F. Chris H. Franklin
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View article: SCEP3 initiates synapsis and implements crossover interference in Arabidopsis
SCEP3 initiates synapsis and implements crossover interference in Arabidopsis Open
The synaptonemal complex (SC) is a meiosis-specific tripartite proteinaceous structure that regulates the number and positions of crossovers (COs). Here we characterize SCEP3, a new Arabidopsis SC component that is essential for CO assuran…
View article: Heat Stress, Starvation, and Heat Stress Plus Starvation Cause Unique Transcriptomic Responses in the Economically Important Red Abalone Haliotis rufescens
Heat Stress, Starvation, and Heat Stress Plus Starvation Cause Unique Transcriptomic Responses in the Economically Important Red Abalone Haliotis rufescens Open
Although most marine invertebrates are experiencing multiple environmental stressors simultaneously, the transcriptome-wide gene expression responses to multiple stressors remain understudied. We used RNA-sequencing to assess the transcrip…
View article: <scp>FIGL1</scp> prevents aberrant chromosome associations and fragmentation and limits crossovers in polyploid wheat meiosis
<span>FIGL1</span> prevents aberrant chromosome associations and fragmentation and limits crossovers in polyploid wheat meiosis Open
Summary Meiotic crossovers (COs) generate genetic diversity and are crucial for viable gamete production. Plant COs are typically limited to 1–3 per chromosome pair, constraining the development of improved varieties, which in wheat is exa…
View article: Identification, characterization, and rescue of <scp>CRISPR</scp>/Cas9 generated wheat <i>SPO11‐1</i> mutants
Identification, characterization, and rescue of <span>CRISPR</span>/Cas9 generated wheat <i>SPO11‐1</i> mutants Open
Summary Increasing crop yields through plant breeding is time consuming and laborious, with the generation of novel combinations of alleles being limited by chromosomal linkage blocks and linkage‐drag. Meiotic recombination is essential to…
View article: Unravelling mechanisms that govern meiotic crossover formation in wheat
Unravelling mechanisms that govern meiotic crossover formation in wheat Open
Wheat is a major cereal crop that possesses a large allopolyploid genome formed through hybridisation of tetraploid and diploid progenitors. During meiosis, crossovers (COs) are constrained in number to 1–3 per chromosome pair that are pre…
View article: Differentiated function and localisation of SPO11-1 and PRD3 on the chromosome axis during meiotic DSB formation in Arabidopsis thaliana
Differentiated function and localisation of SPO11-1 and PRD3 on the chromosome axis during meiotic DSB formation in Arabidopsis thaliana Open
During meiosis, DNA double-strand breaks (DSBs) occur throughout the genome, a subset of which are repaired to form reciprocal crossovers between chromosomes. Crossovers are essential to ensure balanced chromosome segregation and to create…
View article: The Role of Topoisomerase II in DNA Repair and Recombination in Arabidopsis thaliana
The Role of Topoisomerase II in DNA Repair and Recombination in Arabidopsis thaliana Open
DNA entanglements and supercoiling arise frequently during normal DNA metabolism. DNA topoisomerases are highly conserved enzymes that resolve the topological problems that these structures create. Topoisomerase II (TOPII) releases topolog…
View article: Crossover-active regions of the wheat genome are distinguished by DMC1, the chromosome axis, H3K27me3, and signatures of adaptation
Crossover-active regions of the wheat genome are distinguished by DMC1, the chromosome axis, H3K27me3, and signatures of adaptation Open
The hexaploid bread wheat genome comprises over 16 gigabases of sequence across 21 chromosomes. Meiotic crossovers are highly polarized along the chromosomes, with elevation in the gene-dense distal regions and suppression in the Gypsy ret…
View article: Arabidopsis meiotic crossover hot spots overlap with H2A.Z nucleosomes at gene promoters
Arabidopsis meiotic crossover hot spots overlap with H2A.Z nucleosomes at gene promoters Open
PRDM9 directs human meiotic crossover hotspots to intergenic sequence motifs, whereas budding yeast hotspots overlap low nucleosome density regions in gene promoters. To investigate hotspots in plants, which lack PRDM9, we used coalescent …
View article: ZYP1 is required for obligate cross-over formation and cross-over interference in<i>Arabidopsis</i>
ZYP1 is required for obligate cross-over formation and cross-over interference in<i>Arabidopsis</i> Open
Significance The synaptonemal complex (SC) is a meiosis-specific proteinaceous ultrastructure required to ensure cross-over (CO) formation in the majority of sexually reproducing eukaryotes. It is composed of two lateral elements adjoined …
View article: Distal Bias of Meiotic Crossovers in Hexaploid Bread Wheat Reflects Spatio-Temporal Asymmetry of the Meiotic Program
Distal Bias of Meiotic Crossovers in Hexaploid Bread Wheat Reflects Spatio-Temporal Asymmetry of the Meiotic Program Open
Meiotic recombination generates genetic variation and provides physical links between homologous chromosomes (crossovers) essential for accurate segregation. In cereals the distribution of crossovers, cytologically evident as chiasmata, is…
View article: Meiotic chromosome axis remodelling is critical for meiotic recombination in<i>Brassica rapa</i>
Meiotic chromosome axis remodelling is critical for meiotic recombination in<i>Brassica rapa</i> Open
Meiosis generates genetic variation through homologous recombination (HR) that is harnessed during breeding. HR occurs in the context of meiotic chromosome axes and the synaptonemal complex. To study the role of axis remodelling in crossov…
View article: Arabidopsis PCH2 Mediates Meiotic Chromosome Remodeling and Maturation of Crossovers
Arabidopsis PCH2 Mediates Meiotic Chromosome Remodeling and Maturation of Crossovers Open
Meiotic chromosomes are organized into linear looped chromatin arrays by a protein axis localized along the loop-bases. Programmed remodelling of the axis occurs during prophase I of meiosis. Structured illumination microscopy (SIM) has re…
View article: Derived alleles of two axis proteins affect meiotic traits in autotetraploid <i>Arabidopsis arenosa</i>
Derived alleles of two axis proteins affect meiotic traits in autotetraploid <i>Arabidopsis arenosa</i> Open
Polyploidy, which results from whole genome duplication (WGD), has shaped the long-term evolution of eukaryotic genomes in all kingdoms. Polyploidy is also implicated in adaptation, domestication, and speciation. Yet when WGD newly occurs,…
View article: Interacting Genomic Landscapes of REC8-Cohesin, Chromatin, and Meiotic Recombination in Arabidopsis
Interacting Genomic Landscapes of REC8-Cohesin, Chromatin, and Meiotic Recombination in Arabidopsis Open
Meiosis recombines genetic variation and influences eukaryote genome evolution. During meiosis, DNA double-strand breaks (DSBs) enter interhomolog repair to yield crossovers and noncrossovers. DSB repair occurs as replicated sister chromat…
View article: The H3 histone chaperone NASP<sup>SIM3</sup> escorts CenH3 in Arabidopsis
The H3 histone chaperone NASP<sup>SIM3</sup> escorts CenH3 in Arabidopsis Open
Summary Centromeres define the chromosomal position where kinetochores form to link the chromosome to microtubules during mitosis and meiosis. Centromere identity is determined by incorporation of a specific histone H3 variant termed CenH3…
View article: REC8-cohesin, chromatin and transcription orchestrate meiotic recombination in the Arabidopsis genome
REC8-cohesin, chromatin and transcription orchestrate meiotic recombination in the Arabidopsis genome Open
During meiosis chromosomes undergo DNA double-strand breaks (DSBs) that can be repaired using a homolog to produce crossovers, which creates genetic diversity. Meiotic recombination occurs coincident with homolog pairing and polymerization…
View article: TOPII and chromosome movement help remove interlocks between entangled chromosomes during meiosis
TOPII and chromosome movement help remove interlocks between entangled chromosomes during meiosis Open
During the zygotene stage of meiosis, normal progression of chromosome synapsis and homologous recombination frequently lead to the formation of structural interlocks between entangled chromosomes. The persistence of interlocks through to …
View article: Identification of ASYNAPTIC4, a Component of the Meiotic Chromosome Axis
Identification of ASYNAPTIC4, a Component of the Meiotic Chromosome Axis Open
During the leptotene stage of prophase I of meiosis, chromatids become organized into a linear looped array via a protein axis that forms along the loop bases. Establishment of the axis is essential for the subsequent synapsis of the homol…
View article: Plasticity of Meiotic Recombination Rates in Response to Temperature in<i>Arabidopsis</i>
Plasticity of Meiotic Recombination Rates in Response to Temperature in<i>Arabidopsis</i> Open
Meiosis, the specialized cell division that generates gametes, shuffles parental genomes through homologous recombination. It was reported in Drosophila a century ago, that the recombination rate is sensitive to temperature, but how... Mei…
View article: Affinity proteomics reveals extensive phosphorylation of the Brassica chromosome axis protein <scp>ASY</scp>1 and a network of associated proteins at prophase I of meiosis
Affinity proteomics reveals extensive phosphorylation of the Brassica chromosome axis protein <span>ASY</span>1 and a network of associated proteins at prophase I of meiosis Open
Summary During meiosis, the formation of crossovers ( CO s) generates genetic variation and provides physical links that are essential for accurate chromosome segregation. CO s occur in the context of a proteinaceous chromosome axis. The t…
View article: MAP Kinase PrMPK9-1 Contributes to the Self-Incompatibility Response
MAP Kinase PrMPK9-1 Contributes to the Self-Incompatibility Response Open
Mitogen-activated protein kinases (MAPKs) form important signaling modules for a variety of cellular responses in eukaryotic cells. In plants, MAPKs play key roles in growth and development as well as in immunity/stress responses. Pollen-p…
View article: Natural variation and dosage of the HEI10 meiotic E3 ligase control <i>Arabidopsis</i> crossover recombination
Natural variation and dosage of the HEI10 meiotic E3 ligase control <i>Arabidopsis</i> crossover recombination Open
During meiosis, homologous chromosomes undergo crossover recombination, which creates genetic diversity and balances homolog segregation. Despite these critical functions, crossover frequency varies extensively within and between species. …
View article: Identification of Phosphorylation Sites Altering Pollen Soluble Inorganic Pyrophosphatase Activity
Identification of Phosphorylation Sites Altering Pollen Soluble Inorganic Pyrophosphatase Activity Open
Protein phosphorylation regulates numerous cellular processes. Identifying the substrates and protein kinases involved is vital to understand how these important posttranslational modifications modulate biological function in eukaryotic ce…
View article: Understanding and Manipulating Meiotic Recombination in Plants
Understanding and Manipulating Meiotic Recombination in Plants Open
Meiosis is a specialized cell division, essential in most reproducing organisms to halve the number of chromosomes, thereby enabling the restoration of ploidy levels during fertilization. A key step of meiosis is homologous recombination, …
View article: A spontaneous mutation in MutL‐Homolog 3 (Hv<scp>MLH</scp>3) affects synapsis and crossover resolution in the barley desynaptic mutant <i>des10</i>
A spontaneous mutation in MutL‐Homolog 3 (Hv<span>MLH</span>3) affects synapsis and crossover resolution in the barley desynaptic mutant <i>des10</i> Open
Summary Although meiosis is evolutionarily conserved, many of the underlying mechanisms show species‐specific differences. These are poorly understood in large genome plant species such as barley ( Hordeum vulgare ) where meiotic recombina…