Christine L. Mummery
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View article: The ethical aspects of human organ-on-chip models: A mapping review
The ethical aspects of human organ-on-chip models: A mapping review Open
Organ-on-chips (OoCs) are controlled microfluidic platforms that replicate specific organ-level functionalities and pathological processes using cultured cells. OoCs promise to enhance drug discovery, reduce dependence on animal models, an…
View article: Tissue microenvironment dictates the state of human iPSC-derived endothelial cells of distinct developmental origin in 3D cardiac microtissues
Tissue microenvironment dictates the state of human iPSC-derived endothelial cells of distinct developmental origin in 3D cardiac microtissues Open
View article: Tapered Pillar Design for High‐Precision Force Readout in Miniaturized Engineered Heart Tissues From Human Pluripotent Stem Cells
Tapered Pillar Design for High‐Precision Force Readout in Miniaturized Engineered Heart Tissues From Human Pluripotent Stem Cells Open
Engineered heart tissues (EHTs) formed around flexible pillars are used to measure the contraction force of myocytes. When based on cardiac cells derived from human induced pluripotent stem cells (hiPSCs), EHTs capture human cardiac physio…
View article: Controlled Induction of ALK1 in <i>ACVRL1</i>-null hiPSC-derived Endothelial Cells Provides Insight into Organ-Restricted AVMs in HHT
Controlled Induction of ALK1 in <i>ACVRL1</i>-null hiPSC-derived Endothelial Cells Provides Insight into Organ-Restricted AVMs in HHT Open
Genetic alterations in activin receptor-like kinase 1 ( ACVRL1 , ALK1) are linked to hereditary hemorrhagic telangiectasia (HHT), a condition characterized by hemorrhages, arteriovenous malformations (AVMs) and endothelial cell (EC) dysfun…
View article: Generation of patient-derived and gene-corrected hiPSC lines from Hereditary Hemorrhagic Telangiectasia type 2 patients with ACVRL1 c.1042delG mutation
Generation of patient-derived and gene-corrected hiPSC lines from Hereditary Hemorrhagic Telangiectasia type 2 patients with ACVRL1 c.1042delG mutation Open
Hereditary Hemorrhagic Telangiectasia type 2 (HHT2) is a vascular disorder caused by mutations in ACVRL1. We generated human induced pluripotent stem cell (hiPSC) lines from two HHT2 patients with a heterozygous 1 bp deletion in exon 7 of …
View article: Promoting the adoption of best practices and standards to enhance quality and reproducibility of stem cell research
Promoting the adoption of best practices and standards to enhance quality and reproducibility of stem cell research Open
View article: Vascularization of organoid microenvironments: Perfusable networks for organoid growth and maturation
Vascularization of organoid microenvironments: Perfusable networks for organoid growth and maturation Open
View article: A simplified co-culture reveals altered cardiotoxic responses to doxorubicin in hPSC-derived cardiomyocytes in the presence of endothelial cells
A simplified co-culture reveals altered cardiotoxic responses to doxorubicin in hPSC-derived cardiomyocytes in the presence of endothelial cells Open
Cardiotoxicity is a significant challenge in cancer therapies, particularly with doxorubicin, a widely used anthracycline known for its broad anti-cancer spectrum but life-threatening cardiac side effects. There is a critical need for more…
View article: Ex vivo removal of pro-fibrotic collagen and rescue of metabolic function in human ovarian fibrosis
Ex vivo removal of pro-fibrotic collagen and rescue of metabolic function in human ovarian fibrosis Open
Tissue fibrosis, with the excessive accumulation of extracellular matrix, leads to organ dysfunction. The ovary shows signs of fibrosis from an early age, creating a permissive environment for ovarian cancer. A robust culture-platform to s…
View article: FORCETRACKER: A versatile tool for standardized assessment of tissue contractile properties in 3D Heart-on-Chip platforms
FORCETRACKER: A versatile tool for standardized assessment of tissue contractile properties in 3D Heart-on-Chip platforms Open
Engineered heart tissues (EHTs) have shown great potential in recapitulating tissue organization, functions, and cell-cell interactions of the human heart in vitro . Currently, multiple EHT platforms are used by both industry and academia …
View article: Revitalizing the heart: strategies and tools for cardiomyocyte regeneration post-myocardial infarction
Revitalizing the heart: strategies and tools for cardiomyocyte regeneration post-myocardial infarction Open
View article: Gene syntax defines supercoiling-mediated transcriptional feedback
Gene syntax defines supercoiling-mediated transcriptional feedback Open
Gene syntax—the order and arrangement of genes and their regulatory elements—shapes the dynamic coordination of both natural and synthetic gene circuits. Transcription at one locus profoundly impacts the transcription of nearby adjacent ge…
View article: Depletion of <i>TP53</i> in Human Pluripotent Stem Cells Triggers Malignant‐Like Behavior
Depletion of <i>TP53</i> in Human Pluripotent Stem Cells Triggers Malignant‐Like Behavior Open
Human pluripotent stem cells (hPSCs) tend to acquire genetic aberrations upon culture in vitro. Common aberrations are mutations in the tumor suppressor TP53 , suspected to confer a growth‐advantage to the mutant cells. However, their full…
View article: Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19
Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19 Open
COVID-19 plasma induces endothelial activation and thromboinflammation in a Vessel-on-Chip model, highlighting the causative role of systemic factors and offering a platform for studying severe COVID-19 pathophysiology and therapeutic inte…
View article: Global Literature Analysis of Tumor Organoid and Tumor-on-Chip Research
Global Literature Analysis of Tumor Organoid and Tumor-on-Chip Research Open
Background: Tumor organoid and tumor-on-chip (ToC) platforms replicate aspects of the anatomical and physiological states of tumors. They, therefore, serve as models for investigating tumor microenvironments, metastasis, and immune interac…
View article: Accessible desktop 3D printer engineering for scalable and reproducible iPSC-derived kidney organoid bioprinting
Accessible desktop 3D printer engineering for scalable and reproducible iPSC-derived kidney organoid bioprinting Open
View article: Industrialization of three-dimensional hiPSC-cardiac microtissues for high-throughput cardiac safety and drug discovery screening
Industrialization of three-dimensional hiPSC-cardiac microtissues for high-throughput cardiac safety and drug discovery screening Open
Summary Current cardiac cell models for drug screening often face a trade-off between cellular maturity and achieving high throughput. While three-dimensional human induced pluripotent stem cell-based heart models typically exhibit more ad…
View article: STRAIGHT-IN Dual: a platform for dual, single-copy integrations of DNA payloads and gene circuits into human induced pluripotent stem cell
STRAIGHT-IN Dual: a platform for dual, single-copy integrations of DNA payloads and gene circuits into human induced pluripotent stem cell Open
Targeting DNA payloads into human (h)iPSCs involves multiple time-consuming, inefficient steps that must be repeated for each construct. Here, we present STRAIGHT-IN Dual, which enables simultaneous, allele-specific, single-copy integratio…
View article: Generation of two induced pluripotent stem cell (iPSC) lines carrying the Brugada Syndrome-associated mutation SCN5A-R282H
Generation of two induced pluripotent stem cell (iPSC) lines carrying the Brugada Syndrome-associated mutation SCN5A-R282H Open
Brugada Syndrome (BrS) is a cardiac arrhythmia disorder which can lead to sudden cardiac death. It is commonly associated with loss-of-function mutations in the SCN5A gene, encoding the alpha subunit of the sodium voltage-gated channel. We…
View article: Spatial analysis of transcript and protein levels in skeletal muscle
Spatial analysis of transcript and protein levels in skeletal muscle Open
Skeletal muscle spatial analyses have revealed unexpected regionalized gene expression patterns challenging the understanding of muscle as a homogeneous tissue. Here, we present a protocol for the spatial analysis of transcript and protein…
View article: Cell cycle visualization tools to study cardiomyocyte proliferation in real-time
Cell cycle visualization tools to study cardiomyocyte proliferation in real-time Open
Cardiomyocytes in the adult human heart are quiescent and those lost following heart injury are not replaced by proliferating survivors. Considerable effort has been made to understand the mechanisms underlying cardiomyocyte cell cycle exi…
View article: Standardizing designed and emergent quantitative features in microphysiological systems
Standardizing designed and emergent quantitative features in microphysiological systems Open
View article: Engineered microvasculature using maskless photolithography and on-chip hydrogel patterning: a facile approach
Engineered microvasculature using maskless photolithography and on-chip hydrogel patterning: a facile approach Open
In vitro models of human microvasculature are increasingly used to understand blood vessel diseases and to support drug development. Most engineered models, however, are slow and labor-intensive to produce. Here, we used a single commercia…
View article: Generation and Characterization of hiPSC‐Derived Vascularized‐, Perfusable Cardiac Microtissues‐on‐Chip
Generation and Characterization of hiPSC‐Derived Vascularized‐, Perfusable Cardiac Microtissues‐on‐Chip Open
In the heart in vivo , vasculature forms a semi‐permeable endothelial barrier for selective nutrient and (immune) cell delivery to the myocardium and removal of waste products. Crosstalk between the vasculature and the heart cells regulate…
View article: Self-assembling 3D vessel-on-chip model with hiPSC-derived astrocytes
Self-assembling 3D vessel-on-chip model with hiPSC-derived astrocytes Open
Functionality of the blood-brain barrier (BBB) relies on the interaction between endothelial cells (ECs), pericytes, and astrocytes to regulate molecule transport within the central nervous system. Most experimental models for the BBB rely…
View article: Heart and vessels from stem cells: A short history of serendipity and good luck
Heart and vessels from stem cells: A short history of serendipity and good luck Open
Stem cell research is the product of cumulative, integrated effort between and within laboratories and disciplines. The many collaborative steps that lead to that special “Eureka moment”, when something that has been a puzzle perhaps for y…
View article: Three-dimensional cardiac models: a pre-clinical testing platform
Three-dimensional cardiac models: a pre-clinical testing platform Open
Major advancements in human pluripotent stem cell (hPSC) technology over recent years have yielded valuable tools for cardiovascular research. Multi-cell type 3-dimensional (3D) cardiac models in particular, are providing complementary app…
View article: The mechanistic role of connexin 43 in maturation of hiPSC cardiomyocytes
The mechanistic role of connexin 43 in maturation of hiPSC cardiomyocytes Open
Funding Acknowledgements Type of funding sources: Public grant(s) – EU funding. Main funding source(s): ECR (European research council) and ReNew (the Novo Nordisk Foundation Center for Stem Cell Medicine supported by Novo Nordisk Foundati…
View article: Maturing hiPSC-cardiomyocytes in cardiac microtissues for accurate preclinical in vitro modelling of long-QT syndrome type 1
Maturing hiPSC-cardiomyocytes in cardiac microtissues for accurate preclinical in vitro modelling of long-QT syndrome type 1 Open
Funding Acknowledgements Type of funding sources: Foundation. Main funding source(s): reNEW - Novo Nordisk Foundation Center for Stem Cell Medicine Background Long-QT syndrome type 1 (LQT1), a cardiac arrhythmia often leading to sudden car…
View article: Generation of isogenic allelic series of LMNA-mutated hiPSC lines using the novel and highly-efficient targeting platform, STRAIGHT-IN
Generation of isogenic allelic series of LMNA-mutated hiPSC lines using the novel and highly-efficient targeting platform, STRAIGHT-IN Open
Funding Acknowledgements Type of funding sources: Public grant(s) – National budget only. Main funding source(s): The Netherlands Organisation for Health Research and Development ZonMW Background Human induced pluripotent stem cells (hiPSC…