Chie Tomikawa
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View article: tRNA<sup>Val</sup>allows four-way decoding with unmodified uridine at the wobble position in<i>Lactobacillus casei</i>
tRNA<sup>Val</sup>allows four-way decoding with unmodified uridine at the wobble position in<i>Lactobacillus casei</i> Open
Modifications at the wobble position (position 34) of tRNA facilitate interactions that enable or stabilize non-Watson–Crick base pairs. In bacterial tRNA, 5-hydroxyuridine (ho 5 U) derivatives xo 5 U [x: methyl (mo 5 U), carboxymethyl (cm…
View article: Recombinant expression and purification of phenylalanyl-tRNA synthetase from wheat: a long-lasting poly(U)-dependent poly(Phe) synthesis system
Recombinant expression and purification of phenylalanyl-tRNA synthetase from wheat: a long-lasting poly(U)-dependent poly(Phe) synthesis system Open
Synthetic genes for the two subunits of phenylalanyl-tRNA synthetase (PheRS) from wheat were expressed in Escherichia coli. When each gene was induced individually, the α subunit with a cleavable 6 × His tag at the amino terminus was large…
View article: Mechanism of tRNA recognition by heterotetrameric glycyl-tRNA synthetase from lactic acid bacteria
Mechanism of tRNA recognition by heterotetrameric glycyl-tRNA synthetase from lactic acid bacteria Open
Glycyl-tRNA synthetases (GlyRSs) have different oligomeric structures depending on the organisms. While a dimeric α2 GlyRS species is present in archaea, eukaryotes and some eubacteria, a heterotetrameric α2β2 GlyRS species is found in mos…
View article: Intron-Dependent or Independent Pseudouridylation of Precursor tRNA Containing Atypical Introns in Cyanidioschyzon merolae
Intron-Dependent or Independent Pseudouridylation of Precursor tRNA Containing Atypical Introns in Cyanidioschyzon merolae Open
Eukaryotic precursor tRNAs (pre-tRNAs) often have an intron between positions 37 and 38 of the anticodon loop. However, atypical introns are found in some eukaryotes and archaea. In an early-diverged red alga Cyanidioschyzon merolae, the t…
View article: Recognition of tRNA<sup>Ile</sup> with a UAU anticodon by isoleucyl‐tRNA synthetase in lactic acid bacteria
Recognition of tRNA<sup>Ile</sup> with a UAU anticodon by isoleucyl‐tRNA synthetase in lactic acid bacteria Open
In almost all eubacteria, the AUA codon is translated by tRNA Ile2 bearing lysidine at the wobble position. Lysidine is introduced by tRNA Ile lysidine synthetase (TilS) via post‐transcriptional modification of the cytidine of tRNA Ile2 (C…
View article: 7-Methylguanosine Modifications in Transfer RNA (tRNA)
7-Methylguanosine Modifications in Transfer RNA (tRNA) Open
More than 90 different modified nucleosides have been identified in tRNA. Among the tRNA modifications, the 7-methylguanosine (m7G) modification is found widely in eubacteria, eukaryotes, and a few archaea. In most cases, the m7G modificat…
View article: 7-Methylguanosine Modifications in tRNA
7-Methylguanosine Modifications in tRNA Open
More than 90 different modified nucleosides have been identified in tRNA. Among the tRNA modifications, the 7-methylguanosine (m7G) modification is found widely in eubacteria, eukaryotes, and a few archaea. In most cases, the m7G modificat…
View article: Transfer RNA Modification Enzymes from Thermophiles and Their Modified Nucleosides in tRNA
Transfer RNA Modification Enzymes from Thermophiles and Their Modified Nucleosides in tRNA Open
To date, numerous modified nucleosides in tRNA as well as tRNA modification enzymes have been identified not only in thermophiles but also in mesophiles. Because most modified nucleosides in tRNA from thermophiles are common to those in tR…
View article: Characterization of redundant tRNAIles with CAU and UAU anticodons in Lactobacillus plantarum
Characterization of redundant tRNAIles with CAU and UAU anticodons in Lactobacillus plantarum Open
In most eubacteria, the minor AUA isoleucine codon is decoded by tRNAIle2, which has a lysidine (L) in the anticodon loop. The lysidine is introduced by tRNAIle-lysidine synthetase (TilS) through post-transcriptional modification of cytidi…
View article: Long and branched polyamines are required for maintenance of the ribosome, tRNA<sup>His</sup> and tRNA<sup>Tyr</sup> in <i>Thermus thermophilus</i> cells at high temperatures
Long and branched polyamines are required for maintenance of the ribosome, tRNA<sup>His</sup> and tRNA<sup>Tyr</sup> in <i>Thermus thermophilus</i> cells at high temperatures Open
Thermus thermophilus is an extremely thermophilic eubacterium that produces various polyamines. Aminopropylagmatine ureohydrolase (SpeB) and SAM decarboxylase‐like protein 1 (SpeD1) are involved in the biosynthesis of spermidine from argin…