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View article: Unraveling the future of genomics: CRISPR, single-cell omics, and the applications in cancer and immunology
Unraveling the future of genomics: CRISPR, single-cell omics, and the applications in cancer and immunology Open
The CRISPR system has transformed many research areas, including cancer and immunology, by providing a simple yet effective genome editing system. Its simplicity has facilitated large-scale experiments to assess gene functionality across d…
View article: Decoding heterogeneous single-cell perturbation responses
Decoding heterogeneous single-cell perturbation responses Open
View article: A comprehensive benchmark for multiple highly efficient base editors with broad targeting scope
A comprehensive benchmark for multiple highly efficient base editors with broad targeting scope Open
As the toolbox of base editors (BEs) expands, selecting appropriate BE and guide RNA (gRNA) to achieve optimal editing efficiency and outcome for a given target becomes challenging. Here, we construct a set of 10 adenine and cytosine BEs w…
View article: Assessing water use efficiency reactivity to meteorological, hydrological, and agricultural droughts on the Mongolian Plateau
Assessing water use efficiency reactivity to meteorological, hydrological, and agricultural droughts on the Mongolian Plateau Open
Water use efficiency (WUE) is an important metric for assessing regional sustainability and the carbon-water balance of ecosystems. Drought is a significant stressor affecting the carbon-water cycle. Nevertheless, the response of WUE to di…
View article: Decoding Heterogenous Single-cell Perturbation Responses
Decoding Heterogenous Single-cell Perturbation Responses Open
Understanding diverse responses of individual cells to the same perturbation is central to many biological and biomedical problems. Current methods, however, do not precisely quantify the strength of perturbation responses and, more import…
View article: Effects of Vegetation Belt Movement on Wildfire in the Mongolian Plateau over the Past 40 Years
Effects of Vegetation Belt Movement on Wildfire in the Mongolian Plateau over the Past 40 Years Open
The frequency and intensity of fires are increasing because of warmer temperatures and increased droughts, as well as climate-change induced fuel distribution changes. Vegetation in environments, such as those in the mid-to-high latitudes …
View article: Modeling CRISPR-Cas13d on-target and off-target effects using machine learning approaches
Modeling CRISPR-Cas13d on-target and off-target effects using machine learning approaches Open
View article: Ag85A, As an S2 Vaccine Carrier, Reduces the Toxicity of the S2 Vaccine and Enhances the Protective Ability of Mice against Brucella
Ag85A, As an S2 Vaccine Carrier, Reduces the Toxicity of the S2 Vaccine and Enhances the Protective Ability of Mice against Brucella Open
Brucella is a globally distributed zoonotic disease that can cause abortion and changes in immune function in humans and animals. At present, there is no good treatment plan for Brucella, and animals can only be treated harmlessly once the…
View article: Temperature modulation of <scp>CAMTA3</scp> gene induction activity is mediated through the <scp>DNA</scp> binding domain
Temperature modulation of <span>CAMTA3</span> gene induction activity is mediated through the <span>DNA</span> binding domain Open
SUMMARY The calmodulin‐binding transcription activator (CAMTA) proteins of Arabidopsis thaliana play a major role in cold acclimation, contributing to the rapid induction of the C‐REPEAT BINDING FACTOR ( CBF ) genes and other genes that im…
View article: Intrinsic RNA targeting constrains the utility of CRISPR-Cas13 systems
Intrinsic RNA targeting constrains the utility of CRISPR-Cas13 systems Open
SUMMARY CRISPR-Cas13 systems have been adapted as versatile toolkits for RNA-related applications. Here we systematically evaluate the performance of several popular Cas13 family effectors (Cas13a, Cas13b and Cas13d) under lentiviral vecto…
View article: Modeling CRISPR-Cas13d on-target and off-target effects using machine learning approaches
Modeling CRISPR-Cas13d on-target and off-target effects using machine learning approaches Open
A major challenge in the application of the CRISPR-Cas13d (RfxCas13d, or CasRx) RNA editing system is to accurately predict its guide RNA (gRNA) dependent on-target and off-target effect. Here, we performed CRISPR-Cas13d proliferation scre…
View article: CRISP-view: a database of functional genetic screens spanning multiple phenotypes
CRISP-view: a database of functional genetic screens spanning multiple phenotypes Open
High-throughput genetic screening based on CRISPR/Cas9 or RNA-interference (RNAi) enables the exploration of genes associated with the phenotype of interest on a large scale. The rapid accumulation of public available genetic screening dat…
View article: Arabidopsis CAMTA Transcription Factors Regulate Pipecolic Acid Biosynthesis and Priming of Immunity Genes
Arabidopsis CAMTA Transcription Factors Regulate Pipecolic Acid Biosynthesis and Priming of Immunity Genes Open
View article: Arabidopsis Transcription Factors SPL1 and SPL12 Confer Plant Thermotolerance at Reproductive Stage
Arabidopsis Transcription Factors SPL1 and SPL12 Confer Plant Thermotolerance at Reproductive Stage Open