Christopher J. Obara
YOU?
Author Swipe
View article: Mitochondrial complexity is regulated at ER-mitochondria contact sites via PDZD8-FKBP8 tethering
Mitochondrial complexity is regulated at ER-mitochondria contact sites via PDZD8-FKBP8 tethering Open
Mitochondria-ER membrane contact sites (MERCS) represent a fundamental ultrastructural feature underlying unique biochemistry and physiology in eukaryotic cells. The ER protein PDZD8 is required for the formation of MERCS in many cell type…
View article: Motion of VAPB molecules reveals ER–mitochondria contact site subdomains
Motion of VAPB molecules reveals ER–mitochondria contact site subdomains Open
To coordinate cellular physiology, eukaryotic cells rely on the rapid exchange of molecules at specialized organelle–organelle contact sites 1,2 . Endoplasmic reticulum–mitochondrial contact sites (ERMCSs) are particularly vital communicat…
View article: VAPB + PTPIP51 Dataset (Obara + Nixon-Abell et al., 2023)
VAPB + PTPIP51 Dataset (Obara + Nixon-Abell et al., 2023) Open
WT VAPB trajectories and raw images from cells overexpressing PTPIP51 that are used for the work generated in Obara + Nixon-Abell et al., 2023.
View article: VAPB-HBSS Dataset (Obara + Nixon-Abell et al., 2023) v.1
VAPB-HBSS Dataset (Obara + Nixon-Abell et al., 2023) v.1 Open
WT VAPB trajectories and raw imaging data from cells treated with 8 hours of HBSS starvation that are used for the work generated in Obara + Nixon-Abell et al., 2023.
View article: VAPB-HBSS Dataset (Obara + Nixon-Abell et al., 2023) v.1
VAPB-HBSS Dataset (Obara + Nixon-Abell et al., 2023) v.1 Open
WT VAPB trajectories and raw imaging data from cells treated with 8 hours of HBSS starvation that are used for the work generated in Obara + Nixon-Abell et al., 2023.
View article: VAPB Dataset (Obara + Nixon-Abell et al., 2023) v.1
VAPB Dataset (Obara + Nixon-Abell et al., 2023) v.1 Open
WT VAPB trajectories in 24 cells that are used for the work generated in Obara + Nixon-Abell et al., 2023.
View article: VAPB + PTPIP51 Dataset (Obara + Nixon-Abell et al., 2023)
VAPB + PTPIP51 Dataset (Obara + Nixon-Abell et al., 2023) Open
WT VAPB trajectories and raw images from cells overexpressing PTPIP51 that are used for the work generated in Obara + Nixon-Abell et al., 2023.
View article: FIB-SEM Dataset (Obara + Nixon-Abell et al., 2023)
FIB-SEM Dataset (Obara + Nixon-Abell et al., 2023) Open
Analyzed portions of FIB-SEM volumes used for analysis in Obara + Nixon-Abell et al., 2023. Full datasets are available upon request from authors, they are just too large to easily store in this medium. Email Jennifer Lippincott-Schwartz a…
View article: VAPB Dataset (Obara + Nixon-Abell et al., 2023) v.1
VAPB Dataset (Obara + Nixon-Abell et al., 2023) v.1 Open
WT VAPB trajectories and raw images that are used for the work generated in Obara + Nixon-Abell et al., 2023.
View article: P56S VAPB Dataset (Obara + Nixon-Abell et al., 2023)
P56S VAPB Dataset (Obara + Nixon-Abell et al., 2023) Open
P56S VAPB trajectories and raw images from cells that are used for the work generated in Obara + Nixon-Abell et al., 2023.
View article: VAPB Dataset (Obara + Nixon-Abell et al., 2023) v.1
VAPB Dataset (Obara + Nixon-Abell et al., 2023) v.1 Open
WT VAPB trajectories and raw images that are used for the work generated in Obara + Nixon-Abell et al., 2023.
View article: P56S VAPB Dataset (Obara + Nixon-Abell et al., 2023)
P56S VAPB Dataset (Obara + Nixon-Abell et al., 2023) Open
P56S VAPB trajectories and raw images from cells that are used for the work generated in Obara + Nixon-Abell et al., 2023.
View article: FIB-SEM Dataset (Obara + Nixon-Abell et al., 2023)
FIB-SEM Dataset (Obara + Nixon-Abell et al., 2023) Open
Analyzed portions of FIB-SEM volumes used for analysis in Obara + Nixon-Abell et al., 2023. Full datasets are available upon request from authors, they are just too large to easily store in this medium. Email Jennifer Lippincott-Schwartz a…
View article: PDZD8-FKBP8 tethering complex at ER-mitochondria contact sites regulates mitochondrial complexity
PDZD8-FKBP8 tethering complex at ER-mitochondria contact sites regulates mitochondrial complexity Open
Mitochondria-ER membrane contact sites (MERCS) represent a fundamental ultrastructural feature underlying unique biochemistry and physiology in eukaryotic cells. The ER protein PDZD8 is required for the formation of MERCS in many cell type…
View article: Simultaneous photoactivation and high-speed structural tracking reveal diffusion-dominated motion in the endoplasmic reticulum
Simultaneous photoactivation and high-speed structural tracking reveal diffusion-dominated motion in the endoplasmic reticulum Open
The endoplasmic reticulum (ER) is a structurally complex, membrane-enclosed compartment that stretches from the nuclear envelope to the extreme periphery of eukaryotic cells. The organelle is crucial for numerous distinct cellular processe…
View article: Fast and sensitive GCaMP calcium indicators for imaging neural populations
Fast and sensitive GCaMP calcium indicators for imaging neural populations Open
Calcium imaging with protein-based indicators 1,2 is widely used to follow neural activity in intact nervous systems, but current protein sensors report neural activity at timescales much slower than electrical signalling and are limited b…
View article: Structural Diversity within the Endoplasmic Reticulum—From the Microscale to the Nanoscale
Structural Diversity within the Endoplasmic Reticulum—From the Microscale to the Nanoscale Open
The endoplasmic reticulum (ER) is a continuous, highly dynamic membrane compartment that is crucial for numerous basic cellular functions. The ER stretches from the nuclear envelope to the outer periphery of all living eukaryotic cells. Th…
View article: Motion of single molecular tethers reveals dynamic subdomains at ER-mitochondria contact sites
Motion of single molecular tethers reveals dynamic subdomains at ER-mitochondria contact sites Open
To coordinate cellular physiology, eukaryotic cells rely on the inter-organelle transfer of molecules at specialized organelle-organelle contact sites 1,2 . Endoplasmic reticulum-mitochondria contact sites (ERMCSs) are particularly vital c…
View article: Unraveling trajectories of diffusive particles on networks
Unraveling trajectories of diffusive particles on networks Open
The analysis of single-particle trajectories plays an important role in elucidating dynamics within complex environments such as those found in living cells. However, the characterization of intracellular particle motion is often confounde…
View article: Unraveling Single-Particle Trajectories Confined in Tubular Networks
Unraveling Single-Particle Trajectories Confined in Tubular Networks Open
The analysis of single particle trajectories plays an important role in elucidating dynamics within complex environments such as those found in living cells. However, the characterization of intracellular particle motion is often confounde…
View article: Fast and sensitive GCaMP calcium indicators for imaging neural populations
Fast and sensitive GCaMP calcium indicators for imaging neural populations Open
Calcium imaging with protein-based indicators is widely used to follow neural activity in intact nervous systems. The popular GCaMP indicators are based on the calcium-binding protein calmodulin and the RS20 peptide. These sensors report n…
View article: Fast and sensitive GCaMP calcium indicators for imaging neural populations
Fast and sensitive GCaMP calcium indicators for imaging neural populations Open
Calcium imaging with protein-based indicators is widely used to follow neural activity in intact nervous systems. The popular GCaMP indicators are based on the calcium-binding protein calmodulin and the RS20 peptide. These sensors report n…
View article: Deep learning enables fast and dense single-molecule localization with high accuracy
Deep learning enables fast and dense single-molecule localization with high accuracy Open
Single-molecule localization microscopy (SMLM) has had remarkable success in imaging cellular structures with nanometer resolution, but the need for activating only single isolated emitters limits imaging speed and labeling density. Here, …
View article: Teaching deep neural networks to localize single molecules for super-resolution microscopy
Teaching deep neural networks to localize single molecules for super-resolution microscopy Open
Single-molecule localization fluorescence microscopy constructs super-resolution images by sequential imaging and computational localization of sparsely activated fluorophores. Accurate and efficient fluorophore localization algorithms are…