Charlotte Rimbault
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View article: Paralog-specific intrabodies for PSD-93 and SAP102 expand the molecular toolkit to resolve excitatory synapse organization
Paralog-specific intrabodies for PSD-93 and SAP102 expand the molecular toolkit to resolve excitatory synapse organization Open
A scarcity of live, paralog-specific tools has limited analysis of PSD-MAGUKs at excitatory synapses. To address this gap, we engineered small, 10 FN3-derived binders that selectively recognize PSD-93 and SAP102 -alongside an enhanced PSD-…
View article: A comparative study of the performance of E. coli and K. phaffii for expressing α-cobratoxin
A comparative study of the performance of E. coli and K. phaffii for expressing α-cobratoxin Open
View article: Engineering paralog-specific PSD-95 recombinant binders as minimally interfering multimodal probes for advanced imaging techniques
Engineering paralog-specific PSD-95 recombinant binders as minimally interfering multimodal probes for advanced imaging techniques Open
Despite the constant advances in fluorescence imaging techniques, monitoring endogenous proteins still constitutes a major challenge in particular when considering dynamics studies or super-resolution imaging. We have recently evolved spec…
View article: Phage display assisted discovery of a <scp>pH</scp> ‐dependent anti‐α‐cobratoxin antibody from a natural variable domain library
Phage display assisted discovery of a <span>pH</span> ‐dependent anti‐α‐cobratoxin antibody from a natural variable domain library Open
Recycling IgG antibodies bind to their target antigen at physiological pH in the blood stream and release them upon endocytosis when pH levels drop, allowing the IgG antibodies to be recycled into circulation via FcRn‐mediated cellular pat…
View article: Author response: Engineering paralog-specific PSD-95 recombinant binders as minimally interfering multimodal probes for advanced imaging techniques
Author response: Engineering paralog-specific PSD-95 recombinant binders as minimally interfering multimodal probes for advanced imaging techniques Open
View article: Phage display assisted discovery of a pH-dependent anti-α-cobratoxin antibody from a natural variable domain library
Phage display assisted discovery of a pH-dependent anti-α-cobratoxin antibody from a natural variable domain library Open
Recycling antibodies can bind to their target antigen at neutral pH in the blood stream and release them upon endocytosis when pH levels drop, allowing the antibodies to be recycled into circulation via FcRn-mediated pathway, while the ant…
View article: A single-chain variable fragment selected against a conformational epitope of a recombinantly produced snake toxin using phage display
A single-chain variable fragment selected against a conformational epitope of a recombinantly produced snake toxin using phage display Open
Phage display technology is a powerful tool for selecting monoclonal antibodies against a diverse set of antigens. Within toxinology, however, it remains challenging to generate monoclonal antibodies against many animal toxins, as they are…
View article: Generation of Multivalent Nanobody-Based Proteins with Improved Neutralization of Long α-Neurotoxins from Elapid Snakes
Generation of Multivalent Nanobody-Based Proteins with Improved Neutralization of Long α-Neurotoxins from Elapid Snakes Open
Recombinantly produced biotherapeutics hold promise for improving the current standard of care for snakebite envenoming over conventional serotherapy. Nanobodies have performed well in the clinic, and in the context of antivenom, they have…
View article: Advances in antibody phage display technology
Advances in antibody phage display technology Open
Phage display technology can be used for the discovery of antibodies for research, diagnostic, and therapeutic purposes. In this review, we present and discuss key parameters that can be optimized when performing phage display selection ca…
View article: Strategies for Heterologous Expression, Synthesis, and Purification of Animal Venom Toxins
Strategies for Heterologous Expression, Synthesis, and Purification of Animal Venom Toxins Open
Animal venoms are complex mixtures containing peptides and proteins known as toxins, which are responsible for the deleterious effect of envenomations. Across the animal Kingdom, toxin diversity is enormous, and the ability to understand t…
View article: Engineering paralog-specific PSD-95 synthetic binders as potent and minimally invasive imaging probes
Engineering paralog-specific PSD-95 synthetic binders as potent and minimally invasive imaging probes Open
Despite the constant advances in fluorescence imaging techniques, monitoring endogenous proteins still constitutes a major challenge in particular when considering dynamics studies or super-resolution imaging. We have recently evolved spec…
View article: Current Knowledge on Snake Dry Bites
Current Knowledge on Snake Dry Bites Open
Snake ‘dry bites’ are characterized by the absence of venom being injected into the victim during a snakebite incident. The dry bite mechanism and diagnosis are quite complex, and the lack of envenoming symptoms in these cases may be misin…
View article: Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modules
Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modules Open
Designing highly specific modulators of protein-protein interactions (PPIs) is especially challenging in the context of multiple paralogs and conserved interaction surfaces. In this case, direct generation of selective and competitive inhi…
View article: Modulation des interactions impliquant les domaines PDZ par une approche d’évolution dirigée
Modulation des interactions impliquant les domaines PDZ par une approche d’évolution dirigée Open
Les interactions protéine-protéine (IPPs), complexes et dynamiques, sont le cœur des réseaux protéiques cellulaires. Au niveau des synapses excitatrices, la densité post-synaptique (PSD) est un exemple typique de réseau protéique dont la s…