Charelle Boot
YOU?
Author Swipe
View article: Description and functional validation of human enteroendocrine cell sensors
Description and functional validation of human enteroendocrine cell sensors Open
Enteroendocrine cells (EECs) are gut epithelial cells that respond to intestinal contents by secreting hormones, including the incretins glucagon-like peptide 1 (GLP-1) and gastric inhibitory protein (GIP), which regulate multiple physiolo…
View article: Mapping prohormone processing by proteases in human enteroendocrine cells using genetically engineered organoid models
Mapping prohormone processing by proteases in human enteroendocrine cells using genetically engineered organoid models Open
Enteroendocrine cells (EECs) secrete hormones in response to ingested nutrients to control physiological processes such as appetite and insulin release. EEC hormones are synthesized as large proproteins that undergo proteolytic processing …
View article: Differentiation and CRISPR-Cas9-mediated genetic engineering of human intestinal organoids
Differentiation and CRISPR-Cas9-mediated genetic engineering of human intestinal organoids Open
Intestinal organoids are three-dimensional cultures that resemble key aspects of the epithelium of origin. Here, we describe how to differentiate human small intestinal organoids by combining growth media variations and genetic engineering…
View article: Colon Tumors in Enterotoxigenic Bacteroides fragilis (ETBF)-Colonized Mice Do Not Display a Unique Mutational Signature but Instead Possess Host-Dependent Alterations in the APC Gene
Colon Tumors in Enterotoxigenic Bacteroides fragilis (ETBF)-Colonized Mice Do Not Display a Unique Mutational Signature but Instead Possess Host-Dependent Alterations in the APC Gene Open
Many studies have shown that microbiome composition in both the mucosa and the stool differs in individuals with sporadic and hereditary colorectal cancer (CRC). Both human and mouse models have established a strong association between par…
View article: BMP gradient along the intestinal villus axis controls zonated enterocyte and goblet cell states
BMP gradient along the intestinal villus axis controls zonated enterocyte and goblet cell states Open
Intestinal epithelial cells derive from stem cells at the crypt base and travel along the crypt-villus axis to die at the villus tip. The two dominant villus epithelial cell types, absorptive enterocytes and mucous-secreting goblet cells, …
View article: The in vitro direct mycobacterial growth inhibition assay (MGIA) for the early evaluation of TB vaccine candidates and assessment of protective immunity: a protocol for non-human primate cells
The in vitro direct mycobacterial growth inhibition assay (MGIA) for the early evaluation of TB vaccine candidates and assessment of protective immunity: a protocol for non-human primate cells Open
The only currently available approach to early efficacy testing of tuberculosis (TB) vaccine candidates isin vivopreclinical challenge models. These typically include mice, guinea pigs and non-human primates (NHPs), which must be exposed t…
View article: The in vitro direct mycobacterial growth inhibition assay (MGIA) for the early evaluation of TB vaccine candidates and assessment of protective immunity: a protocol for non-human primate cells
The in vitro direct mycobacterial growth inhibition assay (MGIA) for the early evaluation of TB vaccine candidates and assessment of protective immunity: a protocol for non-human primate cells Open
The only currently available approach to early efficacy testing of tuberculosis (TB) vaccine candidates isin vivopreclinical challenge models. These typically include mice, guinea pigs and non-human primates (NHPs), which must be exposed t…
View article: Pulmonary MTBVAC vaccination induces immune signatures previously correlated with prevention of tuberculosis infection
Pulmonary MTBVAC vaccination induces immune signatures previously correlated with prevention of tuberculosis infection Open
To fight tuberculosis, better vaccination strategies are needed. Live attenuated Mycobacterium tuberculosis-derived vaccine, MTBVAC, is a promising candidate in the pipeline, proven to be safe and immunogenic in humans so far. Independent …
View article: Stronger induction of trained immunity by mucosal BCG or MTBVAC vaccination compared to standard intradermal vaccination
Stronger induction of trained immunity by mucosal BCG or MTBVAC vaccination compared to standard intradermal vaccination Open
BCG vaccination can strengthen protection against pathogens through the induction of epigenetic and metabolic reprogramming of innate immune cells, a process called trained immunity. We and others recently demonstrated that mucosal or intr…
View article: Disparate Tuberculosis Disease Development in Macaque Species Is Associated With Innate Immunity
Disparate Tuberculosis Disease Development in Macaque Species Is Associated With Innate Immunity Open
While tuberculosis continues to afflict mankind, the immunological mechanisms underlying TB disease development are still incompletely understood. Advanced preclinical models for TB research include both rhesus and cynomolgus macaques (Mac…