerase-Aided Photoinduced Electron Transfer Strategy Open

Haisheng Liu, Linbin Ma, Changbei Ma, Junyan Du, Meilan Wang , et al. · 2016

A new quencher-free Hg2+ ion assay method was developed based on polymerase-assisted photoinduced electron transfer (PIET). In this approach, a probe is designed with a mercury ion recognition sequence (MRS) that is composed of two T-rich …

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