Christine von Toerne
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View article: Benchmarking porcine pancreatic ductal organoids for drug screening applications
Benchmarking porcine pancreatic ductal organoids for drug screening applications Open
Primary human pancreatic ductal organoids (HPDO) have emerged as a model to study pancreas biology and model disease like pancreatitis and pancreatic cancer. Yet, donor material availability, genetic variability and a lack of extensive ben…
View article: Multicenter evaluation of label-free quantification in human plasma on a high dynamic range benchmark set
Multicenter evaluation of label-free quantification in human plasma on a high dynamic range benchmark set Open
Human plasma is routinely collected during clinical care and constitutes a rich source of biomarkers for diagnostics and patient stratification. Liquid chromatography-mass spectrometry (LC-MS)-based proteomics is a key method for plasma bi…
View article: Correction to “Multicenter Longitudinal Quality Assessment of MS-Based Proteomics in Plasma and Serum”
Correction to “Multicenter Longitudinal Quality Assessment of MS-Based Proteomics in Plasma and Serum” Open
View article: Privacy-preserving multicenter differential protein abundance analysis with FedProt
Privacy-preserving multicenter differential protein abundance analysis with FedProt Open
Quantitative mass spectrometry has revolutionized proteomics by enabling simultaneous quantification of thousands of proteins. Pooling patient-derived data from multiple institutions enhances statistical power but raises serious privacy co…
View article: Sex differences in resting skeletal muscle and the acute and long-term response to endurance exercise in individuals with overweight and obesity
Sex differences in resting skeletal muscle and the acute and long-term response to endurance exercise in individuals with overweight and obesity Open
View article: Differential Expression of ARG1 and MRC2 in Retinal Müller Glial Cells During Autoimmune Uveitis
Differential Expression of ARG1 and MRC2 in Retinal Müller Glial Cells During Autoimmune Uveitis Open
Retinal Müller glial cells (RMG) play a crucial role in retinal neuroinflammation, including autoimmune uveitis. Increasing evidence supports their function as active modulators of immune responses and potential atypical antigen-presenting…
View article: Multicenter Longitudinal Quality Assessment of MS-Based Proteomics in Plasma and Serum
Multicenter Longitudinal Quality Assessment of MS-Based Proteomics in Plasma and Serum Open
Advancing MS-based proteomics toward clinical applications evolves around developing standardized start-to-finish and fit-for-purpose workflows for clinical specimens. Steps along the method design involve the determination and optimizatio…
View article: Multicenter Evaluation of Label-Free Quantification in Human Plasma: Benchmarking with a High Dynamic Range Multispecies Sample Set
Multicenter Evaluation of Label-Free Quantification in Human Plasma: Benchmarking with a High Dynamic Range Multispecies Sample Set Open
Human plasma is routinely collected during clinical care and constitutes a rich source of biomarkers for diagnostics and patient stratification. Liquid chromatography-mass spectrometry (LC-MS)-based proteomics is a key method for plasma bi…
View article: Sexual dimorphism in molecular profiles of resting human skeletal muscle and the response to acute exercise and endurance training
Sexual dimorphism in molecular profiles of resting human skeletal muscle and the response to acute exercise and endurance training Open
Biological sex has a strong impact on skeletal muscle metabolism and performance. By a comprehensive investigation of epigenetic, transcriptomic and proteomic differences between female and male skeletal muscle of untrained subjects we pro…
View article: Sex-Specific Differences of Human Skeletal Muscle: A Multi-Omics Exercise Study
Sex-Specific Differences of Human Skeletal Muscle: A Multi-Omics Exercise Study Open
View article: Cathepsin S Is More Abundant in Serum of Mycobacterium avium subsp. paratuberculosis-Infected Dairy Cows
Cathepsin S Is More Abundant in Serum of Mycobacterium avium subsp. paratuberculosis-Infected Dairy Cows Open
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of bovine paratuberculosis, a chronic granulomatous enteritis leading to economic losses and posing a risk to human health due to its zoonotic potential. The pathogen…
View article: Stem cell-derived vessels-on-chip for cardiovascular disease modeling
Stem cell-derived vessels-on-chip for cardiovascular disease modeling Open
The metabolic syndrome is accompanied by vascular complications. Human in vitro disease models are hence required to better understand vascular dysfunctions and guide clinical therapies. Here, we engineered an open microfluidic vessel-on-c…
View article: IGF1 promotes human myotube differentiation toward a mature metabolic and contractile phenotype
IGF1 promotes human myotube differentiation toward a mature metabolic and contractile phenotype Open
Human skeletal muscle models are highly valuable to study how exercise prevents type 2 diabetes without invasive biopsies. Current models did not fully recapitulate the function of skeletal muscle especially during exercise. By supplementi…
View article: The Chromosome Passenger Complex (CPC) Components and Its Associated Pathways Are Promising Candidates to Differentiate Between Normosensitive and Radiosensitive ATM-Mutated Cells
The Chromosome Passenger Complex (CPC) Components and Its Associated Pathways Are Promising Candidates to Differentiate Between Normosensitive and Radiosensitive ATM-Mutated Cells Open
Background: Sensitivity to ionizing radiation differs between individuals, but there is a limited understanding of the biological mechanisms that account for these variations. One example of such mechanisms are the mutations in the ATM (mu…
View article: Multicenter Collaborative Study to Optimize Mass Spectrometry Workflows of Clinical Specimens
Multicenter Collaborative Study to Optimize Mass Spectrometry Workflows of Clinical Specimens Open
The foundation for integrating mass spectrometry (MS)-based proteomics into systems medicine is the development of standardized start-to-finish and fit-for-purpose workflows for clinical specimens. An essential step in this pursuit is to h…
View article: Placebos meet proteomics
Placebos meet proteomics Open
The human proteome is defined as the overall set of proteins that can be expressed by genome, cells, tissues, or organisms at a given time. There are estimates that the proteome consists of over one hundred thousand proteins, not including…
View article: Engineering of human myotubes toward a mature metabolic and contractile phenotype
Engineering of human myotubes toward a mature metabolic and contractile phenotype Open
1. Abstract Skeletal muscle mediates the beneficial effects of exercise, thereby improving insulin sensitivity and reducing the risk for type 2 diabetes. Current human skeletal muscle models in vitro are incapable of fully recapitulating i…
View article: The miRNA Content of Bone Marrow-Derived Extracellular Vesicles Contributes to Protein Pathway Alterations Involved in Ionising Radiation-Induced Bystander Responses
The miRNA Content of Bone Marrow-Derived Extracellular Vesicles Contributes to Protein Pathway Alterations Involved in Ionising Radiation-Induced Bystander Responses Open
Extracellular vesicles (EVs), through their cargo, are important mediators of bystander responses in the irradiated bone marrow (BM). MiRNAs carried by EVs can potentially alter cellular pathways in EV-recipient cells by regulating their p…
View article: Micro-RNA and Proteomic Profiles of Plasma-Derived Exosomes from Irradiated Mice Reveal Molecular Changes Preventing Apoptosis in Neonatal Cerebellum
Micro-RNA and Proteomic Profiles of Plasma-Derived Exosomes from Irradiated Mice Reveal Molecular Changes Preventing Apoptosis in Neonatal Cerebellum Open
Cell communication via exosomes is capable of influencing cell fate in stress situations such as exposure to ionizing radiation. In vitro and in vivo studies have shown that exosomes might play a role in out-of-target radiation effects by …
View article: Adipose microtissue-on-chip: a 3D cell culture platform for differentiation, stimulation, and proteomic analysis of human adipocytes
Adipose microtissue-on-chip: a 3D cell culture platform for differentiation, stimulation, and proteomic analysis of human adipocytes Open
In this study, we developed a microfluidic large-scale integration chip platform to automate longitudinal 3D cell culture studies. Proteome analysis of on chip cultured adipocytes under changing glucose conditions revealed metabolic stress…
View article: A Human 3D Cardiomyocyte Risk Model to Study the Cardiotoxic Influence of X-rays and Other Noxae in Adults
A Human 3D Cardiomyocyte Risk Model to Study the Cardiotoxic Influence of X-rays and Other Noxae in Adults Open
The heart tissue is a potential target of various noxae contributing to the onset of cardiovascular diseases. However, underlying pathophysiological mechanisms are largely unknown. Human stem cell-derived models are promising, but a major …
View article: Targeting Cancer Metabolism Breaks Radioresistance by Impairing the Stress Response
Targeting Cancer Metabolism Breaks Radioresistance by Impairing the Stress Response Open
The heightened energetic demand increases lactate dehydrogenase (LDH) activity, the corresponding oncometabolite lactate, expression of heat shock proteins (HSPs) and thereby promotes therapy resistance in many malignant tumor cell types. …
View article: Single-cell-resolved differentiation of human induced pluripotent stem cells into pancreatic duct-like organoids on a microwell chip
Single-cell-resolved differentiation of human induced pluripotent stem cells into pancreatic duct-like organoids on a microwell chip Open
View article: Data-Independent Acquisition Proteomics Reveals Long-Term Biomarkers in the Serum of C57BL/6J Mice Following Local High-Dose Heart Irradiation
Data-Independent Acquisition Proteomics Reveals Long-Term Biomarkers in the Serum of C57BL/6J Mice Following Local High-Dose Heart Irradiation Open
Background and Purpose: Cardiotoxicity is a well-known adverse effect of radiation therapy. Measurable abnormalities in the heart function indicate advanced and often irreversible heart damage. Therefore, early detection of cardiac toxicit…
View article: Out-of-Field Hippocampus from Partial-Body Irradiated Mice Displays Changes in Multi-Omics Profile and Defects in Neurogenesis
Out-of-Field Hippocampus from Partial-Body Irradiated Mice Displays Changes in Multi-Omics Profile and Defects in Neurogenesis Open
The brain undergoes ionizing radiation exposure in many clinical situations, particularly during radiotherapy for brain tumors. The critical role of the hippocampus in the pathogenesis of radiation-induced neurocognitive dysfunction is wel…
View article: Lipocalin 13 enhances insulin secretion but is dispensable for systemic metabolic control
Lipocalin 13 enhances insulin secretion but is dispensable for systemic metabolic control Open
Members of the lipocalin protein family serve as biomarkers for kidney disease and acute phase inflammatory reactions, and are under preclinical development for the diagnosis and therapy of allergies. However, none of the lipocalin family …
View article: Radiation Response of Human Cardiac Endothelial Cells Reveals a Central Role of the cGAS-STING Pathway in the Development of Inflammation
Radiation Response of Human Cardiac Endothelial Cells Reveals a Central Role of the cGAS-STING Pathway in the Development of Inflammation Open
Radiation-induced inflammation leading to the permeability of the endothelial barrier may increase the risk of cardiovascular disease. The aim of this study was to investigate potential mechanisms in vitro at the level of the proteome in h…
View article: Molecular classification of the placebo effect in nausea
Molecular classification of the placebo effect in nausea Open
In this proof-of-concept study, we tested whether placebo effects can be monitored and predicted by plasma proteins. In a randomized controlled design, 90 participants were exposed to a nauseating stimulus on two separate days and were ran…
View article: Deciphering the Plasma Proteome of Type 2 Diabetes
Deciphering the Plasma Proteome of Type 2 Diabetes Open
With an estimated prevalence of 463 million affected, type 2 diabetes represents a major challenge to health care systems worldwide. Analyzing the plasma proteomes of individuals with type 2 diabetes may illuminate hitherto unknown functio…
View article: Deciphering the Plasma Proteome of Type 2 Diabetes
Deciphering the Plasma Proteome of Type 2 Diabetes Open
With an estimated prevalence of 463 million affected, type 2 diabetes represents a major challenge to health care systems worldwide. Analyzing the plasma proteomes of individuals with type 2 diabetes may illuminate hitherto unknown functio…