Christopher Schächner
YOU?
Author Swipe
View article: Quantitative chest computed tomography: regional differences in dual-energy-derived virtual vs. true non-contrast scans
Quantitative chest computed tomography: regional differences in dual-energy-derived virtual vs. true non-contrast scans Open
Although slight regional differences were observed, quantitative analysis can be reliably performed using VNC images.
View article: Establishment of closed 35S ribosomal RNA gene chromatin in stationary <i>Saccharomyces cerevisiae</i> cells
Establishment of closed 35S ribosomal RNA gene chromatin in stationary <i>Saccharomyces cerevisiae</i> cells Open
As a first step in eukaryotic ribosome biogenesis RNA polymerase (Pol) I synthesizes a large ribosomal RNA (rRNA) precursor from multicopy rRNA gene loci. This process is essential for cellular growth and regulated in response to the cell’…
View article: Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryotes
Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryotes Open
In growing eukaryotic cells, nuclear ribosomal (r)RNA synthesis by RNA polymerase (RNAP) I accounts for the vast majority of cellular transcription. This high output is achieved by the presence of multiple copies of rRNA genes in eukaryoti…
View article: Analysis of Yeast RNAP I Transcription of Nucleosomal Templates In Vitro
Analysis of Yeast RNAP I Transcription of Nucleosomal Templates In Vitro Open
Nuclear eukaryotic RNA polymerases (RNAPs) transcribe a chromatin template in vivo. Since the basic unit of chromatin, the nucleosome, renders the DNA largely inaccessible, RNAPs have to overcome the nucleosomal barrier for efficient RNA s…
View article: Specialization of RNA Polymerase I in Comparison to Other Nuclear RNA Polymerases of Saccharomyces cerevisiae
Specialization of RNA Polymerase I in Comparison to Other Nuclear RNA Polymerases of Saccharomyces cerevisiae Open
In archaea and bacteria the major classes of RNAs are synthesized by one DNA-dependent RNA polymerase (RNAP). In contrast, most eukaryotes have three highly specialized RNAPs to transcribe the nuclear genome. RNAP I synthesizes almost excl…
View article: The C-terminal region of Net1 is an activator of RNA polymerase I transcription with conserved features from yeast to human
The C-terminal region of Net1 is an activator of RNA polymerase I transcription with conserved features from yeast to human Open
RNA polymerase I (Pol I) synthesizes ribosomal RNA (rRNA) in all eukaryotes, accounting for the major part of transcriptional activity in proliferating cells. Although basal Pol I transcription factors have been characterized in diverse or…
View article: Toehold-enhanced LNA probes for selective pull down and single-molecule analysis of native chromatin
Toehold-enhanced LNA probes for selective pull down and single-molecule analysis of native chromatin Open
The organization of DNA into chromatin is thought to regulate gene expression in eukaryotes. To study its structure in vitro , there is a need for techniques that can isolate specific chromosomal loci of natively assembled chromatin. Curre…