Christopher M. Yip
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PGAM5 cleavage and oligomerization equilibrates mitochondrial dynamics under stress by regulating DRP1 function Open
Mitochondrial dynamics relies on the function of dynamin family GTPase proteins including mitofusin 1 (MFN1), mitofusin 2 (MFN2) and dynamin-related protein 1 (DRP1; also known as DNM1L). The mitochondrial phosphatase phosphoglycerate muta…
Morphological heterogeneity of human astrocytes in cerebral organoids Open
Astrocytes are a type of glial cell in the central nervous system responsible for modulating synaptic transmissions, tissue repair, maintaining homeostasis, and are therefore implicated in many neurological diseases. Human cortical astrocy…
Line-scan imaging for real-time phenotypic screening of <i>C. elegans</i> Open
Phenotypic screens of living organisms often require real-time, high spatial resolution imaging. We report here on the design and application of a line-scan imaging platform that enables bright-field imaging of live nematodes using a 96-we…
Harnessing Microbes to Weather Native Silicates in Agricultural Soils for Scalable Carbon Dioxide Removal Open
Anthropogenic carbon emissions contribute significantly to the greenhouse effect, resulting in global warming and climate change. Thus, addressing this critical issue requires innovative and comprehensive solutions. Silicate weathering mod…
View article: The TSC22D, WNK, and NRBP gene families exhibit functional buffering and evolved with Metazoa for cell volume regulation
The TSC22D, WNK, and NRBP gene families exhibit functional buffering and evolved with Metazoa for cell volume regulation Open
The ability to sense and respond to osmotic fluctuations is critical for the maintenance of cellular integrity. We used gene co-essentiality analysis to identify an unappreciated relationship between TSC22D2, WNK1, and NRBP1 in regulating …
Microbially-enhanced silicate weathering in agricultural soils to remove carbon dioxide Open
Silicate weathering moderates atmospheric CO 2 levels over geologic time but proceeds naturally at rates too slow to substantially mitigate anthropogenic emissions. Microorganisms can accelerate silicate weathering and may thereby augment …
Microbially-enhanced silicate weathering in agricultural soils to remove carbon dioxide Open
Silicate weathering moderates atmospheric CO 2 levels over geologic time but proceeds naturally at rates too slow to substantially mitigate anthropogenic emissions. Microorganisms can accelerate silicate weathering and may thereby augment …
View article: Salmonella exploits membrane reservoirs for invasion of host cells
Salmonella exploits membrane reservoirs for invasion of host cells Open
Salmonella utilizes a type 3 secretion system to translocate virulence proteins (effectors) into host cells during infection 1 . The effectors modulate host cell machinery to drive uptake of the bacteria into vacuoles, where they can estab…
View article: <i>TSC22D</i>,<i>WNK</i>and<i>NRBP</i>gene families exhibit functional buffering and evolved with Metazoa for macromolecular crowd sensing
<i>TSC22D</i>,<i>WNK</i>and<i>NRBP</i>gene families exhibit functional buffering and evolved with Metazoa for macromolecular crowd sensing Open
SUMMARY The ability to sense and respond to osmotic fluctuations is critical for the maintenance of cellular integrity. Myriad redundancies have evolved across all facets of osmosensing in metazoans, including among water and ion transport…
PGAM5 is an MFN2 phosphatase that plays an essential role in the regulation of mitochondrial dynamics Open
Mitochondrial morphology is regulated by the post-translational modifications of the dynamin family GTPase proteins including mitofusin 1 (MFN1), MFN2, and dynamin-related protein 1 (DRP1). Mitochondrial phosphatase phosphoglycerate mutase…
Islet-on-a-chip device reveals first phase glucose-stimulated respiration is substrate limited by glycolysis independent of Ca2+ activity. Open
Pancreatic islets respond metabolically to glucose by closing KATP channels resulting in Ca2+-influx and insulin secretion. Previous work has revealed the importance of glycolytic flux in triggering insulin secretion. However, it is unclea…
Monitoring non-specific adsorption at solid–liquid interfaces by supercritical angle fluorescence microscopy Open
Supercritical angle fluorescence (SAF) microscopy is a novel imaging tool based on the use of distance-dependent fluorophore emission patterns to provide accurate locations of fluorophores relative to a surface. This technique has been ext…
Targeting Apollo-NADP <sup>+</sup> to Image NADPH Generation in Pancreatic Beta-Cell Organelles Open
NADPH/NADP+ redox state supports numerous reactions related to cell growth and survival; yet the full impact is difficult to appreciate due to organelle compartmentalization of NADPH and NADP+. To study glucose-stimulated NADPH production …
Probing both sides of the story Open
Microbial communities are found throughout the biosphere, from human guts to glaciers, from soil to activated sludge. Understanding the statistical properties of such diverse communities can pave the way to elucidate the common mechanisms …
Correlated STORM-homoFRET imaging reveals highly heterogeneous membrane receptor structures Open
Mapping the self-organization and spatial distribution of membrane proteins is key to understanding their function. Developing methods that can provide insight into correlations between membrane protein colocalization and interactions is c…
A Practical Approach for Optimizing Off-axis Telecentric Digital Holographic Microscope Design Open
Digital holographic microscopy (DHM) has become an attractive imaging tool for the analysis of living cells and histological tissues. The telecentric DHM (TDHM) is a configuration of DHM that lightens the computation load with a priori abe…
Monitoring Non-Specific Adsorption at Solid-Liquid Interfaces by Supercritical Angle Fluorescence Microscopy Open
Supercritical angle fluorescence (SAF) microscopy is a novel imaging tool based on the use of distance-dependent fluorophore emission patterns to provide accurate locations of fluorophores relative to a surface. This technique has been use…
Design of a versatile microfluidic device for imaging precision-cut-tissue slices Open
Precision-cut-tissues (PCTs), which preserve many aspects of a tissue’s microenvironment, are typically imaged using conventional sample dishes and chambers. These can require large amounts of reagent and, when used for flow-through experi…