Christopher E. Lawson
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View article: Selective and Solvent-Free Extraction of Medium-Chain Fatty Acids with Polydimethylsiloxane Membranes
Selective and Solvent-Free Extraction of Medium-Chain Fatty Acids with Polydimethylsiloxane Membranes Open
Microbial production of medium-chain fatty acids (MCFAs) through anaerobic digestion of organic wastes has great potential as a method for sustainable chemical production owing to the high economic value of MCFAs, which are used in cosmeti…
View article: Engineering anaerobic fungal-bacterial consortia for medium-chain fatty acid production from lignocellulosic biomass
Engineering anaerobic fungal-bacterial consortia for medium-chain fatty acid production from lignocellulosic biomass Open
Anaerobic fermentation of lignocellulosic biomass offers a cost-effective route for sustainable biomanufacturing, yet constructing synthetic consortia for targeted bioproduction remains challenging. Inspired by herbivore rumen microbiome, …
View article: Carbon-efficient waste upcycling: combining syngas fermentation and chain elongation with synthetic consortia
Carbon-efficient waste upcycling: combining syngas fermentation and chain elongation with synthetic consortia Open
Syngas fermentation and chain elongation are key anaerobic biotechnologies for waste carbon upcycling. Their integration as a mixotrophic process enables simultaneous conversion of gaseous and wet waste substrates into medium-chain carboxy…
View article: Enabling Plasmid-based Expression in <i>Clostridium kluyveri</i> using a Biparental Methylation-Conjugation System
Enabling Plasmid-based Expression in <i>Clostridium kluyveri</i> using a Biparental Methylation-Conjugation System Open
Clostridium kluyveri is a promising biocatalyst for producing medium-chain fatty acids (MCFAs) from waste-derived carbon via chain elongation. MCFAs are platform chemicals with diverse applications across agriculture, food, cosmetics, and …
View article: Distinct Acetate Utilization Strategies Differentiate Butyrate and Octanoate Producing Chain-Elongating Bacteria
Distinct Acetate Utilization Strategies Differentiate Butyrate and Octanoate Producing Chain-Elongating Bacteria Open
Chain elongating bacteria (CEB) are a unique guild of anaerobes that convert lactate, ethanol, and acetate derived from organic waste into valuable short- and medium-chain fatty acids (MCFAs). However, the metabolic rules that determine pr…
View article: Machine learning-led semi-automated medium optimization reveals salt as key for flaviolin production in Pseudomonas putida
Machine learning-led semi-automated medium optimization reveals salt as key for flaviolin production in Pseudomonas putida Open
Although synthetic biology can produce valuable chemicals in a renewable manner, its progress is still hindered by a lack of predictive capabilities. Media optimization is a critical, and often overlooked, process which is essential to obt…
View article: Distinct lactate utilization strategies drive niche differentiation between two co-existing <i>Megasphaera</i> species in the rumen microbiome
Distinct lactate utilization strategies drive niche differentiation between two co-existing <i>Megasphaera</i> species in the rumen microbiome Open
Lactate utilization mitigates rumen acidosis and is associated with decreased methane production in the rumen. While several lactate utilization pathways exist across different microbial species in the rumen, how they are metabolically dif…
View article: Machine learning-led semi-automated medium optimization reveals salt as key for flaviolin production in Pseudomonas putida
Machine learning-led semi-automated medium optimization reveals salt as key for flaviolin production in Pseudomonas putida Open
Although synthetic biology can produce valuable chemicals in a renewable manner, its progress is still hindered by a lack of predictive capabilities. Media optimization is a critical, and often overlooked, process which is essential to obt…
View article: Evaluating the feasibility of medium chain oleochemical synthesis using microbial chain elongation
Evaluating the feasibility of medium chain oleochemical synthesis using microbial chain elongation Open
Chain elongating bacteria are a unique guild of strictly anaerobic bacteria that have garnered interest for sustainable chemical manufacturing from carbon-rich wet and gaseous waste streams. They produce C 6 -C 8 medium-chain fatty acids w…
View article: Impact of cathodic pH and bioaugmentation on acetate and CH<sub>4</sub> production in a microbial electrosynthesis cell
Impact of cathodic pH and bioaugmentation on acetate and CH<sub>4</sub> production in a microbial electrosynthesis cell Open
This study compares carbon dioxide conversion in carbonate-fed microbial electrosynthesis (MES) cells operated at low (5.3), neutral (7) and high (8) pH levels and inoculated either with wild-type or bioaugmented mixed microbial population…
View article: Evaluating the feasibility of medium-chain oleochemical synthesis using microbial chain elongation
Evaluating the feasibility of medium-chain oleochemical synthesis using microbial chain elongation Open
Chain elongating bacteria are a unique guild of strictly anaerobic bacteria that have garnered interest for sustainable chemical manufacturing from carbon-rich wet and gaseous waste streams. They produce C6–C8 medium-chain fatty acids, whi…
View article: Retooling Microbiome Engineering for a Sustainable Future
Retooling Microbiome Engineering for a Sustainable Future Open
Microbial communities (microbiomes) have been harnessed in biotechnology applications such as wastewater treatment and bioremediation for over a century. Traditionally, engineering approaches have focused on shaping the environment to stee…
View article: Investigating the Chemolithoautotrophic and Formate Metabolism of Nitrospira moscoviensis by Constraint-Based Metabolic Modeling and <sup>13</sup> C-Tracer Analysis
Investigating the Chemolithoautotrophic and Formate Metabolism of Nitrospira moscoviensis by Constraint-Based Metabolic Modeling and <sup>13</sup> C-Tracer Analysis Open
Nitrospira spp. are globally abundant nitrifying bacteria in soil and aquatic ecosystems and in wastewater treatment plants, where they control the oxidation of nitrite to nitrate. Despite their critical contribution to nitrogen cycling ac…
View article: A general approach to explore prokaryotic protein glycosylation reveals the unique surface layer modulation of an anammox bacterium
A general approach to explore prokaryotic protein glycosylation reveals the unique surface layer modulation of an anammox bacterium Open
The enormous chemical diversity and strain variability of prokaryotic protein glycosylation makes their large-scale exploration exceptionally challenging. Therefore, despite the universal relevance of protein glycosylation across all domai…
View article: Investigating the chemolithoautotrophic and formate metabolism of<i>Nitrospira moscoviensis</i>by constraint-based metabolic modeling and<sup>13</sup>C-tracer analysis
Investigating the chemolithoautotrophic and formate metabolism of<i>Nitrospira moscoviensis</i>by constraint-based metabolic modeling and<sup>13</sup>C-tracer analysis Open
Nitrite-oxidizing bacteria belonging to the genus Nitrospira mediate a key step in nitrification and play important roles in the biogeochemical nitrogen cycle and wastewater treatment. While these organisms have recently been shown to exhi…
View article: Experimentally Validated Reconstruction and Analysis of a Genome-Scale Metabolic Model of an Anaerobic Neocallimastigomycota Fungus
Experimentally Validated Reconstruction and Analysis of a Genome-Scale Metabolic Model of an Anaerobic Neocallimastigomycota Fungus Open
Recent genomic analyses have revealed that anaerobic gut fungi possess both the largest number and highest diversity of lignocellulolytic enzymes of all sequenced fungi, explaining their ability to decompose lignocellulosic substrates, e.g…
View article: Plant-wide systems microbiology for the wastewater industry
Plant-wide systems microbiology for the wastewater industry Open
Infiltrating systems microbiology concepts in the water industry requires the development of a language of love and shared endpoints in analytical bioscience and process engineering at the (waste)water line.
View article: A general approach to explore prokaryotic protein glycosylation reveals the unique surface layer modulation of an anammox bacterium
A general approach to explore prokaryotic protein glycosylation reveals the unique surface layer modulation of an anammox bacterium Open
The enormous chemical diversity and strain variability of prokaryotic protein glycosylation makes a large-scale exploration exceptionally challenging. Therefore, despite the universal relevance of protein glycosylation across all domains o…
View article: Machine learning for metabolic engineering: A review
Machine learning for metabolic engineering: A review Open
Machine learning provides researchers a unique opportunity to make metabolic engineering more predictable. In this review, we offer an introduction to this discipline in terms that are relatable to metabolic engineers, as well as providing…
View article: Autotrophic and mixotrophic metabolism of an anammox bacterium revealed by in vivo 13C and 2H metabolic network mapping
Autotrophic and mixotrophic metabolism of an anammox bacterium revealed by in vivo 13C and 2H metabolic network mapping Open
Anaerobic ammonium-oxidizing (anammox) bacteria mediate a key step in the biogeochemical nitrogen cycle and have been applied worldwide for the energy-efficient removal of nitrogen from wastewater. However, outside their core energy metabo…
View article: The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans
The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans Open
Six CO 2 fixation pathways are known to operate in photoautotrophic and chemoautotrophic microorganisms. Here, we describe chemolithoautotrophic growth of the sulphate-reducing bacterium Desulfovibrio desulfuricans (strain G11) with hydrog…
View article: Autotrophic and mixotrophic metabolism of an anammox bacterium revealed by <i>in vivo</i><sup>13</sup>C and <sup>2</sup>H metabolic network mapping
Autotrophic and mixotrophic metabolism of an anammox bacterium revealed by <i>in vivo</i><sup>13</sup>C and <sup>2</sup>H metabolic network mapping Open
Anaerobic ammonium-oxidizing (anammox) bacteria mediate a key step in the biogeochemical nitrogen cycle and have been applied worldwide for the energy-efficient removal of nitrogen from wastewater. However, outside their core energy metabo…
View article: Cultivation and Transcriptional Analysis of a Canonical Nitrospira Under Stable Growth Conditions
Cultivation and Transcriptional Analysis of a Canonical Nitrospira Under Stable Growth Conditions Open
Nitrite-oxidizing bacteria (NOB) are vital players in the global nitrogen cycle that convert nitrite to nitrate during the second step of nitrification. Within this functional guild, members of the genus Nitrospira are most widespread, phy…
View article: Metatranscriptomic and Thermodynamic Insights into Medium-Chain Fatty Acid Production Using an Anaerobic Microbiome
Metatranscriptomic and Thermodynamic Insights into Medium-Chain Fatty Acid Production Using an Anaerobic Microbiome Open
Mixed communities of microbes play important roles in health, the environment, agriculture, and biotechnology. While tapping the combined activities of organisms within microbiomes may allow the utilization of a wider range of substrates i…
View article: Metatranscriptomic and thermodynamic insights into medium-chain fatty acid production using an anaerobic microbiome
Metatranscriptomic and thermodynamic insights into medium-chain fatty acid production using an anaerobic microbiome Open
Biomanufacturing from renewable feedstocks represents a sustainable strategy to offset fossil fuel-based chemical production. One potential biomanufacturing strategy is production of medium chain fatty acids (MCFA) from organic feedstocks …