Christopher M. Humphreys
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View article: Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations
Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations Open
Base editors are recent multiplex gene editing tools derived from the Cas9 nuclease of Streptomyces pyogenes . They can target and modify a single nucleotide in the genome without inducing double-strand breaks (DSB) of the DNA helix. As su…
View article: Pathogenicity and virulence of <i>Clostridium botulinum</i>
Pathogenicity and virulence of <i>Clostridium botulinum</i> Open
Clostridium botulinum, a polyphyletic Gram-positive taxon of bacteria, is classified purely by their ability to produce botulinum neurotoxin (BoNT). BoNT is the primary virulence factor and the causative agent of botulism. A potenti…
View article: Enabling Ethanologenesis in Moorella thermoacetica through Construction of a Replicating Shuttle Vector
Enabling Ethanologenesis in Moorella thermoacetica through Construction of a Replicating Shuttle Vector Open
Replicating plasmid shuttle vectors are key tools for efficient genetic and metabolic engineering applications, allowing the development of sustainable bioprocesses using non-model organisms with unique metabolic capabilities. To date, ver…
View article: Design, Analysis, and Implementation of a Novel Biochemical Pathway for Ethylene Glycol Production in <i>Clostridium autoethanogenum</i>
Design, Analysis, and Implementation of a Novel Biochemical Pathway for Ethylene Glycol Production in <i>Clostridium autoethanogenum</i> Open
The platform chemical ethylene glycol (EG) is used to manufacture various commodity chemicals of industrial importance, but largely remains synthesized from fossil fuels. Although several novel metabolic pathways have been reported for its…
View article: Required Gene Set for Autotrophic Growth of <i>Clostridium autoethanogenum</i>
Required Gene Set for Autotrophic Growth of <i>Clostridium autoethanogenum</i> Open
Although microbial genome sequences are relatively easily determined, assigning gene function remains a bottleneck. Consequently, relatively few genes are well characterized, leaving the function of many as either hypothetical or entirely …
View article: Agr Quorum Sensing influences the Wood-Ljungdahl pathway in Clostridium autoethanogenum
Agr Quorum Sensing influences the Wood-Ljungdahl pathway in Clostridium autoethanogenum Open
View article: Application of transposon-insertion sequencing to determine gene essentiality in the acetogen <i>Clostridium autoethanogenum</i>
Application of transposon-insertion sequencing to determine gene essentiality in the acetogen <i>Clostridium autoethanogenum</i> Open
The majority of the genes present in bacterial genomes remain poorly characterised with up to one third of those that are protein encoding having no definitive function. Transposon insertion sequencing represents a high-throughput techniqu…
View article: A Gold Standard, CRISPR/Cas9-Based Complementation Strategy Reliant on 24 Nucleotide Bookmark Sequences
A Gold Standard, CRISPR/Cas9-Based Complementation Strategy Reliant on 24 Nucleotide Bookmark Sequences Open
Phenotypic complementation of gene knockouts is an essential step in establishing function. Here, we describe a simple strategy for ‘gold standard’ complementation in which the mutant allele is replaced in situ with a wild type (WT) allele…
View article: A Gold Standard, CRISPR/Cas9-Based Complementation Strategy Reliant on 24 Nucleotide Bookmark Sequences
A Gold Standard, CRISPR/Cas9-Based Complementation Strategy Reliant on 24 Nucleotide Bookmark Sequences Open
Phenotypic complementation of gene knock-outs is an essential step in establishing function. Here we describe a simple strategy for ‘gold standard’ complementation in which the mutant allele is replaced in situ with a wild type…
View article: Data for: A Novel Conjugal Donor Strain for Improved DNA transfer into Clostridium spp.
Data for: A Novel Conjugal Donor Strain for Improved DNA transfer into Clostridium spp. Open
Conjugation efficiencies comparing two donors into Clostridium autoethanogenum, C. difficile, and C. sporogenes.
View article: A novel conjugal donor strain for improved DNA transfer into Clostridium spp.
A novel conjugal donor strain for improved DNA transfer into Clostridium spp. Open
Clostridium encompasses species which are relevant to human and animal disease as well as species which have industrial potential, for instance, as producers of chemicals and fuels or as tumour delivery vehicles. Genetic manipulation of th…
View article: Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system
Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system Open
View article: Oxygen in patients with fibrotic interstitial lung disease: an international Delphi survey
Oxygen in patients with fibrotic interstitial lung disease: an international Delphi survey Open
Rationale Patients with fibrotic interstitial lung disease (ILD) frequently develop resting or exertional hypoxaemia. There is heterogeneity in clinical practice and a paucity of evidence guiding supplemental oxygen use in this patient pop…
View article: Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum
Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum Open
View article: On Methodologies of Resisting Testimonial Injustice
On Methodologies of Resisting Testimonial Injustice Open
Testimonial injustice, in its most pernicious form, subjects a speaker to identity-prejudicial deficits in the credibility that is rightly due their testimony. This paper compares two prominent accounts of testimonial injustice to determin…
View article: Advances in metabolic engineering in the microbial production of fuels and chemicals from C1 gas
Advances in metabolic engineering in the microbial production of fuels and chemicals from C1 gas Open
View article: A roadmap for gene system development in Clostridium
A roadmap for gene system development in Clostridium Open
View article: Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium
Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium Open
View article: Additional file 3: of Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium
Additional file 3: of Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium Open
Complete list of gene products with an alternative function to that previously described by Brown et al. (XLSX 101 kb)