Chien-Wei Wang
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View article: Characterization of M11-like and OC125-like Monoclonal Antibody Binding to CA125 Tandem Repeats
Characterization of M11-like and OC125-like Monoclonal Antibody Binding to CA125 Tandem Repeats Open
The CA125 epitope within the MUC16 tandem repeat region is detected via the CA125 II test for ovarian cancer surveillance. This test utilizes the M11 and OC125 antibodies. A revised model of MUC16 with 19 tandem repeats has recently been i…
View article: Mapping the Binding Sites of CA125-Specific Antibodies on a Revised Molecular Model of MUC16
Mapping the Binding Sites of CA125-Specific Antibodies on a Revised Molecular Model of MUC16 Open
Background: The ovarian cancer biomarker CA125 is a peptide epitope found in multiple tandem repeat domains of the mucin MUC16. Although efforts have been undertaken to characterize the interaction between CA125 and its clinically used ant…
View article: Figure S9 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Figure S9 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Comparison of AlphaFold predicted MUC16 tandem repeat and murine SEA domain (PDB 1IVZ, reference 36). The repeat 6 structure predicted by AlphaFold (brown) and NMR solution structure of a murine SEA domain (gray) are overlayed. The SEA dom…
View article: Figure S6 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Figure S6 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Comparison of the sequence reported in this study to the sequence reported by O’Brien and co-workers (AF414442.2). Tandem repeats were aligned using Clustal Omega and visualized using Jalview with the Clustal Omega standard color scheme. T…
View article: Figure S3 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Figure S3 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Nanopore sequencing dot plots. The sequencing dot plots show the read length and average read quality. Each dot represents a single read. (A) Kuramochi, (B) OVCAR3, (C) OVCAR5, (D) OV1, (E) OV2, (F) OV3. Note that the axes for each plot ar…
View article: Figure S5 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Figure S5 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Clustal Omega alignment of the 19 tandem repeats. The tandem repeats were aligned using Clustal Omega and visualized using Jalview with the Clustal Omega standard color scheme. The consensus sequence shows abundance of each amino acid at e…
View article: Supplementary Document 3 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Supplementary Document 3 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Protein alignment
View article: FIGURE 4 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
FIGURE 4 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Schematic models of MUC16. Top, The model derived from the sequence determined by O'Brien and colleagues (2001) containing 63 units in the tandem repeat domain. Bottom, The model proposed in this study, containing 19 units in the tandem re…
View article: FIGURE 2 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
FIGURE 2 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Proteomic analysis of immunoprecipitated MUC16. Identified MUC16 peptides from immunoprecipitated (A) OVCAR3 cell lysate and (B) pooled ascites mapped onto the proposed repeat domains of MUC16. The repeat domains are each rep…
View article: Figure S2 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Figure S2 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Gel image of cDNA products from RT-PCR from patient tumors. (A) Lanes 1-3: NEB 1 kb Extend DNA Ladder, RT-PCR product of OV2 and OV3. Asterisk indicates the location of the approximate 10 kbp cDNA product. (B) Lane 1: NEB 1 kb DNA Ladder. …
View article: Figure S7 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Figure S7 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
AlphaFold predicted models of individual tandem repeats. (A)-(S) Repeats 1-19.
View article: TABLE 2 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
TABLE 2 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
The amino acid consensus sequence derived from long-read cDNA sequencing of six sources of MUC16 mRNA
View article: FIGURE 4 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
FIGURE 4 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Schematic models of MUC16. Top, The model derived from the sequence determined by O'Brien and colleagues (2001) containing 63 units in the tandem repeat domain. Bottom, The model proposed in this study, containing 19 units in the tandem re…
View article: Supplementary Document 1 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Supplementary Document 1 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
R script used for proteomics data analysis
View article: Supplementary Document 2 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Supplementary Document 2 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
DNA alignment
View article: Figure S2 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Figure S2 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Gel image of cDNA products from RT-PCR from patient tumors. (A) Lanes 1-3: NEB 1 kb Extend DNA Ladder, RT-PCR product of OV2 and OV3. Asterisk indicates the location of the approximate 10 kbp cDNA product. (B) Lane 1: NEB 1 kb DNA Ladder. …
View article: Supplementary Document 3 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Supplementary Document 3 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Protein alignment
View article: FIGURE 2 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
FIGURE 2 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Proteomic analysis of immunoprecipitated MUC16. Identified MUC16 peptides from immunoprecipitated (A) OVCAR3 cell lysate and (B) pooled ascites mapped onto the proposed repeat domains of MUC16. The repeat domains are each rep…
View article: Figure S4 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Figure S4 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Proteomic analysis of immunoaffinity-free enriched MUC16. Identified MUC16 peptides from ascites-derived MUC16 from three patients (A) Patient 1, (B) Patient 2, (C) Patient 3. The repeat domains are each represented by a horizontal line, a…
View article: TABLE 1 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
TABLE 1 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Primer sequences used for RT-PCR
View article: Figure S4 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Figure S4 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Proteomic analysis of immunoaffinity-free enriched MUC16. Identified MUC16 peptides from ascites-derived MUC16 from three patients (A) Patient 1, (B) Patient 2, (C) Patient 3. The repeat domains are each represented by a horizontal line, a…
View article: Supplementary Document 1 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Supplementary Document 1 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
R script used for proteomics data analysis
View article: Figure S6 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Figure S6 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Comparison of the sequence reported in this study to the sequence reported by O’Brien and co-workers (AF414442.2). Tandem repeats were aligned using Clustal Omega and visualized using Jalview with the Clustal Omega standard color scheme. T…
View article: TABLE 2 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
TABLE 2 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
The amino acid consensus sequence derived from long-read cDNA sequencing of six sources of MUC16 mRNA
View article: Figure S7 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Figure S7 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
AlphaFold predicted models of individual tandem repeats. (A)-(S) Repeats 1-19.
View article: Data from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Data from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
The biomarker CA125, a peptide epitope located in several tandem repeats of the mucin MUC16, is the gold standard for monitoring regression and recurrence of high-grade serous ovarian cancer in response to therapy. However, the CA125 epito…
View article: FIGURE 1 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
FIGURE 1 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Gel image of cDNA products from RT-PCR. Lane 1: NEB 1 kb Extend DNA Ladder. Lanes 2–4: RT-PCR product from Kuramochi, OVCAR3 and OVCAR5 cells, respectively. The asterisk indicates the location of the approximate 10 kbp cDNA product.
View article: FIGURE 6 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
FIGURE 6 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
AlphaFold predicted MUC16 tandem repeat model. The 19 tandem repeat structures were predicted individually and overlaid. All repeats contain a similar structure including two alpha helices, one beta sheet, and a proline/serine/threonine-ri…
View article: Supplementary Document 4 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Supplementary Document 4 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Mutation table
View article: Figure S8 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Figure S8 from A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing Open
Comparison of AlphaFold and I-TASSER predicted MUC16 tandem repeat model. The repeat 6 structure predicted by AlphaFold (brown) and I-TASSER (blue) are overlayed. The SEA domains predicted by the two algorithms show high similarity.