Chi‐Ling Chiang
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View article: Extracellular Vesicular Delta‐Like Ligand 3 and Subtype Transcription Factors for Small Cell Lung Cancer Diagnosis
Extracellular Vesicular Delta‐Like Ligand 3 and Subtype Transcription Factors for Small Cell Lung Cancer Diagnosis Open
Small cell lung cancer (SCLC) is associated with high mortality and limited therapeutic options. There is increasing recognition that SCLC harbors molecular heterogeneity. Using a new liquid biopsy assay, it is demonstrated that SCLC subty…
View article: Integrated Antigenic and Nucleic Acid Detection in Single Virions and Extracellular Vesicles with Viral Content
Integrated Antigenic and Nucleic Acid Detection in Single Virions and Extracellular Vesicles with Viral Content Open
Virion‐mediated outbreaks are imminent and despite rapid responses, continue to cause adverse symptoms and death. Therefore, tunable, sensitive, high‐throughput assays are needed to help diagnose future virion‐mediated outbreaks. Herein, i…
View article: Engineered extracellular vesicles carrying let-7a-5p for alleviating inflammation in acute lung injury
Engineered extracellular vesicles carrying let-7a-5p for alleviating inflammation in acute lung injury Open
Background Acute lung injury (ALI) is a life-threatening respiratory condition characterized by severe inflammation and lung tissue damage, frequently causing rapid respiratory failure and long-term complications. The microRNA let-7a-5p is…
View article: Extracellular Vesicular Analysis of Glypican 1 mRNA and Protein for Pancreatic Cancer Diagnosis and Prognosis
Extracellular Vesicular Analysis of Glypican 1 mRNA and Protein for Pancreatic Cancer Diagnosis and Prognosis Open
Detecting pancreatic duct adenocarcinoma (PDAC) in its early stages and predicting late‐stage patient prognosis undergoing chemotherapy is challenging. This work shows that the activation of specific oncogenes leads to elevated expression …
View article: Dual targeted extracellular vesicles regulate oncogenic genes in advanced pancreatic cancer
Dual targeted extracellular vesicles regulate oncogenic genes in advanced pancreatic cancer Open
Pancreatic ductal adenocarcinoma (PDAC) tumours carry multiple gene mutations and respond poorly to treatments. There is currently an unmet need for drug carriers that can deliver multiple gene cargoes to target high solid tumour burden li…
View article: Exosomal mRNAs for Angiogenic–Osteogenic Coupled Bone Repair
Exosomal mRNAs for Angiogenic–Osteogenic Coupled Bone Repair Open
Regenerative medicine in tissue engineering often relies on stem cells and specific growth factors at a supraphysiological dose. These approaches are costly and may cause severe side effects. Herein, therapeutic small extracellular vesicle…
View article: Integrated antigenic and nucleic acid detection in single virions and virion-infected host-derived extracellular vesicles
Integrated antigenic and nucleic acid detection in single virions and virion-infected host-derived extracellular vesicles Open
Virion-mediated outbreaks are imminent and despite rapid responses, they continue to cause adverse symptoms and death. Therefore, tunable, sensitive, high-throughput assays are needed to control future virion-mediated outbreaks. Herein, we…
View article: Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy
Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy Open
View article: 182P Cancer vaccines based on whole-tumor-lysate or neoepitopes with validated HLA-binding outperform those with predicted HLA-binding affinity
182P Cancer vaccines based on whole-tumor-lysate or neoepitopes with validated HLA-binding outperform those with predicted HLA-binding affinity Open
Vaccines represent attractive components of the tumor immunotherapy armamentarium. Although whole tumor-based vaccines and private neoantigens-based vaccines; each have pros and cons, their relative efficacy has never been comprehensively …
View article: An immunogold single extracellular vesicular RNA and protein ( <sup>Au</sup> SERP) biochip to predict responses to immunotherapy in non‐small cell lung cancer patients
An immunogold single extracellular vesicular RNA and protein ( <sup>Au</sup> SERP) biochip to predict responses to immunotherapy in non‐small cell lung cancer patients Open
Conventional PD‐L1 immunohistochemical tissue biopsies only predict 20%–40% of non‐small cell lung cancer (NSCLC) patients that will respond positively to anti‐PD‐1/PD‐L1 immunotherapy. Herein, we present an immunogold biochip to quantify …
View article: Leukemia-initiating HSCs in chronic lymphocytic leukemia reveal clonal leukemogenesis and differential drug sensitivity
Leukemia-initiating HSCs in chronic lymphocytic leukemia reveal clonal leukemogenesis and differential drug sensitivity Open
The existence of "leukemia-initiating cells" (LICs) in chronic lymphocytic leukemia (CLL) remains controversial due to the difficulty in isolating and identifying the tumor-initiating cells. Here, we demonstrate a microchannel electroporat…
View article: ROR1 targeted immunoliposomal delivery of OSU-2S shows selective cytotoxicity in t(1;19)(q23;p13) translocated B-cell acute lymphoblastic leukemia
ROR1 targeted immunoliposomal delivery of OSU-2S shows selective cytotoxicity in t(1;19)(q23;p13) translocated B-cell acute lymphoblastic leukemia Open
View article: Microfluidic harvesting of breast cancer tumor spheroid-derived extracellular vesicles from immobilized microgels for single-vesicle analysis
Microfluidic harvesting of breast cancer tumor spheroid-derived extracellular vesicles from immobilized microgels for single-vesicle analysis Open
A novel microfluidic system for the low-volume harvesting of extracellular vesicles from breast cancer tumor spheroids encapsulated within immobilized hydrogel microbioreactors for downstream single-vesicle analyses.
View article: PP2A is a therapeutically targetable driver of cell fate decisions via a c-Myc/p21 axis in human and murine acute myeloid leukemia
PP2A is a therapeutically targetable driver of cell fate decisions via a c-Myc/p21 axis in human and murine acute myeloid leukemia Open
Dysregulated cellular differentiation is a hallmark of acute leukemogenesis. Phosphatases are widely suppressed in cancers but have not been traditionally associated with differentiation. In this study, we found that the silencing of prote…
View article: The ROR1 antibody-drug conjugate huXBR1-402-G5-PNU effectively targets ROR1+ leukemia
The ROR1 antibody-drug conjugate huXBR1-402-G5-PNU effectively targets ROR1+ leukemia Open
Antibody-drug conjugates directed against tumor-specific targets have allowed targeted delivery of highly potent chemotherapy to malignant cells while sparing normal cells. Receptor tyrosine kinase-like orphan receptor 1 (ROR1) is an oncof…
View article: From Nanoparticles to Cancer Nanomedicine: Old Problems with New Solutions
From Nanoparticles to Cancer Nanomedicine: Old Problems with New Solutions Open
Anticancer nanomedicines have been studied over 30 years, but fewer than 10 formulations have been approved for clinical therapy today. Despite abundant options of anticancer drugs, it remains challenging to have agents specifically target…
View article: Author Correction: Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation
Author Correction: Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation Open
View article: LC-FACSeq is a method for detecting rare clones in leukemia
LC-FACSeq is a method for detecting rare clones in leukemia Open
Detecting, characterizing, and monitoring rare populations of cells can increase testing sensitivity, give insight into disease mechanism, and inform clinical decision making. One area that can benefit from increased resolution is manageme…
View article: CLEAR: coverage-based limiting-cell experiment analysis for RNA-seq
CLEAR: coverage-based limiting-cell experiment analysis for RNA-seq Open
View article: Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation
Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation Open
View article: ROR1-targeted delivery of miR-29b induces cell cycle arrest and therapeutic benefit in vivo in a CLL mouse model
ROR1-targeted delivery of miR-29b induces cell cycle arrest and therapeutic benefit in vivo in a CLL mouse model Open
Chronic lymphocytic leukemia (CLL) occurs in 2 major forms: aggressive and indolent. Low miR-29b expression in aggressive CLL is associated with poor prognosis. Indiscriminate miR-29b overexpression in the B-lineage of mice causes aberranc…
View article: CLEAR: Coverage-based Limiting-cell Experiment Analysis for RNA-seq
CLEAR: Coverage-based Limiting-cell Experiment Analysis for RNA-seq Open
Direct cDNA preamplification protocols developed for single-cell RNA-seq have enabled transcriptome profiling of precious clinical samples and rare cells without sample pooling or RNA extraction. Currently, there is no algorithm optimized …
View article: Nanofabrication: Controllable Large-Scale Transfection of Primary Mammalian Cardiomyocytes on a Nanochannel Array Platform (Small 43/2016)
Nanofabrication: Controllable Large-Scale Transfection of Primary Mammalian Cardiomyocytes on a Nanochannel Array Platform (Small 43/2016) Open
On page 5971, D. Gallego-Perez, W. Lu, L. J. Lee, and co-workers report an advanced Bio-NEMS (nanoelectromechanical system) device for high-throughput cell trapping and gene delivery on a silicon-nanochannel array chip. With a thin-film hy…
View article: Controllable Large-Scale Transfection of Primary Mammalian Cardiomyocytes on a Nanochannel Array Platform
Controllable Large-Scale Transfection of Primary Mammalian Cardiomyocytes on a Nanochannel Array Platform Open
While electroporation has been widely used as a physical method for gene transfection in vitro and in vivo, its application in gene therapy of cardiovascular cells remains challenging. Due to the high concentration of ion-transport protein…
View article: Targeting the RAS/MAPK pathway with<i>miR-181a</i>in acute myeloid leukemia
Targeting the RAS/MAPK pathway with<i>miR-181a</i>in acute myeloid leukemia Open
Deregulation of microRNAs' expression frequently occurs in acute myeloid leukemia (AML). Lower miR-181a expression is associated with worse outcomes, but the exact mechanisms by which miR-181a mediates this effect remain elusive. Aberrant …
View article: Pharmacological activation of lysophosphatidic acid receptors regulates erythropoiesis
Pharmacological activation of lysophosphatidic acid receptors regulates erythropoiesis Open
Lysophosphatidic acid (LPA), a growth factor-like phospholipid, regulates numerous physiological functions, including cell proliferation and differentiation. In a previous study, we have demonstrated that LPA activates erythropoiesis by ac…
View article: Induced Apoptosis Investigation in Wild-type and FLT3-ITD Acute Myeloid Leukemia Cells by Nanochannel Electroporation and Single-cell qRT-PCR
Induced Apoptosis Investigation in Wild-type and FLT3-ITD Acute Myeloid Leukemia Cells by Nanochannel Electroporation and Single-cell qRT-PCR Open
Nanochannel electroporation (NEP) was applied to deliver precise dosages of myeloid cell leukemia-1 (Mcl-1)-specific siRNA and molecular beacons to two types of acute myeloid leukemia (AML) cells, FMS-like tyrosine kinase-3 wild-type (WT) …
View article: Bosch etching for the creation of a 3D nanoelectroporation system for high throughput gene delivery
Bosch etching for the creation of a 3D nanoelectroporation system for high throughput gene delivery Open
In order to create a high-throughput electroporation based cell transfection system, it is required that each cell has localized delivery and minimal membrane damage to ensure optimal transfection and longevity post-biomolecule delivery. T…
View article: ROR1-targeted delivery of OSU-2S, a nonimmunosuppressive FTY720 derivative, exerts potent cytotoxicity in mantle-cell lymphoma in vitro and in vivo
ROR1-targeted delivery of OSU-2S, a nonimmunosuppressive FTY720 derivative, exerts potent cytotoxicity in mantle-cell lymphoma in vitro and in vivo Open
View article: A Novel 96well-formatted Micro-gap Plate Enabling Drug Response Profiling on Primary Tumour Samples
A Novel 96well-formatted Micro-gap Plate Enabling Drug Response Profiling on Primary Tumour Samples Open
Drug-based treatments are the most widely used interventions for cancer management. Personalized drug response profiling remains inherently challenging with low cell count harvested from tumour sample. We present a 96well-formatted microfl…