Christopher Mark Skopnik
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View article: Lack of evidence for viral etiology in symptomatic chronic kidney disease of unknown etiology (CKDu) in Sri Lanka
Lack of evidence for viral etiology in symptomatic chronic kidney disease of unknown etiology (CKDu) in Sri Lanka Open
Not applicable.
View article: #758 Deep phenotyping of urinary cells with cytometry by time of flight reveals CD8+CD38+ T cells as biomarker to detect T cell-mediated rejection
#758 Deep phenotyping of urinary cells with cytometry by time of flight reveals CD8+CD38+ T cells as biomarker to detect T cell-mediated rejection Open
Background and Aims Non-invasive monitoring and early detection of rejection are of high priority in kidney transplantation. In our previous work, we established urine flow cytometry (FC) as tool for non-invasive kidney transplant monitori…
View article: #1349 Flow-cytometric quantification of urine kidney epithelial cells specifically reflects tubular damage in acute kidney diseases
#1349 Flow-cytometric quantification of urine kidney epithelial cells specifically reflects tubular damage in acute kidney diseases Open
Background and Aims Despite tubular injury being one of the main mechanisms driving acute kidney injury (AKI), clinicians still have a limited diagnostic repertoire to precisely monitor damage to tubular epithelial cells (TEC). In our prev…
View article: Viability-Compatible Preservation for Mass Cytometry Using Cisplatin Pulse Quenching and Gradual Formaldehyde Release
Viability-Compatible Preservation for Mass Cytometry Using Cisplatin Pulse Quenching and Gradual Formaldehyde Release Open
Objective Mass cytometry (CyTOF) enables high-dimensional single-cell profiling, but especially for non-blood derived samples relies on immediate processing, limiting its application to fragile, low-yield clinical specimens such as urine. …
View article: Flow-Cytometric Quantification of Urine Kidney Epithelial Cells Specifically Reflects Tubular Damage in Acute Kidney Diseases
Flow-Cytometric Quantification of Urine Kidney Epithelial Cells Specifically Reflects Tubular Damage in Acute Kidney Diseases Open
We propose that urinary CD227/CD326+ TEC count is a specific, noninvasive marker for tubular injury in AKI. Our protocol provides a basis for a deeper phenotypic analysis of urinary TECs.
View article: Issue Information
Issue Information Open
View article: Physiological and Pathophysiological Roles of IgM Fc Receptor (FcµR) Isoforms
Physiological and Pathophysiological Roles of IgM Fc Receptor (FcµR) Isoforms Open
IgM is the first antibody to emerge during phylogeny, ontogeny, and immune responses and serves as a first line of defense. Effector proteins interacting with the Fc portion of IgM, such as complement and its receptors, have been extensive…
View article: Addition of formaldehyde releaser imidazolidinyl urea and <scp>MOPS</scp> buffer to urine samples enables delayed processing for flow cytometric analysis of urinary cells: A simple, two step conservation method of urinary cells for flow cytometry
Addition of formaldehyde releaser imidazolidinyl urea and <span>MOPS</span> buffer to urine samples enables delayed processing for flow cytometric analysis of urinary cells: A simple, two step conservation method of urinary cells for flow cytometry Open
Introduction Kidney diseases are a major health concern worldwide. Currently there is a large unmet need for novel biomarkers to non‐invasively diagnose and monitor kidney diseases. Urinary cells are promising biomarkers and their analysis…
View article: Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury
Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury Open
View article: Urinary single-cell sequencing captures kidney injury and repair processes in human acute kidney injury
Urinary single-cell sequencing captures kidney injury and repair processes in human acute kidney injury Open
View article: Urinary CD8+HLA-DR+ T Cell Abundance Non-invasively Predicts Kidney Transplant Rejection
Urinary CD8+HLA-DR+ T Cell Abundance Non-invasively Predicts Kidney Transplant Rejection Open
Early detection of kidney transplant (KT) rejection remains a challenge in patient care. Non-invasive biomarkers hold high potential to detect rejection, adjust immunosuppression, and monitor KT patients. So far, no approach has fully sati…
View article: Questioning whether IgM Fc receptor (FcµR) is expressed by innate immune cells
Questioning whether IgM Fc receptor (FcµR) is expressed by innate immune cells Open
View article: Addition of formaldehyde releaser imidazolidinyl urea and MOPS buffer to urine samples enables delayed processing for flow cytometric analysis of urinary cells
Addition of formaldehyde releaser imidazolidinyl urea and MOPS buffer to urine samples enables delayed processing for flow cytometric analysis of urinary cells Open
Kidney diseases are a major health concern worldwide. Currently there is a large unmet need for novel biomarkers to non-invasively diagnose and monitor kidney diseases. Urinary cells are promising biomarkers and their analysis by flow cyto…
View article: Urinary single-cell sequencing captures intrarenal injury and repair processes in human acute kidney injury
Urinary single-cell sequencing captures intrarenal injury and repair processes in human acute kidney injury Open
Acute kidney injury (AKI) is a major health issue, the outcome of which depends primarily on damage and reparative processes of tubular epithelial cells (TEC). Mechanisms underlying AKI remain incompletely understood, specific therapies ar…
View article: Monitoring tubular epithelial cell damage in AKI via urine flow cytometry
Monitoring tubular epithelial cell damage in AKI via urine flow cytometry Open
Introduction Acute kidney injury (AKI) is associated with significant morbidity and mortality. The diagnosis is currently based on urine output and serum creatinine and there is a lack of biomarkers that directly reflect tubular damage. He…
View article: Additional file 9 of Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury
Additional file 9 of Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury Open
Additional file 9: Table S8. Results from transcriptional regulation analysis on the joint marker genes of the New 1-4 cell states.
View article: Additional file 8 of Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury
Additional file 8 of Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury Open
Additional file 8: Table S7. Marker gene comparison between AKI-induced cell states of PT, TAL and DCT.
View article: Additional file 5 of Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury
Additional file 5 of Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury Open
Additional file 5: Table S4. Results from differential gene expression analysis COVID vs. non-COVID AKI.
View article: Additional file 3 of Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury
Additional file 3 of Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury Open
Additional file 3: Table S2. Results from differential gene expression analysis control versus AKI.
View article: Additional file 2 of Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury
Additional file 2 of Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury Open
Additional file 2: Table S1. Clinical and histopathological information on included individuals.
View article: Additional file 7 of Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury
Additional file 7 of Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury Open
Additional file 7: Table S6. Marker genes of all identified sub cell types and cell states.
View article: Additional file 6 of Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury
Additional file 6 of Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury Open
Additional file 6: Table S5. Results from pathway enrichment analyses COVID AKI versus non-COVID AKI.
View article: Additional file 4 of Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury
Additional file 4 of Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury Open
Additional file 4: Table S3. Results from pathway enrichment analyses AKI versus controls.
View article: Transcriptomic responses of the human kidney to acute injury at single cell resolution
Transcriptomic responses of the human kidney to acute injury at single cell resolution Open
Background Acute kidney injury (AKI) occurs frequently in critically ill patients and is associated with adverse outcomes. Cellular mechanisms underlying AKI and kidney cell responses to injury remain incompletely understood. Methods We pe…
View article: Differences between Human and Mouse IgM Fc Receptor (FcµR)
Differences between Human and Mouse IgM Fc Receptor (FcµR) Open
Both non-immune “natural” and antigen-induced “immune” IgM are important for protection against pathogens and for regulation of immune responses to self-antigens. Since the bona fide IgM Fc receptor (FcµR) was identified in humans by a fun…
View article: Identification of Amino Acid Residues in Human IgM Fc Receptor (FcµR) Critical for IgM Binding
Identification of Amino Acid Residues in Human IgM Fc Receptor (FcµR) Critical for IgM Binding Open
Both non-immune “natural” and antigen-induced “immune” IgM are important for protection against infections and for regulation of immune responses to self-antigens. The roles of its Fc receptor (FcµR) in these IgM effector functions have be…
View article: Antigen‐driven PD‐1 <sup>+</sup> <i>TOX</i> <sup>+</sup> <i>BHLHE40</i> <sup>+</sup> and PD‐1 <sup>+</sup> <i>TOX</i> <sup>+</sup> <i>EOMES</i> <sup>+</sup> T lymphocytes regulate juvenile idiopathic arthritis <i>in situ</i>
Antigen‐driven PD‐1 <sup>+</sup> <i>TOX</i> <sup>+</sup> <i>BHLHE40</i> <sup>+</sup> and PD‐1 <sup>+</sup> <i>TOX</i> <sup>+</sup> <i>EOMES</i> <sup>+</sup> T lymphocytes regulate juvenile idiopathic arthritis <i>in situ</i> Open
T lymphocytes accumulate in inflamed tissues of patients with chronic inflammatory diseases (CIDs) and express pro‐inflammatory cytokines upon re‐stimulation in vitro . Further, a significant genetic linkage to MHC genes suggests that T ly…
View article: Author response for "Antigen‐driven PD‐1 <sup>+</sup> <i>TOX</i> <sup>+</sup> <i>BHLHE40</i> <sup>+</sup> and PD‐1 <sup>+</sup> <i>TOX</i> <sup>+</sup> <i>EOMES</i> <sup>+</sup> T lymphocytes regulate juvenile idiopathic arthritis <i>in situ</i>"
Author response for "Antigen‐driven PD‐1 <sup>+</sup> <i>TOX</i> <sup>+</sup> <i>BHLHE40</i> <sup>+</sup> and PD‐1 <sup>+</sup> <i>TOX</i> <sup>+</sup> <i>EOMES</i> <sup>+</sup> T lymphocytes regulate juvenile idiopathic arthritis <i>in situ</i>" Open
View article: Antigen-driven PD-1<sup>+</sup><i>TOX</i><sup>+</sup><i>EOMES</i><sup>+</sup>and PD-1<sup>+</sup><i>TOX</i><sup>+</sup><i>BHLHE40</i><sup>+</sup>synovial T lymphocytes regulate chronic inflammation<i>in situ</i>
Antigen-driven PD-1<sup>+</sup><i>TOX</i><sup>+</sup><i>EOMES</i><sup>+</sup>and PD-1<sup>+</sup><i>TOX</i><sup>+</sup><i>BHLHE40</i><sup>+</sup>synovial T lymphocytes regulate chronic inflammation<i>in situ</i> Open
Introduction/Abstract T lymphocytes accumulate in inflamed tissues of patients with chronic inflammatory diseases (CIDs) and express pro-inflammatory cytokines upon re-stimulation in vitro 1–29 . Further, a significant genetic linkage to M…