P. Christopher LaRosa
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View article: Cloning of a polycistronic cDNA from tomato encoding γ-glutamyl kinase and γ-glutamyl phosphate reductase
Cloning of a polycistronic cDNA from tomato encoding γ-glutamyl kinase and γ-glutamyl phosphate reductase Open
We isolated from a tomato cDNA library the tom PRO1 locus, which encodes γ-glutamyl kinase (GK) and γ-glutamyl phosphate reductase (GPR). This locus is unusual among eukaryotic genetic elements because it contains two open reading frames, …
View article: Osmotin Gene Expression Is Posttranscriptionally Regulated
Osmotin Gene Expression Is Posttranscriptionally Regulated Open
Accumulation of both osmotin mRNA and osmotin protein in tissues of tobacco (Nicotiana tabacum L. var Wisconsin 38) is subject to complex developmental control. Osmotin was found to be most abundant in tobacco roots and in tissues of the o…
View article: Elevated Accumulation of Proline in NaCl-Adapted Tobacco Cells Is Not Due to Altered Δ<sup>1</sup>-Pyrroline-5-Carboxylate Reductase
Elevated Accumulation of Proline in NaCl-Adapted Tobacco Cells Is Not Due to Altered Δ<sup>1</sup>-Pyrroline-5-Carboxylate Reductase Open
Tobacco (Nicotiana tabacum L. var Wisconsin 38) cells that are adapted to 428 millimolar NaCl accumulate proline mainly due to increased synthesis from glutamate. These cells were used to evaluate the possible role of Delta(1)-pyrroline-5-…
View article: Stable NaCl Tolerance of Tobacco Cells Is Associated with Enhanced Accumulation of Osmotin
Stable NaCl Tolerance of Tobacco Cells Is Associated with Enhanced Accumulation of Osmotin Open
Osmotin is a major protein which accumulates in tobacco cells (Nicotiana tabacum L. var Wisconsin 38) adapted to low water potentials. Quantitation of osmotin levels by immunoblots indicated that cells adapted to 428 millimolar NaCl contai…
View article: Abscisic Acid Stimulated Osmotic Adjustment and Its Involvement in Adaptation of Tobacco Cells to NaCl
Abscisic Acid Stimulated Osmotic Adjustment and Its Involvement in Adaptation of Tobacco Cells to NaCl Open
Osmotic adjustment of cultured tobacco (Nicotiana tabacum L. var Wisconsin 38) cells was stimulated by 10 micromolar (+/-) abscisic acid (ABA) during adaptation to water deficit imposed by various solutes including NaCl, KCl, K(2)SO(4), Na…
View article: Hormonal regulation of protein synthesis associated with salt tolerance in plant cells
Hormonal regulation of protein synthesis associated with salt tolerance in plant cells Open
Cultured tobacco cells ( Nicotiana tabacum L. cv. Wisconsin 38) synthesize a predominant 26-kDa protein upon exposure to abscisic acid (ABA). ABA also accelerates the rate of adaptation of unadapted cells to NaCl stress. The ABA-induced 26…
View article: Abscisic Acid Accelerates Adaptation of Cultured Tobacco Cells to Salt
Abscisic Acid Accelerates Adaptation of Cultured Tobacco Cells to Salt Open
Adaptation of tobacco (Nicotiana tabacum L. var Wisconsin 38) cells to NaCl was accelerated by (+/-) abscisic acid (ABA). In medium with 10 grams per liter NaCl, ABA stimulated the growth of cells not grown in medium with NaCl (unadapted, …