Anthony C. Ashton
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View article: Properties of Synaptic Vesicle Pools in Mature Central Nerve Terminals
Properties of Synaptic Vesicle Pools in Mature Central Nerve Terminals Open
Readily releasable and reserve pools of synaptic vesicles play different roles in neurotransmission, and it is important to understand their recycling and interchange in mature central synapses. Using adult rat cerebrocortical synaptosomes…
View article: Mutant α-Latrotoxin (LTXN4C) Does Not Form Pores and Causes Secretion by Receptor Stimulation
Mutant α-Latrotoxin (LTXN4C) Does Not Form Pores and Causes Secretion by Receptor Stimulation Open
Alpha-latrotoxin (LTX) causes massive release of neurotransmitters via a complex mechanism involving (i) activation of receptor(s) and (ii) toxin insertion into the plasma membrane with (iii) subsequent pore formation. Using cryo-electron …
View article: α-Latrotoxin, Acting via Two Ca2+-dependent Pathways, Triggers Exocytosis of Two Pools of Synaptic Vesicles
α-Latrotoxin, Acting via Two Ca2+-dependent Pathways, Triggers Exocytosis of Two Pools of Synaptic Vesicles Open
alpha-Latrotoxin stimulates three types of [(3)H]gamma-aminobutyric acid and [(14)C]glutamate release from synaptosomes. The Ca(2+)-independent component (i) is insensitive to SNAP-25 cleavage or depletion of vesicle contents by bafilomyci…
View article: A Late Phase of Exocytosis from Synaptosomes Induced by Elevated [Ca<sup>2+</sup>]<sub>i</sub> Is Not Blocked by <i>Clostridial</i> Neurotoxins
A Late Phase of Exocytosis from Synaptosomes Induced by Elevated [Ca<sup>2+</sup>]<sub>i</sub> Is Not Blocked by <i>Clostridial</i> Neurotoxins Open
Treatment of rat cerebrocortical synaptosomes with botulinum toxin types E and C1 or tetanus toxin removed the majority of intact SNAP‐25, syntaxin 1A/1B, and synaptobrevin and diminished Ca 2+ ‐dependent K + depolarization‐induced noradre…
View article: Norepinephrine exocytosis stimulated by α–latrotoxin requires both external and stored Ca <sup>2+</sup> and is mediated by latrophilin, G proteins and phospholipase C
Norepinephrine exocytosis stimulated by α–latrotoxin requires both external and stored Ca <sup>2+</sup> and is mediated by latrophilin, G proteins and phospholipase C Open
α–latrotoxin (LTX) stimulates massive release of neurotransmitters by binding to a heptahelical transmembrane protein, latrophilin. Our experiments demonstrate that latrophilin is a G–protein–coupled receptor that specifically associates w…
View article: Tetanus Toxin Inhibits Neuroexocytosis Even When Its Zn2+-dependent Protease Activity Is Removed
Tetanus Toxin Inhibits Neuroexocytosis Even When Its Zn2+-dependent Protease Activity Is Removed Open
Tetanus toxin (TeTX) is a dichain protein that blocks neuroexocytosis, an action attributed previously to Zn(2+)-dependent proteolysis of synaptobrevin (Sbr) by its light chain (LC). Herein, its cleavage of Sbr in rat cerebrocortical synap…
View article: Botulinum toxin A blocks glutamate exocytosis from guinea-pig cerebral cortical synaptosomes
Botulinum toxin A blocks glutamate exocytosis from guinea-pig cerebral cortical synaptosomes Open
The exocytotic release of L-glutamate from guinea-pig cerebral cortical synaptosomes can be extensively inhibited by preincubation with botulinum neurotoxin type A at 37 degrees C for 1-2 h. The toxin has no effect on synaptosomal respirat…