Chris Preece
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View article: Optimizing approaches for targeted integration of transgenic cassettes by integrase-mediated cassette exchange in mouse and human stem cells
Optimizing approaches for targeted integration of transgenic cassettes by integrase-mediated cassette exchange in mouse and human stem cells Open
To enable robust expression of transgenes in stem cells, recombinase-mediated cassette exchange at safe harbor loci is frequently adopted. The choice of recombinase enzyme is a critical parameter to ensure maximum efficiency and accuracy o…
View article: Naturally sterile Mus spretus hybrids are suitable for the generation of pseudopregnant embryo transfer recipients
Naturally sterile Mus spretus hybrids are suitable for the generation of pseudopregnant embryo transfer recipients Open
For the preparation of embryo transfer recipients, surgically vasectomized mice are commonly used, generated by procedures associated with pain and discomfort. Sterile transgenic strains provide a nonsurgical replacement, but their mainten…
View article: Shieldin and CST co-orchestrate DNA polymerase-dependent tailed-end joining reactions independently of 53BP1-governed repair pathway choice
Shieldin and CST co-orchestrate DNA polymerase-dependent tailed-end joining reactions independently of 53BP1-governed repair pathway choice Open
53BP1 regulates DNA end-joining in lymphocytes, diversifying immune antigen receptors. This involves nucleosome-bound 53BP1 at DNA double-stranded breaks (DSBs) recruiting RIF1 and shieldin, a poorly understood DNA-binding complex. The 53B…
View article: Characterization of meiotic recombination intermediates through gene knockouts in founder hybrid mice
Characterization of meiotic recombination intermediates through gene knockouts in founder hybrid mice Open
Mammalian meiotic recombination proceeds via repair of hundreds of programmed DNA double-strand breaks, which requires choreographed binding of RPA, DMC1, and RAD51 to single-stranded DNA substrates. High-resolution in vivo binding maps of…
View article: Altering the Binding Properties of PRDM9 Partially Restores Fertility across the Species Boundary
Altering the Binding Properties of PRDM9 Partially Restores Fertility across the Species Boundary Open
Sterility or subfertility of male hybrid offspring is commonly observed. This phenomenon contributes to reproductive barriers between the parental populations, an early step in the process of speciation. One frequent cause of such infertil…
View article: A functional overlap between actively transcribed genes and chromatin boundary elements
A functional overlap between actively transcribed genes and chromatin boundary elements Open
Mammalian genomes are subdivided into large (50-2000 kb) regions of chromatin referred to as Topologically Associating Domains (TADs or sub-TADs). Chromatin within an individual TAD contacts itself more frequently than with regions in surr…
View article: Mechanics of mouse blastocyst hatching revealed by a hydrogel-based microdeformation assay
Mechanics of mouse blastocyst hatching revealed by a hydrogel-based microdeformation assay Open
Significance Blastocyst hatching is crucial for implantation of mammalian embryos and a common failure point during in vitro fertilization (IVF). We have little knowledge of the mechanical basis whereby an embryo hatches out of the zona pe…
View article: An Intronic <i>Flk1</i> Enhancer Directs Arterial-Specific Expression via RBPJ-Mediated Venous Repression
An Intronic <i>Flk1</i> Enhancer Directs Arterial-Specific Expression via RBPJ-Mediated Venous Repression Open
Objective— The vascular endothelial growth factor (VEGF) receptor Flk1 is essential for vascular development, but the signaling and transcriptional pathways by which its expression is regulated in endothelial cells remain unclear. Although…