Christopher A. Penfold
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View article: Digital Reprogramming Decodes Epigenetic Barriers of Cell Fate Changes
Digital Reprogramming Decodes Epigenetic Barriers of Cell Fate Changes Open
Fates of differentiated cells in our body can be induced to change by nuclear reprogramming. In this way, cells valuable for therapeutic purposes and disease modeling can be produced. However, the efficiency of this process is low, partly …
View article: Primitive to visceral endoderm maturation is essential for mouse epiblast survival beyond implantation
Primitive to visceral endoderm maturation is essential for mouse epiblast survival beyond implantation Open
The implantation of the mouse blastocyst initiates a complex sequence of tissue remodeling and cell differentiation events required for morphogenesis, during which the extraembryonic primitive endoderm transitions into the visceral endoder…
View article: Marmoset and human trophoblast stem cells differ in signaling requirements and recapitulate divergent modes of trophoblast invasion
Marmoset and human trophoblast stem cells differ in signaling requirements and recapitulate divergent modes of trophoblast invasion Open
Early human trophoblast development has remained elusive due to the inaccessibility of the early conceptus. Non-human primate models recapitulate many features of human development and allow access to early postimplantation stages. Here, w…
View article: Featured Cover
Featured Cover Open
Cover legend: The cover image is based on the Article Age‐related and species‐specific methylation changes in the protein‐coding marmoset sperm epigenome by Marcus Dittrich et al., https://doi.org/10.1111/acel.14200.[Image: see text]
View article: Age‐related and species‐specific methylation changes in the protein‐coding marmoset sperm epigenome
Age‐related and species‐specific methylation changes in the protein‐coding marmoset sperm epigenome Open
The sperm epigenome is thought to affect the developmental programming of the resulting embryo, influencing health and disease in later life. Age‐related methylation changes in the sperm of old fathers may mediate the increased risks for r…
View article: mRNA-based generation of marmoset PGCLCs capable of differentiation into gonocyte-like cells
mRNA-based generation of marmoset PGCLCs capable of differentiation into gonocyte-like cells Open
Primate germ cell development remains largely unexplored due to limitations in sample collection and the long duration of development. In mice, primordial germ cell-like cells (PGCLCs) derived from pluripotent stem cells (PSCs) can develop…
View article: Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells
Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells Open
In mammals, pluripotent cells transit through a continuum of distinct molecular and functional states en route to initiating lineage specification. Capturing pluripotent stem cells (PSCs) mirroring in vivo pluripotent states provide…
View article: Origin and segregation of the human germline
Origin and segregation of the human germline Open
Human germline–soma segregation occurs during weeks 2–3 in gastrulating embryos. Although direct studies are hindered, here, we investigate the dynamics of human primordial germ cell (PGCs) specification using in vitro models with temporal…
View article: Epigenetic resetting in the human germ line entails histone modification remodeling
Epigenetic resetting in the human germ line entails histone modification remodeling Open
Epigenetic resetting in the mammalian germ line entails acute DNA demethylation, which lays the foundation for gametogenesis, totipotency, and embryonic development. We characterize the epigenome of hypomethylated human primordial germ cel…
View article: Spatial embryo profiling (STEP) of primate implantation stages
Spatial embryo profiling (STEP) of primate implantation stages Open
Spatial embryo profiling (STEP) combines laser capture microdissection (LCM)-assisted sample collection for Smart-Seq2 profiling with virtual reconstruction of stereological immunofluorescence images of implanted primate embryos. Frozen, u…
View article: mRNA-based generation of marmoset PGCLCs capable of differentiation into gonocyte-like cells
mRNA-based generation of marmoset PGCLCs capable of differentiation into gonocyte-like cells Open
Summary Primate germ cell development remains largely unexplored due to limitations in sample collection and the long duration of development. In mice, primordial germ cell-like cells (PGCLCs) derived from pluripotent stem cells (PSCs) can…
View article: Microgel culture and spatial identity mapping elucidate the signalling requirements for primate epiblast and amnion formation
Microgel culture and spatial identity mapping elucidate the signalling requirements for primate epiblast and amnion formation Open
The early specification and rapid growth of extraembryonic membranes are distinctive hallmarks of primate embryogenesis. These complex tasks are resolved through an intricate combination of signals controlling the induction of extraembryon…
View article: Origin and segregation of the human germline
Origin and segregation of the human germline Open
Human germline-soma segregation occurs during weeks 2-3 in gastrulating embryos. While direct studies are hindered, here we investigate the dynamics of human primordial germ cell (PGCs) specification using in vitro models with temporally r…
View article: An integrated atlas of human placental development delineates essential regulators of trophoblast stem cells
An integrated atlas of human placental development delineates essential regulators of trophoblast stem cells Open
The trophoblast lineage safeguards fetal development by mediating embryo implantation, immune tolerance, nutritional supply and gas exchange. Human trophoblast stem cells (hTSCs) provide a platform to study lineage specification of placent…
View article: A hexa-species transcriptome atlas of mammalian embryogenesis delineates metabolic regulation across three different implantation modes
A hexa-species transcriptome atlas of mammalian embryogenesis delineates metabolic regulation across three different implantation modes Open
View article: Spatial profiling of early primate gastrulation in utero
Spatial profiling of early primate gastrulation in utero Open
View article: Nuclear transfer system for the direct induction of embryonic transcripts from intra- and cross-species nuclei using mouse 4-cell embryos
Nuclear transfer system for the direct induction of embryonic transcripts from intra- and cross-species nuclei using mouse 4-cell embryos Open
View article: Sequential enhancer state remodelling defines human germline competence and specification
Sequential enhancer state remodelling defines human germline competence and specification Open
View article: Mapping the biogenesis of forward programmed megakaryocytes from induced pluripotent stem cells
Mapping the biogenesis of forward programmed megakaryocytes from induced pluripotent stem cells Open
Platelet deficiency, known as thrombocytopenia, can cause hemorrhage and is treated with platelet transfusions. We developed a system for the production of platelet precursor cells, megakaryocytes, from pluripotent stem cells. These cultur…
View article: Cell division- and DNA replication-free reprogramming of somatic nuclei for embryonic transcription
Cell division- and DNA replication-free reprogramming of somatic nuclei for embryonic transcription Open
Nuclear transfer systems represent the efficient means to reprogram a cell and in theory provide a basis for investigating the development of endangered species. However, conventional nuclear transfer using oocytes of laboratory animals do…
View article: Tracing the emergence of primordial germ cells from bilaminar disc rabbit embryos and pluripotent stem cells
Tracing the emergence of primordial germ cells from bilaminar disc rabbit embryos and pluripotent stem cells Open
Rabbit embryos develop as bilaminar discs at gastrulation as in humans and most other mammals, whereas rodents develop as egg cylinders. Primordial germ cells (PGCs) appear to originate during gastrulation according to many systematic stud…
View article: Time‐series transcriptomics reveals a <i>BBX32</i>‐directed control of acclimation to high light in mature <i>Arabidopsis</i> leaves
Time‐series transcriptomics reveals a <i>BBX32</i>‐directed control of acclimation to high light in mature <i>Arabidopsis</i> leaves Open
SUMMARY The photosynthetic capacity of mature leaves increases after several days’ exposure to constant or intermittent episodes of high light (HL) and is manifested primarily as changes in chloroplast physiology. How this chloroplast‐leve…
View article: Building a stem cell-based primate uterus
Building a stem cell-based primate uterus Open
View article: Mapping the biogenesis of forward programmed megakaryocytes from induced pluripotent stem cells
Mapping the biogenesis of forward programmed megakaryocytes from induced pluripotent stem cells Open
Summary Platelet deficiency, known as thrombocytopenia, can cause haemorrhage and is treated with platelet transfusions. We developed a system for the production of platelet precursor cells, megakaryocytes, from pluripotent stem cells. The…
View article: Time series transcriptomics reveals a<i>BBX32</i>-directed control of dynamic acclimation to high light in mature<i>Arabidopsis</i>leaves
Time series transcriptomics reveals a<i>BBX32</i>-directed control of dynamic acclimation to high light in mature<i>Arabidopsis</i>leaves Open
SUMMARY The photosynthetic capacity of mature leaves increases after several days’ exposure to constant or intermittent episodes of high light (HL) and is manifested primarily as changes in chloroplast physiology. This is termed dynamic ac…
View article: Bayesian parameter estimation in non-stationary semiflexible polymers from ensembles of trajectories
Bayesian parameter estimation in non-stationary semiflexible polymers from ensembles of trajectories Open
During the cell-cycle and meiosis, during development, or in response to stress, chromosomes undertake dramatic programs of reorganisation, which can result in major changes to genomic architecture, as well as local changes to chromatin st…
View article: Branch-recombinant Gaussian processes for analysis of perturbations in biological time series
Branch-recombinant Gaussian processes for analysis of perturbations in biological time series Open
Motivation A common class of behaviour encountered in the biological sciences involves branching and recombination. During branching, a statistical process bifurcates resulting in two or more potentially correlated processes that may under…
View article: Comparative transcriptome analysis in Arabidopsis ein2/ore3 and ahk3/ore12 mutants during dark-induced leaf senescence
Comparative transcriptome analysis in Arabidopsis ein2/ore3 and ahk3/ore12 mutants during dark-induced leaf senescence Open
Leaf senescence involves degenerative but active biological processes that require balanced regulation of pro- and anti-senescing activities. Ethylene and cytokinin are major antagonistic regulatory hormones that control the timing and pro…
View article: Branch-recombinant Gaussian processes for analysis of perturbations in biological time series
Branch-recombinant Gaussian processes for analysis of perturbations in biological time series Open
Motivation A common class of behaviour encountered in the biological sciences involves branching and recombination. During branching, a statistical process bifurcates resulting in two or more potentially correlated processes that may under…
View article: Stella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transition
Stella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transition Open
The maternal-to-zygotic transition (MZT) marks the period when the embryonic genome is activated and acquires control of development. Maternally inherited factors play a key role in this critical developmental process, which occurs at the …