Chris Oubridge
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View article: psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation
psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation Open
RNA helicases remodel the spliceosome to enable pre-mRNA splicing, but their binding and mechanism of action remain poorly understood. To define helicase-RNA contacts in specific spliceosomal states, we develop purified spliceosome iCLIP (…
View article: PsiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation
PsiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation Open
Eight RNA helicases remodel the spliceosome to effect pre-mRNA splicing but their mechanism of action remains poorly understood. We have developed “purified spliceosome iCLIP” (psiCLIP) to define helicase-RNA contacts in specific spliceoso…
View article: A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation
A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation Open
A human P spliceosome structure Splicing of some pre–messenger RNAs could be regulated by cell type–specific splicing factors. Fica et al. describe the cryo–electron microscopy structure of the human postcatalytic (P) spliceosome. Surprisi…
View article: Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p-binding domain
Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p-binding domain Open
Spliceosomal proteins Hsh49p and Cus1p are components of SF3b, which together with SF3a, Msl1p/Lea1p, Sm proteins, and U2 snRNA, form U2 snRNP, which plays a crucial role in pre-mRNA splicing. Hsh49p, comprising two RRMs, forms a heterodim…