Christian Gnann
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View article: Decrypting lysine deacetylase inhibitor action and protein modifications by dose-resolved proteomics
Decrypting lysine deacetylase inhibitor action and protein modifications by dose-resolved proteomics Open
Lysine deacetylase inhibitors (KDACis) are approved drugs for cutaneous T cell lymphoma (CTCL), peripheral T cell lymphoma (PTCL), and multiple myeloma, but many aspects of their cellular mechanism of action (MoA) and substantial toxicity …
View article: Author Correction: Spatiotemporal dissection of the cell cycle with single-cell proteogenomics
Author Correction: Spatiotemporal dissection of the cell cycle with single-cell proteogenomics Open
View article: Deep Visual Proteomics defines single-cell identity and heterogeneity
Deep Visual Proteomics defines single-cell identity and heterogeneity Open
Despite the availabilty of imaging-based and mass-spectrometry-based methods for spatial proteomics, a key challenge remains connecting images with single-cell-resolution protein abundance measurements. Here, we introduce Deep Visual Prote…
View article: OpenCell: Endogenous tagging for the cartography of human cellular organization
OpenCell: Endogenous tagging for the cartography of human cellular organization Open
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interacti…
View article: OpenCell: proteome-scale endogenous tagging enables the cartography of human cellular organization
OpenCell: proteome-scale endogenous tagging enables the cartography of human cellular organization Open
Elucidating the wiring diagram of the human cell is a central goal of the post-genomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry and data science to systematically map the localization and interacti…
View article: AI-driven Deep Visual Proteomics defines cell identity and heterogeneity
AI-driven Deep Visual Proteomics defines cell identity and heterogeneity Open
The systems-wide analysis of biomolecules in time and space is key to our understanding of cellular function and heterogeneity in health and disease 1 . Remarkable technological progress in microscopy and multi-omics technologies enable in…
View article: Illuminating Non-genetic Cellular Heterogeneity with Imaging-Based Spatial Proteomics
Illuminating Non-genetic Cellular Heterogeneity with Imaging-Based Spatial Proteomics Open
Cellular heterogeneity is an important biological phenomenon observed across space and time in human tissues. Imaging-based spatial proteomic technologies can provide fruitful new readouts of phenotypic states for individual cells at subce…
View article: Mapping the nucleolar proteome reveals a spatiotemporal organization related to intrinsic protein disorder
Mapping the nucleolar proteome reveals a spatiotemporal organization related to intrinsic protein disorder Open
View article: A Sample Preparation Protocol for High Throughput Immunofluorescence of Suspension Cells on an Adherent Surface
A Sample Preparation Protocol for High Throughput Immunofluorescence of Suspension Cells on an Adherent Surface Open
Imaging is a powerful approach for studying protein expression and has the advantage over other methodologies in providing spatial information in situ at single cell level. Using immunofluorescence and confocal microscopy, detailed informa…
View article: Mapping the nucleolar proteome reveals a spatiotemporal organization related to intrinsic protein disorder
Mapping the nucleolar proteome reveals a spatiotemporal organization related to intrinsic protein disorder Open
The nucleolus is essential for ribosome biogenesis and is involved in many other cellular functions. We performed a systematic spatiotemporal dissection of the human nucleolar proteome using confocal microscopy. In total, 1,318 nucleolar p…
View article: A sample preparation protocol for high throughput immunofluorescence of suspension cells
A sample preparation protocol for high throughput immunofluorescence of suspension cells Open
Imaging is a powerful approach for studying protein expression and has the advantage over other methodologies in providing spatial information in situ at single cell level. Using immunofluorescence and confocal microscopy, detailed informa…
View article: Spatiotemporal dissection of the cell cycle with single-cell proteogenomics
Spatiotemporal dissection of the cell cycle with single-cell proteogenomics Open
Cellular division is a fundamental source of cell-to-cell variability, and studies of transcript and protein abundances have revealed several hundred genes that are regulated by the cell cycle 1–8 . However, none of these studies provide s…
View article: Spatiotemporal characterization of the human proteome.
Spatiotemporal characterization of the human proteome. Open
Characterizing the molecular components of the basic unit of life; the cell, is crucial for a complete understanding of human biology. The cell is divided into compartments to create a suitable environment for the resident proteins to fulf…