Chie Mori
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View article: Effect of preoperative oral rehydration before cesarean section on ultrasound assessment of gastric volume and intraoperative hemodynamic changes: a randomized controlled trial
Effect of preoperative oral rehydration before cesarean section on ultrasound assessment of gastric volume and intraoperative hemodynamic changes: a randomized controlled trial Open
Background Cesarean section often requires an urgent transfusion load due to decreased blood pressure after spinal anesthesia. This prospective randomized study aimed to investigate whether a preoperative oral rehydration solution (ORS) st…
View article: Acute exacerbation of pulmonary sarcoidosis preceded by neuropathic pain： a case report
Acute exacerbation of pulmonary sarcoidosis preceded by neuropathic pain： a case report Open
症例は 63歳，女性．右胸背部痛を主訴に近医を受診した。胸部 CTで両側肺門リンパ節腫脹を認め，サルコイドーシスが疑われ，精査加療目的に当科へ入院した．体幹痛は著明でありフェンタニル貼付薬を開始した．両肘両膝に扁平隆起性の皮疹を認め，皮膚生検を施行した．皮膚生検の病理診断は非乾酪性類上皮細胞肉芽腫であり，サルコイドーシスと確定診断した．入院 12日目に酸素化の増悪を認め，胸部 CTで両肺間質陰影の増悪を認めたため，酸素投与を開始した．プレドニゾロン（ PSL） 60mg/日…
View article: Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase
Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase Open
DNA transfection is an important technology in the life sciences, wherein nuclear entry of DNA is necessary to achieve expression of the exogenous DNA. Non-viral vectors and their transfection reagents are useful as safe tools for transfec…
View article: Microtubule inhibitors identified through non-biased screening enhance DNA transfection efficiency by delaying p62-dependent ubiquitin recruitment
Microtubule inhibitors identified through non-biased screening enhance DNA transfection efficiency by delaying p62-dependent ubiquitin recruitment Open
Ectopic gene expression is an indispensable tool in biology and medicine, but is often limited by the low efficiency of DNA transfection. We previously reported that depletion of the autophagy receptor p62/SQSTM1 enhances DNA transfection …
View article: Gcn2 eIF2α kinase mediates combinatorial translational regulation through nucleotide motifs and uORFs in target mRNAs
Gcn2 eIF2α kinase mediates combinatorial translational regulation through nucleotide motifs and uORFs in target mRNAs Open
The protein kinase Gcn2 is a central transducer of nutritional stress signaling important for stress adaptation by normal cells and the survival of cancer cells. In response to nutrient deprivation, Gcn2 phosphorylates eIF2α, thereby repre…
View article: A Case of EGFR Gene Mutation-positive Early Lung Adenocarcinoma That Recurred 12 Years Postoperatively with Meningeal Carcinomatosis Alone
A Case of EGFR Gene Mutation-positive Early Lung Adenocarcinoma That Recurred 12 Years Postoperatively with Meningeal Carcinomatosis Alone Open
背景.髄膜癌腫症は肺癌の合併症の一つである.ただし多くの患者は髄膜以外に転移性病変を伴う.早期非小細胞肺癌では,手術後4年以降の再発率は低下する.我々は髄膜癌腫症単独で術後12年目に再発したepidermal growth factor receptor(EGFR)遺伝子変異陽性早期肺腺癌の1例を経験した.症例.77歳女性.物忘れと歩行困難があり,当院を受診した.脳造影MRI検査では異常がなかった.髄液細胞診で腺癌細胞を検出し,EGFR遺伝子変異(L858R)を認めた.髄膜…
View article: Regulation of ectopic heterochromatin-mediated epigenetic diversification by the JmjC family protein Epe1
Regulation of ectopic heterochromatin-mediated epigenetic diversification by the JmjC family protein Epe1 Open
H3K9 methylation (H3K9me) is a conserved marker of heterochromatin, a transcriptionally silent chromatin structure. Knowledge of the mechanisms for regulating heterochromatin distribution is limited. The fission yeast JmjC domain-containin…
View article: Roles of Nup133, Nup153 and membrane fenestrations in assembly of the nuclear pore complex at the end of mitosis
Roles of Nup133, Nup153 and membrane fenestrations in assembly of the nuclear pore complex at the end of mitosis Open
Reassembly of the nuclear pore complex (NPC) at the end of mitosis is an important event for eukaryotic nuclear function. In this study, we examined the dynamic behaviors of the endoplasmic reticulum (ER) by “Live CLEM” imaging. In metapha…
View article: Identification of the evolutionarily conserved nuclear envelope proteins Lem2 and MicLem2 in Tetrahymena thermophila
Identification of the evolutionarily conserved nuclear envelope proteins Lem2 and MicLem2 in Tetrahymena thermophila Open
Lem2 family proteins, i.e. the LAP2-Emerin-MAN1 (LEM) domain-containing nuclear envelope proteins, are well-conserved from yeasts to humans, both of which belong to the Opisthokonta supergroup. However, whether their homologs are present i…
View article: Nuclear localization signal targeting to macronucleus and micronucleus in binucleated ciliate <i>Tetrahymena thermophila</i>
Nuclear localization signal targeting to macronucleus and micronucleus in binucleated ciliate <i>Tetrahymena thermophila</i> Open
Ciliated protozoa possess two morphologically and functionally distinct nuclei: a macronucleus (MAC) and a micronucleus (MIC). The MAC is transcriptionally active and functions in all cellular events. The MIC is transcriptionally inactive …
View article: p62/<scp>SQSTM</scp>1 promotes rapid ubiquitin conjugation to target proteins after endosome rupture during xenophagy
p62/<span>SQSTM</span>1 promotes rapid ubiquitin conjugation to target proteins after endosome rupture during xenophagy Open
Autophagy is a bulk degradation pathway, and selective autophagy to remove foreign entities is called xenophagy. The conjugation of ubiquitin to target pathogens is an important process in xenophagy but when and where this ubiquitination o…
View article: Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliate <i>Tetrahymena</i>
Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliate <i>Tetrahymena</i> Open
The nuclear pore complex (NPC), a gateway for nucleocytoplasmic trafficking, is composed of ∼30 different proteins called nucleoporins. It remains unknown whether the NPCs within a species are homogeneous or vary depending on the cell type…
View article: Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in ciliate<i>Tetrahymena</i>
Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in ciliate<i>Tetrahymena</i> Open
SUMMARY STATEMENT Our study demonstrates compositional and structural differences of the nuclear pore complex between the functionally differentiated macronucleus and micronucleus within a single cytoplasm of ciliated protozoa. ABSTRACT Th…
View article: Inner nuclear membrane protein Lem2 augments heterochromatin formation in response to nutritional conditions
Inner nuclear membrane protein Lem2 augments heterochromatin formation in response to nutritional conditions Open
Inner nuclear membrane proteins interact with chromosomes in the nucleus and are important for chromosome activity. Lem2 and Man1 are conserved members of the LEM ‐domain nuclear membrane protein family. Mutations of LEM ‐domain proteins a…
View article: Depletion of autophagy receptor p62/<scp>SQSTM</scp>1 enhances the efficiency of gene delivery in mammalian cells
Depletion of autophagy receptor p62/<span>SQSTM</span>1 enhances the efficiency of gene delivery in mammalian cells Open
Novel methods that increase the efficiency of gene delivery to cells will have many useful applications. Here, we report a simple approach involving depletion of p62/ SQSTM 1 to enhance the efficiency of gene delivery. The efficiency of re…
View article: A non-inheritable maternal Cas9-based multiple-gene editing system in mice
A non-inheritable maternal Cas9-based multiple-gene editing system in mice Open
The CRISPR/Cas9 system is capable of editing multiple genes through one-step zygote injection. The preexisting method is largely based on the co-injection of Cas9 DNA (or mRNA) and guide RNAs (gRNAs); however, it is unclear how many genes …