Christian S. Lentz
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View article: Identification of covalent inhibitors of Staphylococcus aureus serine hydrolases important for virulence and biofilm formation
Identification of covalent inhibitors of Staphylococcus aureus serine hydrolases important for virulence and biofilm formation Open
Staphylococcus aureus is a leading cause of bacteria-associated mortality worldwide. New tools are needed to both image and treat this pathogen. We previously identified a group of S. aureus serine hydrolases (Fphs), which regulate aspects…
View article: Identification of multiple serine hydrolases involved in virulence and cell envelope integrity of Klebsiella pneumoniae
Identification of multiple serine hydrolases involved in virulence and cell envelope integrity of Klebsiella pneumoniae Open
Klebsiella pneumoniae is a resident of the human gastro-intestinal tract and an opportunistic, critical priority pathogen that can cause severe systemic infections. To overcome emerging multi-drug resistance, discovery and validation of no…
View article: Activity-Based Protein Profiling Identifies an α-Amylase Family Protein Contributing to the Virulence of Methicillin-Resistant <i>Staphylococcus aureus</i>
Activity-Based Protein Profiling Identifies an α-Amylase Family Protein Contributing to the Virulence of Methicillin-Resistant <i>Staphylococcus aureus</i> Open
In search of new putative antimicrobial drug targets in methicillin-resistant Staphylococcus aureus, we aimed to identify and characterize retaining glycosidase activities in this bacterial pathogen. Using activity-based protein profiling …
View article: A programmable, selection-free CRISPR interference system in<i>Staphylococcus aureus</i>
A programmable, selection-free CRISPR interference system in<i>Staphylococcus aureus</i> Open
Common dCas9-based CRISPR interference (CRISPRi) system for gene regulation requires antibiotic selection and exogenous inducer molecules, posing significant challenges when applied in in vivo bacterial infection models. Using Staphylococc…
View article: Single-cell phenotypic profiling and backtracing exposes and predicts clinically relevant subpopulations in isogenic Staphylococcus aureus communities
Single-cell phenotypic profiling and backtracing exposes and predicts clinically relevant subpopulations in isogenic Staphylococcus aureus communities Open
Isogenic bacterial cell populations are phenotypically heterogenous and may include subpopulations of antibiotic tolerant or heteroresistant cells. The reversibility of these phenotypes and lack of biomarkers to differentiate functionally …
View article: Development of an inducer-free, virulence gene promoter-controlled, and fluorescent reporter-labeled CRISPR interference system in <i>Staphylococcus aureus</i>
Development of an inducer-free, virulence gene promoter-controlled, and fluorescent reporter-labeled CRISPR interference system in <i>Staphylococcus aureus</i> Open
The dCas9-based Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) interference (CRISPRi) gene regulation technique requires two components: a catalytically inactive Cas9 protein (dCas9) and a single-guide RNA that targets …
View article: Activity-Based Protein Profiling Identifies<i>Klebsiella pneumoniae</i>Serine Hydrolases with Potential Roles in Host-Pathogen Interactions
Activity-Based Protein Profiling Identifies<i>Klebsiella pneumoniae</i>Serine Hydrolases with Potential Roles in Host-Pathogen Interactions Open
Klebsiella pneumoniae is a normal resident of the human gastro-intestinal tract and an opportunistic, critical priority pathogen that can cause a variety of severe systemic infections. Due to emerging multi-drug resistance of this pathogen…
View article: Deciphering <i>Staphylococcus aureus–</i> host dynamics using dual activity-based protein profiling of ATP-interacting proteins
Deciphering <i>Staphylococcus aureus–</i> host dynamics using dual activity-based protein profiling of ATP-interacting proteins Open
The utilization of ATP within cells plays a fundamental role in cellular processes that are essential for the regulation of host–pathogen dynamics and the subsequent immune response. This study focuses on ATP-binding proteins to dissect th…
View article: Comprehensive virulence profiling and evolutionary analysis of specificity determinants in <i>Staphylococcus aureus</i> two-component systems
Comprehensive virulence profiling and evolutionary analysis of specificity determinants in <i>Staphylococcus aureus</i> two-component systems Open
In the Staphylococcus aureus genome, a set of highly conserved two-component systems (TCSs) composed of histidine kinases (HKs) and their cognate response regulators (RRs) sense and respond to environmental stimuli, which drive the adaptat…
View article: Deciphering<i>Staphylococcus aureus-</i>host dynamics using dual activity-based protein profiling of ATP-interacting proteins
Deciphering<i>Staphylococcus aureus-</i>host dynamics using dual activity-based protein profiling of ATP-interacting proteins Open
The utilization of ATP within cells plays a fundamental role in cellular processes that are essential for the regulation of host-pathogen dynamics and the subsequent immune response. This study focuses on ATP-binding proteins to dissect th…
View article: Activity-based protein profiling of serine hydrolases and penicillin-binding proteins in <i>Enterococcus faecium</i>
Activity-based protein profiling of serine hydrolases and penicillin-binding proteins in <i>Enterococcus faecium</i> Open
Enterococcus faecium is a gut commensal bacterium which is gaining increasing relevance as an opportunistic, nosocomial pathogen. Its high level of intrinsic and acquired antimicrobial resistance is causing a lack of treatment options, par…
View article: High-throughput single-cell phenotypic profiling and backtracing exposes and predicts clinically relevant subpopulations in isogenic<i>Staphylococcus aureus</i>communities
High-throughput single-cell phenotypic profiling and backtracing exposes and predicts clinically relevant subpopulations in isogenic<i>Staphylococcus aureus</i>communities Open
Isogenic bacterial cell populations are phenotypically heterogenous and may include subpopulations of antibiotic tolerant or heteroresistant cells. The reversible nature of these phenotypes and lack of biomarkers to differentiate functiona…
View article: Comprehensive Virulence Profiling and Evolutionary Analysis of Specificity Determinants in<i>Staphylococcus aureus</i>Two-Component Systems
Comprehensive Virulence Profiling and Evolutionary Analysis of Specificity Determinants in<i>Staphylococcus aureus</i>Two-Component Systems Open
In the Staphylococcus aureus genome, a set of highly conserved two-component systems (TCSs) composed of histidine kinases (HKs) with their cognate response regulators (RRs) sense and respond to environmental stimuli, which drive the adapta…
View article: Biochemical and Cellular Characterization of the Function of Fluorophosphonate-Binding Hydrolase H (FphH) in <i>Staphylococcus aureus</i> Support a Role in Bacterial Stress Response
Biochemical and Cellular Characterization of the Function of Fluorophosphonate-Binding Hydrolase H (FphH) in <i>Staphylococcus aureus</i> Support a Role in Bacterial Stress Response Open
The development of new treatment options for bacterial infections requires access to new targets for antibiotics and antivirulence strategies. Chemoproteomic approaches are powerful tools for profiling and identifying novel druggable targe…
View article: Activity‐Based Protein Profiling in Methicillin‐Resistant <i>Staphylococcus aureus</i> Reveals the Broad Reactivity of a Carmofur‐Derived Probe
Activity‐Based Protein Profiling in Methicillin‐Resistant <i>Staphylococcus aureus</i> Reveals the Broad Reactivity of a Carmofur‐Derived Probe Open
Activity‐based protein profiling is a powerful chemoproteomic technique to detect active enzymes and identify targets and off‐targets of drugs. Here, we report the use of carmofur‐ and activity‐based probes to identify biologically relevan…
View article: From Differential Stains to Next Generation Physiology: Chemical Probes to Visualize Bacterial Cell Structure and Physiology
From Differential Stains to Next Generation Physiology: Chemical Probes to Visualize Bacterial Cell Structure and Physiology Open
Chemical probes have been instrumental in microbiology since its birth as a discipline in the 19th century when chemical dyes were used to visualize structural features of bacterial cells for the first time. In this review article we will …
View article: Structural Basis for the Inhibitor and Substrate Specificity of the Unique Fph Serine Hydrolases of <i>Staphylococcus aureus</i>
Structural Basis for the Inhibitor and Substrate Specificity of the Unique Fph Serine Hydrolases of <i>Staphylococcus aureus</i> Open
Staphylococcus aureus is a prevalent bacterial pathogen in both community and hospital settings, and its treatment is made particularly difficult by resilience within biofilms. Within this niche, serine hydrolase enzymes play a key role in…
View article: Structural basis for active-site probes targeting<i>Staphylococcus aureus</i>serine hydrolase virulence factors
Structural basis for active-site probes targeting<i>Staphylococcus aureus</i>serine hydrolase virulence factors Open
Summary Staphylococcus aureus is a major cause of infection in the community and in hospitals. Serine hydrolases play key roles in bacterial homeostasis, in particular biofilms. Activity-based profiling has previously identified a family o…
View article: What you see is what you get: activity-based probes in single-cell analysis of enzymatic activities
What you see is what you get: activity-based probes in single-cell analysis of enzymatic activities Open
Molecular imaging methods can provide spatio-temporal information about the distribution of biomolecules or biological processes, such as certain enzymatic activities, in single cells. Within a cell, it is possible to define the subcellula…
View article: An NAD+-dependent novel transcription factor controls stage conversion in Entamoeba
An NAD+-dependent novel transcription factor controls stage conversion in Entamoeba Open
Developmental switching between life-cycle stages is a common feature among parasitic pathogens to facilitate disease transmission and pathogenesis. The protozoan parasite Entamoeba switches between invasive trophozoites and dormant cysts,…
View article: Inhibition of NGLY1 Inactivates the Transcription Factor Nrf1 and Potentiates Proteasome Inhibitor Cytotoxicity
Inhibition of NGLY1 Inactivates the Transcription Factor Nrf1 and Potentiates Proteasome Inhibitor Cytotoxicity Open
Proteasome inhibitors are used to treat blood cancers such as multiple myeloma (MM) and mantle cell lymphoma. The efficacy of these drugs is frequently undermined by acquired resistance. One mechanism of proteasome inhibitor resistance may…
View article: Design of Selective Substrates and Activity-Based Probes for Hydrolase Important for Pathogenesis 1 (HIP1) from <i>Mycobacterium tuberculosis</i>
Design of Selective Substrates and Activity-Based Probes for Hydrolase Important for Pathogenesis 1 (HIP1) from <i>Mycobacterium tuberculosis</i> Open
Although serine proteases are important mediators of Mycobacterium tuberculosis (Mtb) virulence, there are currently no tools to selectively block or visualize members of this family of enzymes. Selective reporter substrates or activity-ba…