Christopher Cozens
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View article: Catalysts from synthetic genetic polymers
Catalysts from synthetic genetic polymers Open
The emergence of catalysis in early genetic polymers like RNA is considered a key transition in the origin of life1, predating the appearance of protein enzymes. DNA also demonstrates the capacity to fold into three-dimensional structures …
View article: Machine learning‐driven protein engineering: a case study in computational drug discovery
Machine learning‐driven protein engineering: a case study in computational drug discovery Open
Research and development in drug discovery will need to find significant efficiency gains if the industry is to continue generating novel drugs. There is great expectation for machine learning (ML) to provide this boost in R&D productivity…
View article: Kinetic analysis of <i>N</i>-alkylaryl carboxamide hexitol nucleotides as substrates for evolved polymerases
Kinetic analysis of <i>N</i>-alkylaryl carboxamide hexitol nucleotides as substrates for evolved polymerases Open
Six 1',5'-anhydrohexitol uridine triphosphates were synthesized with aromatic substitutions appended via a carboxamide linker to the 5-position of their bases. An improved method for obtaining such 5-substituted hexitol nucleosides and nuc…
View article: Phosphonomethyl Oligonucleotides as Backbone-Modified Artificial Genetic Polymers
Phosphonomethyl Oligonucleotides as Backbone-Modified Artificial Genetic Polymers Open
Although several synthetic or xenobiotic nucleic acids (XNAs) have been shown to be viable genetic materials in vitro, major hurdles remain for their in vivo applications, particularly orthogonality. The availability of XNAs that do not in…
View article: Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis
Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis Open
Engineering proteins for designer functions and biotechnological applications almost invariably requires (or at least benefits from) multiple mutations to non-contiguous residues. Several methods for multiple site-directed mutagenesis exis…
View article: Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis
Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis Open
Engineering proteins for designer functions and biotechnological applications almost invariably requires (or at least benefits from) multiple mutations to non-contiguous residues. Several methods for multiple site-directed mutagenesis exis…
View article: Enzymatic Synthesis of Nucleic Acids with Defined Regioisomeric 2′‐5′ Linkages
Enzymatic Synthesis of Nucleic Acids with Defined Regioisomeric 2′‐5′ Linkages Open
Information‐bearing nucleic acids display universal 3′‐5′ linkages, but regioisomeric 2′‐5′ linkages occur sporadically in non‐enzymatic RNA synthesis and may have aided prebiotic RNA replication. Herein we report on the enzymatic synthesi…
View article: Selection of 2′-deoxy-2′-fluoroarabinonucleotide (FANA) aptamers that bind HIV-1 reverse transcriptase with picomolar affinity
Selection of 2′-deoxy-2′-fluoroarabinonucleotide (FANA) aptamers that bind HIV-1 reverse transcriptase with picomolar affinity Open
Using a Systematic Evolution of Ligands by Exponential Enrichment (SELEX) protocol capable of selecting xeno-nucleic acid (XNA) aptamers, a 2'-deoxy-2'-fluoroarabinonucleotide (FANA) aptamer (referred to as FA1) to HIV-1 reverse transcript…