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View article: Aberration correction in long GRIN lens-based microendoscopes for extended field-of-view two-photon imaging in deep brain regions
Aberration correction in long GRIN lens-based microendoscopes for extended field-of-view two-photon imaging in deep brain regions Open
Two-photon (2P) fluorescence imaging through gradient index (GRIN) lens-based endoscopes is fundamental to investigate the functional properties of neural populations in deep brain circuits. However, GRIN lenses have intrinsic optical aber…
View article: Aberration correction in long GRIN lens-based microendoscopes for extended field-of-view two-photon imaging in deep brain regions
Aberration correction in long GRIN lens-based microendoscopes for extended field-of-view two-photon imaging in deep brain regions Open
Two-photon (2P) fluorescence imaging through gradient index (GRIN) lens-based endoscopes is fundamental to investigate the functional properties of neural populations in deep brain circuits. However, GRIN lenses have intrinsic optical aber…
View article: Aberration correction in long GRIN lens-based microendoscopes for extended field-of-view two-photon imaging in deep brain regions
Aberration correction in long GRIN lens-based microendoscopes for extended field-of-view two-photon imaging in deep brain regions Open
Two-photon (2P) fluorescence imaging through gradient index (GRIN) lens-based endoscopes is fundamental to investigate the functional properties of neural populations in deep brain circuits. However, GRIN lenses have intrinsic optical aber…
View article: Aberration correction in long GRIN lens-based microendoscopes for extended field-of-view two-photon imaging in deep brain regions
Aberration correction in long GRIN lens-based microendoscopes for extended field-of-view two-photon imaging in deep brain regions Open
Two-photon (2P) fluorescence imaging through gradient index (GRIN) lens-based endoscopes is fundamental to investigate the functional properties of neural populations in deep brain circuits. However, GRIN lenses have intrinsic optical aber…
View article: Aberration correction in long GRIN lens-based microendoscopes for extended field-of-view two-photon imaging in deep brain regions
Aberration correction in long GRIN lens-based microendoscopes for extended field-of-view two-photon imaging in deep brain regions Open
Two-photon (2P) fluorescence imaging through gradient index (GRIN) lens-based endoscopes is fundamental to investigate the functional properties of neural populations in deep brain circuits. However, GRIN lenses have intrinsic optical aber…
View article: Aberration correction in long GRIN lens-based microendoscopes for extended field-of-view two-photon imaging in deep brain regions
Aberration correction in long GRIN lens-based microendoscopes for extended field-of-view two-photon imaging in deep brain regions Open
Two-photon (2P) fluorescence imaging through gradient index (GRIN) lens-based endoscopes is fundamental to investigate the functional properties of neural populations in deep brain circuits. However, GRIN lenses have intrinsic optical aber…
View article: A Quantitative Assay for Ca2+ Uptake through Normal and Pathological Hemichannels
A Quantitative Assay for Ca2+ Uptake through Normal and Pathological Hemichannels Open
Connexin (Cx) hemichannels (HCs) are large pore hexameric structures that allow the exchange of ions, metabolites and a variety of other molecules between the cell cytoplasm and extracellular milieu. HC inhibitors are attracting growing in…
View article: Commercially derived versatile optical architecture for two-photon STED, wavelength mixing and label-free microscopy
Commercially derived versatile optical architecture for two-photon STED, wavelength mixing and label-free microscopy Open
Multimodal microscopy combines multiple non-linear techniques that take advantage of different optical processes to generate contrast and increase the amount of information that can be obtained from biological samples. However, the most ad…
View article: Calcium Signaling in the Photodamaged Skin: In Vivo Experiments and Mathematical Modeling
Calcium Signaling in the Photodamaged Skin: In Vivo Experiments and Mathematical Modeling Open
The epidermis forms an essential barrier against a variety of insults. The overall goal of this study was to shed light not only on the effects of accidental epidermal injury, but also on the mechanisms that support laser skin resurfacing …
View article: Connexin Hemichannel Activation by S-Nitrosoglutathione Synergizes Strongly with Photodynamic Therapy Potentiating Anti-Tumor Bystander Killing
Connexin Hemichannel Activation by S-Nitrosoglutathione Synergizes Strongly with Photodynamic Therapy Potentiating Anti-Tumor Bystander Killing Open
In this study, we used B16-F10 cells grown in the dorsal skinfold chamber (DSC) preparation that allowed us to gain optical access to the processes triggered by photodynamic therapy (PDT). Partial irradiation of a photosensitized melanoma …
View article: How to Extend the Capabilities of a Commercial Two-Photon Microscope to Perform Super-Resolution Imaging, Wavelength Mixing and Label-Free Microscopy
How to Extend the Capabilities of a Commercial Two-Photon Microscope to Perform Super-Resolution Imaging, Wavelength Mixing and Label-Free Microscopy Open
Multimodal microscopy combines multiple non-linear techniques that take advantage of different optical processes to generate contrast and increase the amount of information that can be obtained from biological samples. However, the most ad…
View article: Calcium signaling in the photodamaged skin
Calcium signaling in the photodamaged skin Open
BACKGROUND The mammalian skin, the body’s largest single organ, is a highly organized tissue that forms an essential barrier against dehydration, pathogens, light and mechanical injury. Damage triggers perturbations of the cytosolic free C…
View article: Role of vascular adaptation in determining systolic BP in young adults
Role of vascular adaptation in determining systolic BP in young adults Open
Article published in Journal of the American Heart Association, available open access at: https://doi.org/10.1161/JAHA.119.014375.
View article: Organ-on-chip model shows that ATP release through connexin hemichannels drives spontaneous Ca<sup>2+</sup> signaling in non-sensory cells of the greater epithelial ridge in the developing cochlea
Organ-on-chip model shows that ATP release through connexin hemichannels drives spontaneous Ca<sup>2+</sup> signaling in non-sensory cells of the greater epithelial ridge in the developing cochlea Open
Using microfluidics, ATP biosensors, multiphoton microscopy and genetically targeted mice, we show that ATP release through connexin hemichannels, and not pannexin 1 channels, underlies spontaneous Ca2+ wave propagation in the greater epit…
View article: Photosensitizer Activation Drives Apoptosis by Interorganellar Ca2+ Transfer and Superoxide Production in Bystander Cancer Cells
Photosensitizer Activation Drives Apoptosis by Interorganellar Ca2+ Transfer and Superoxide Production in Bystander Cancer Cells Open
In cells, photosensitizer (PS) activation by visible light irradiation triggers reactive oxygen species (ROS) formation, followed by a cascade of cellular responses involving calcium (Ca2+) and other second messengers, resulting in cell de…
View article: A Human-Derived Monoclonal Antibody Targeting Extracellular Connexin Domain Selectively Modulates Hemichannel Function
A Human-Derived Monoclonal Antibody Targeting Extracellular Connexin Domain Selectively Modulates Hemichannel Function Open
Connexin hemichannels, which are plasma membrane hexameric channels (connexons) composed of connexin protein protomers, have been implicated in a host of physiological processes and pathological conditions. A number of single point patholo…
View article: Warfarin, but not rivaroxaban, promotes the calcification of the aortic valve in ApoE−/− mice
Warfarin, but not rivaroxaban, promotes the calcification of the aortic valve in ApoE−/− mice Open
Summary Introduction Vitamin K antagonists, such as warfarin, are known to promote arterial calcification through blockade of gamma‐carboxylation of Matrix‐Gla‐Protein. It is currently unknown whether other oral anticoagulants such as dire…
View article: Mouse Panx1 Is Dispensable for Hearing Acquisition and Auditory Function
Mouse Panx1 Is Dispensable for Hearing Acquisition and Auditory Function Open
Panx1 forms plasma membrane channels in brain and several other organs, including the inner ear. Biophysical properties, activation mechanisms and modulators of Panx1 channels have been characterized in detail, however the impact of Panx1 …
View article: Design and Characterization of a Human Monoclonal Antibody that Modulates Mutant Connexin 26 Hemichannels Implicated in Deafness and Skin Disorders
Design and Characterization of a Human Monoclonal Antibody that Modulates Mutant Connexin 26 Hemichannels Implicated in Deafness and Skin Disorders Open
Background: Mutations leading to changes in properties, regulation, or expression of connexin-made channels have been implicated in 28 distinct human hereditary diseases. Eight of these result from variants of connexin 26 (Cx26), a protein…