Explanipedia

A telomere-to-telomere gap-free genome assembly of the endangered humphead wrasse (Cheilinus undulatus) Open

Kai Zhang, Jianchao Chen, Binwei Duan, Chenjiang You, Wenchuan Zhou , et al. · 2025

Genome-Wide Identification of DNA Methyltransferases (Dnmts) in Fish and Its Potential Roles During Sex Change in Blackhead Seabream Open

Shilong Guo, Binwei Duan, Jianchao Chen, Mingyang Cui, Chenjiang You , et al. · 2025

DNA methylation, also known as 5-methylcytosine, is an epigenetic modification that has crucial functions in multiple important biological processes in fish, such as gonadal development. The cellular DNA methylation level is tightly regula…

Ribosomes binding to TAS transcripts buffer ta-siRNA biogenesis in Arabidopsis thaliana Open

Han Wang, Qiming Wen, Minglei Zhao, Hualong He, Jie Cui , et al. · 2025

Small RNAs, including ta-siRNAs, play crucial roles in various processes in plants. Efforts have been made for decades to elucidate the biogenesis and function of ta-siRNAs. Though the key proteins involved in ta-siRNA biogenesis have been…

EccDNA Analysis Provides Novel Insights Into the Molecular Mechanism of Firmness of Fish Fillet Open

Kai Zhang, Jianchao Chen, Haobin He, Binwei Duan, Chenjiang You , et al. · 2025

Extrachromosomal circular DNAs (eccDNAs) play a significant role in regulating various biological processes, including abnormal muscle development. The molecular functions and impact of eccDNAs in the muscle development of fish are poorly …

HOT3/eIF5B1 confers Kozak motif-dependent translational control of photosynthesis-associated nuclear genes for chloroplast biogenesis Open

Runlai Hang, Hao Li, Wenjing Liu, Runyu Wang, Hao Hu , et al. · 2024

Photosynthesis requires chloroplasts, in which most proteins are nucleus-encoded and produced via cytoplasmic translation. The translation initiation factor eIF5B gates the transition from initiation (I) to elongation (E), and the Kozak mo…

HEI10 is subject to phase separation and mediates RPA1a degradation during meiotic interference-sensitive crossover formation Open

Tianyi Wang, Hongkuan Wang, Qichao Lian, Qian Jia, Chenjiang You , et al. · 2023

Reciprocal exchanges of DNA between homologous chromosomes during meiosis, or crossovers (COs), shuffle genetic information in gametes and progeny. In many eukaryotes, the majority of COs (class I COs) are sensitive to a phenomenon called …

SCFRMF mediates degradation of the meiosis-specific recombinase DMC1 Open

Wanyue Xu, Yue Yu, Juli Jing, Zhen Wu, Xumin Zhang , et al. · 2023

Arabidopsis HOT3/eIF5B1 constrains rRNA RNAi by facilitating 18S rRNA maturation Open

Runlai Hang, Ye Xu, Xufeng Wang, Hao Hu, Nora Flynn , et al. · 2023

Ribosome biogenesis is essential for protein synthesis in gene expression. Yeast eIF5B has been shown biochemically to facilitate 18S ribosomal RNA (rRNA) 3′ end maturation during late-stage 40S ribosomal subunit assembly and gate the tran…

Anterograde signaling controls plastid transcription via sigma factors separately from nuclear photosynthesis genes Open

Youra Hwang, Soeun Han, Chan Yul Yoo, Hong Liu, Chenjiang You , et al. · 2022

Light initiates chloroplast biogenesis in Arabidopsis by eliminating PHYTOCHROME-INTERACTING transcription FACTORs (PIFs), which in turn de-represses nuclear photosynthesis genes, and synchronously, generates a nucleus-to-plastid (anterogr…

ZMP recruits and excludes Pol IV–mediated DNA methylation in a site-specific manner Open

Yuan Wang, Brandon H. Le, Jian‐Qiang Wang, Chenjiang You, Yonghui Zhao , et al. · 2022

In plants, RNA-directed DNA methylation (RdDM) uses small interfering RNAs (siRNAs) to target transposable elements (TEs) but usually avoids genes. RNA polymerase IV (Pol IV) shapes the landscape of DNA methylation through its pivotal role…

DNA polymerase epsilon interacts with SUVH2/9 to repress the expression of genes associated with meiotic DSB hotspot in Arabidopsis Open

Cong Wang, Jiyue Huang, Jun Zhang, Yue Yu, Gregory P. Copenhaver , et al. · 2022

Meiotic recombination is initiated by the SPORULATION 11 (SPO11)–triggered formation of double-strand breaks (DSBs) that usually occur in open chromatin with active transcriptional features in many eukaryotes. However, gene transcription a…

Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis Open

Lusheng Fan, Bin Gao, Ye Xu, Nora Flynn, Brandon H. Le , et al. · 2022

MicroRNAs (miRNAs) play an essential role in plant growth and development, and as such, their biogenesis is fine-tuned via regulation of the core microprocessor components. Here, we report that Arabidopsis AAR2, a homolog of a U5 snRNP ass…

A spontaneous thermo-sensitive female sterility mutation in rice enables fully mechanized hybrid breeding Open

Haoxuan Li, Chenjiang You, Manabu Yoshikawa, Xiaoyu Yang, Haiyong Gu , et al. · 2022

Parallel analysis of RNA ends reveals global microRNA ‐mediated target RNA cleavage in maize Open

Juan He, Chi Xu, Chenjiang You, Beixin Mo, Xuemei Chen , et al. · 2022

SUMMARY MicroRNAs (miRNAs) are endogenous 20–24‐nucleotide non‐coding RNAs that play important regulatory roles in many biological processes in eukaryotes. miRNAs modulate the expression of target genes at the post‐transcriptional level by…

Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis Open

Lusheng Fan, Bin Gao, Ye Xu, Nora Flynn, Brandon H. Le , et al. · 2022

MicroRNAs (miRNAs) play an essential role in plant growth and development, and as such, their biogenesis is fine-tuned via regulation of the core microprocessor components. Here, we report that Arabidopsis AAR2, a homolog of a U5 snRNP ass…

Parallel analysis of RNA ends reveals global microRNA-mediated target RNA cleavage in maize Open

Juan He, Chi Xu, Chenjiang You, Beixin Mo, Xuemei Chen , et al. · 2022

MicroRNAs (miRNAs) are endogenous 20-to 24-nucleotide (nt) noncoding RNAs that play important regulatory roles in many biological processes in eukaryotes. miRNAs modulate the expression of target genes at the post-transcriptional level by …

Small RNA in plant meiosis and gametogenesis Open

Chenjiang You, Yue Yu, Yingxiang Wang · 2022

Small RNAs are short non-coding nucleotides and regulate various biological processes in eukaryotes. During past decades, numerous studies have demonstrated the biogenesis of small RNAs and their modes of action in regulating gene expressi…

T-LOC: A comprehensive tool to localize and characterize T-DNA integration sites Open

Shaofang Li, Chenyang Wang, Chenjiang You, Xueping Zhou, Huanbin Zhou · 2022

Scientists have developed many approaches based on PCR or next-generation sequencing to localize and characterize integrated T-DNAs in transgenic plants generated by Agrobacterium tumefaciens-mediated T-DNA transfer. However, none of these…

Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading Open

C. Liang, Qiang Cai, Fei Wang, Shaofang Li, Chenjiang You , et al. · 2022

RBV, an evolutionarily conserved WD40 repeat protein, promotes microRNA biogenesis and loading into ARGONAUTE1 in Arabidopsis Open

Chao Liang, Qiang Cai, Shaofang Li, Chenjiang You, Chi Xu , et al. · 2021

MicroRNAs (miRNAs) play crucial roles in gene expression regulation through RNA cleavage or translation repression. Although miRNA biogenesis has been extensively studied, new factors involved in miRNA biogenesis are still being found from…

SPAAC-NAD-seq, a sensitive and accurate method to profile NAD + -capped transcripts Open

Hao Hu, Nora Flynn, Hailei Zhang, Chenjiang You, Runlai Hang , et al. · 2021

Significance The m 7 G cap is the canonical RNA cap in eukaryotes, but other noncanonical RNA caps exist, including the NAD + cap. NAD captureSeq has been widely used to profile NAD + -capped RNAs (NAD-RNAs) in prokaryotes and eukaryotes. …

Widespread occurrence of microRNA-mediated target cleavage on membrane-bound polysomes Open