Christopher J. Lengner
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View article: Forward genetic screening in engineered colorectal cancer organoids identifies regulators of metastasis
Forward genetic screening in engineered colorectal cancer organoids identifies regulators of metastasis Open
Metastatic outgrowth requires that cancer cells delaminate from the primary tumor, intravasate, survive in circulation, extravasate, migrate to, and proliferate at a distal site. Recurrent genetic drivers of metastasis remain elusive, sugg…
View article: Tissue-Specific and NF-kappaB-Independent Xist RNA Localization Patterns in Female Intestinal, Blood, and Muscle Progenitors
Tissue-Specific and NF-kappaB-Independent Xist RNA Localization Patterns in Female Intestinal, Blood, and Muscle Progenitors Open
X-Chromosome Inactivation (XCI), driven by the expression of Xist RNA and the enrichment of various repressive epigenetic marks, results in the formation of a mostly inactive X chromosome (Xi) to equalize X-linked gene expression between s…
View article: MAIT cell enrichment in Lynch syndrome is associated with immune surveillance and colorectal cancer risk
MAIT cell enrichment in Lynch syndrome is associated with immune surveillance and colorectal cancer risk Open
Tissue microenvironment characteristics associated with elevated risk of colorectal cancer (CRC) in Lynch syndrome (LS) are poorly characterized. We applied the multimodal single cell sequencing platform ExCITE-seq to define the colonic ce…
View article: Single-Cell RNA Sequencing and Inferred Protein Activity Analysis Reveal a Distinct Tumor Phenotype in Early-Onset Colorectal Cancer Patients
Single-Cell RNA Sequencing and Inferred Protein Activity Analysis Reveal a Distinct Tumor Phenotype in Early-Onset Colorectal Cancer Patients Open
Colorectal cancer (CRC) diagnosed before age 50 years (early-onset CRC, EO-CRC) is rising at an alarming rate, yet its molecular and microenvironmental drivers remain poorly understood. EO-CRC is highly heterogeneous, and while subtle diff…
View article: LIN28B-mediated PI3K/AKT pathway activation promotes metastasis in colorectal cancer models
LIN28B-mediated PI3K/AKT pathway activation promotes metastasis in colorectal cancer models Open
Colorectal cancer (CRC) remains a leading cause of cancer death because of metastatic spread. LIN28B is overexpressed in 30% of CRCs and promotes metastasis, yet its mechanisms remain unclear. In this study, we genetically modified CRC cel…
View article: An epigenetic basis for sustained inflammatory epithelial progenitor cell states in Crohn’s disease
An epigenetic basis for sustained inflammatory epithelial progenitor cell states in Crohn’s disease Open
SUMMARY Defining consequential differences in intestinal epithelial stem cells in healthy humans versus those with inflammatory bowel disease (Crohn’s disease and ulcerative colitis) is essential for the development of much needed therapie…
View article: Sox9 marks limbal stem cells and is required for asymmetric cell fate switch in the corneal epithelium
Sox9 marks limbal stem cells and is required for asymmetric cell fate switch in the corneal epithelium Open
Adult tissues with high cellular turnover require a balance between stem cell renewal and differentiation, yet the mechanisms underlying this equilibrium are unclear. The cornea exhibits a polarized lateral flow of progenitors from the per…
View article: Sox9 marks limbal stem cells and is required for asymmetric cell fate switch in the corneal epithelium
Sox9 marks limbal stem cells and is required for asymmetric cell fate switch in the corneal epithelium Open
Adult tissues with high cellular turnover require a balance between stem cell renewal and differentiation, yet the mechanisms underlying this equilibrium are unclear. The cornea exhibits a polarized lateral flow of progenitors from the per…
View article: Early Inflammation and Interferon Signaling Direct Enhanced Intestinal Crypt Regeneration after Proton FLASH Radiotherapy
Early Inflammation and Interferon Signaling Direct Enhanced Intestinal Crypt Regeneration after Proton FLASH Radiotherapy Open
Ultra-high dose rate ("FLASH") radiotherapy (>40-60 Gy/s) is a promising new radiation modality currently in human clinical trials. Previous studies showed that FLASH proton radiotherapy (FR) improves toxicity of normal tissues compared to…
View article: Sox9 marks limbal stem cells and is required for asymmetric cell fate switch in the corneal epithelium
Sox9 marks limbal stem cells and is required for asymmetric cell fate switch in the corneal epithelium Open
Adult tissues with high cellular turnover require a balance between stem cell renewal and differentiation, yet the mechanisms underlying this equilibrium are unclear. The cornea exhibits a polarized lateral flow of progenitors from the per…
View article: Musashi-2 (MSI2) regulation of DNA damage response in lung cancer
Musashi-2 (MSI2) regulation of DNA damage response in lung cancer Open
Lung cancer is one of the most common types of cancer worldwide. Non-small cell lung cancer (NSCLC), typically caused by KRAS and TP53 driver mutations, represents the majority of all new lung cancer diagnoses. Overexpression of the RNA-bi…
View article: Mapping and modeling human colorectal carcinoma interactions with the tumor microenvironment
Mapping and modeling human colorectal carcinoma interactions with the tumor microenvironment Open
This dataset can be interactively explored with VisCello (https://github.com/qinzhu/VisCello). Please install VisCello and use e.g. "cello("path_to/hcc_main_cello")" to load the human colon cancer single cell dataset.
View article: Mapping and modeling human colorectal carcinoma interactions with the tumor microenvironment
Mapping and modeling human colorectal carcinoma interactions with the tumor microenvironment Open
This dataset can be interactively explored with VisCello (https://github.com/qinzhu/VisCello). Please install VisCello and use e.g. "cello("path_to/hcc_main_cello")" to load the human colon cancer single cell dataset.
View article: <i>APC</i> and <i>P53</i> mutations synergise to create a therapeutic vulnerability to NOTUM inhibition in advanced colorectal cancer
<i>APC</i> and <i>P53</i> mutations synergise to create a therapeutic vulnerability to NOTUM inhibition in advanced colorectal cancer Open
Objective Colorectal cancer (CRC) is a leading cause of cancer-related deaths, with the majority of cases initiated by inactivation of the APC tumour suppressor. This results in the constitutive activation of canonical WNT pathway transcri…
View article: Forward genetic screening in engineered colorectal cancer organoids identifies novel regulators of metastasis
Forward genetic screening in engineered colorectal cancer organoids identifies novel regulators of metastasis Open
Summary Cancer is the second leading cause of death globally, due primarily to metastatic dissemination and colonization of distal sites. Recurrent genetic drivers of metastasis are elusive, suggesting that, unlike the stereotyped mutation…
View article: Musashi Exerts Control of Gonadotrope Target mRNA Translation During the Mouse Estrous Cycle
Musashi Exerts Control of Gonadotrope Target mRNA Translation During the Mouse Estrous Cycle Open
The anterior pituitary controls key biological processes, including growth, metabolism, reproduction, and stress responses through distinct cell types that each secrete specific hormones. The anterior pituitary cells show a remarkable leve…
View article: The Tumor Suppressor Adenomatous Polyposis Coli (apc) Is Required for Neural Crest-Dependent Craniofacial Development in Zebrafish
The Tumor Suppressor Adenomatous Polyposis Coli (apc) Is Required for Neural Crest-Dependent Craniofacial Development in Zebrafish Open
Neural crest (NC) is a unique vertebrate cell type arising from the border of the neural plate and epidermis that gives rise to diverse tissues along the entire body axis. Roberto Mayor and colleagues have made major contributions to our u…
View article: LIN28B promotes cell invasion and colorectal cancer metastasis via CLDN1 and NOTCH3
LIN28B promotes cell invasion and colorectal cancer metastasis via CLDN1 and NOTCH3 Open
The RNA-binding protein LIN28B is overexpressed in over 30% of patients with colorectal cancer (CRC) and is associated with poor prognosis. In the present study, we unraveled a potentially novel mechanism by which LIN28B regulates colonic …
View article: Musashi-2 (MSI2) regulation of DNA damage response in lung cancer
Musashi-2 (MSI2) regulation of DNA damage response in lung cancer Open
Lung cancer is one of the most common types of cancers worldwide. Non-small cell lung cancer (NSCLC), typically caused by KRAS and TP53 driver mutations, represents the majority of all new lung cancer diagnoses. Overexpression of the RNA-b…
View article: Data from MYC Hyperactivates Wnt Signaling in <i>APC</i>/<i>CTNNB1</i>-Mutated Colorectal Cancer Cells through miR-92a–Dependent Repression of <i>DKK3</i>
Data from MYC Hyperactivates Wnt Signaling in <i>APC</i>/<i>CTNNB1</i>-Mutated Colorectal Cancer Cells through miR-92a–Dependent Repression of <i>DKK3</i> Open
Activation of Wnt signaling is among the earliest events in colon cancer development. It is achieved either via activating mutations in the CTNNB1 gene encoding β-catenin, the key transcription factor in the Wnt pathway, or most commonly b…
View article: Supplementary Figures 1 and 2 from MYC Hyperactivates Wnt Signaling in <i>APC</i>/<i>CTNNB1</i>-Mutated Colorectal Cancer Cells through miR-92a–Dependent Repression of <i>DKK3</i>
Supplementary Figures 1 and 2 from MYC Hyperactivates Wnt Signaling in <i>APC</i>/<i>CTNNB1</i>-Mutated Colorectal Cancer Cells through miR-92a–Dependent Repression of <i>DKK3</i> Open
S1. DKK3 and Wnt signaling in CRC cells. S2. Derivation of murine colorectal tumoroids.
View article: Data from MYC Hyperactivates Wnt Signaling in <i>APC</i>/<i>CTNNB1</i>-Mutated Colorectal Cancer Cells through miR-92a–Dependent Repression of <i>DKK3</i>
Data from MYC Hyperactivates Wnt Signaling in <i>APC</i>/<i>CTNNB1</i>-Mutated Colorectal Cancer Cells through miR-92a–Dependent Repression of <i>DKK3</i> Open
Activation of Wnt signaling is among the earliest events in colon cancer development. It is achieved either via activating mutations in the CTNNB1 gene encoding β-catenin, the key transcription factor in the Wnt pathway, or most commonly b…
View article: Supplementary Figures 1 and 2 from MYC Hyperactivates Wnt Signaling in <i>APC</i>/<i>CTNNB1</i>-Mutated Colorectal Cancer Cells through miR-92a–Dependent Repression of <i>DKK3</i>
Supplementary Figures 1 and 2 from MYC Hyperactivates Wnt Signaling in <i>APC</i>/<i>CTNNB1</i>-Mutated Colorectal Cancer Cells through miR-92a–Dependent Repression of <i>DKK3</i> Open
S1. DKK3 and Wnt signaling in CRC cells. S2. Derivation of murine colorectal tumoroids.
View article: Supplementary Data from Dll1-Mediated Notch Signaling Drives Tumor Cell Cross-talk with Cancer-Associated Fibroblasts to Promote Radioresistance in Breast Cancer
Supplementary Data from Dll1-Mediated Notch Signaling Drives Tumor Cell Cross-talk with Cancer-Associated Fibroblasts to Promote Radioresistance in Breast Cancer Open
Supplementary Data from Dll1-Mediated Notch Signaling Drives Tumor Cell Cross-talk with Cancer-Associated Fibroblasts to Promote Radioresistance in Breast Cancer
View article: Supplementary Data from Dll1-Mediated Notch Signaling Drives Tumor Cell Cross-talk with Cancer-Associated Fibroblasts to Promote Radioresistance in Breast Cancer
Supplementary Data from Dll1-Mediated Notch Signaling Drives Tumor Cell Cross-talk with Cancer-Associated Fibroblasts to Promote Radioresistance in Breast Cancer Open
Supplementary Data from Dll1-Mediated Notch Signaling Drives Tumor Cell Cross-talk with Cancer-Associated Fibroblasts to Promote Radioresistance in Breast Cancer