Chuan‐Chih Hsu
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View article: Integrated Single-Tip IMAC-HILIC Enables Simultaneous Analysis of Plant Phosphoproteomics and N-Glycoproteomics
Integrated Single-Tip IMAC-HILIC Enables Simultaneous Analysis of Plant Phosphoproteomics and N-Glycoproteomics Open
Protein phosphorylation and N-glycosylation are key post-translational modifications (PTMs) in plants that regulate critical signaling processes. However, coanalysis of these PTMs is often complicated by their relatively low abundance and …
View article: Spatial proteomics reveals lipid droplet reorganization in symbiotic <i>Paramecium bursaria</i> cells
Spatial proteomics reveals lipid droplet reorganization in symbiotic <i>Paramecium bursaria</i> cells Open
Endosymbiosis is an important adaptive mechanism allowing organisms to exploit novel niches. The flexible relationship between the ciliate Paramecium bursaria and green algae represents a model system for studying early endosymbiosis evolu…
View article: Stabilization of HSA32, an aggregation-prone protein, by the protein disaggregase HSP101 plays a critical role in maintaining acquired thermotolerance in Arabidopsis
Stabilization of HSA32, an aggregation-prone protein, by the protein disaggregase HSP101 plays a critical role in maintaining acquired thermotolerance in Arabidopsis Open
Heat acclimation confers acquired thermotolerance (AT), and heat-acclimation memory (HAM) is the maintenance of AT for some time. In Arabidopsis and rice, the heat-stress-associated 32-kDa protein (HSA32) and the protein disaggregase heat …
View article: The nonphototrophic hypocotyl 3 (NPH3) domain protein NRL5 is a trafficking-associated GTPase essential for drought resistance
The nonphototrophic hypocotyl 3 (NPH3) domain protein NRL5 is a trafficking-associated GTPase essential for drought resistance Open
The mechanisms of plant drought resistance are unclear but may involve membrane trafficking and metabolic reprogramming, including proline accumulation. Forward genetic screening using a proline dehydrogenase 1 ( ProDH1 ) promoter:reporter…
View article: DIA-Based Phosphoproteomics Identifies Early Phosphorylation Events in Response to EGTA and Mannitol in Arabidopsis
DIA-Based Phosphoproteomics Identifies Early Phosphorylation Events in Response to EGTA and Mannitol in Arabidopsis Open
Osmotic stress significantly hampers plant growth and crop yields, emphasizing the need for a thorough comprehension of the underlying molecular responses. Previous research has demonstrated that osmotic stress rapidly induces calcium infl…
View article: A Sap Peptide Conserved across Flowering Plants Positively Regulates Lignin Biosynthesis, Biomass and Immunity
A Sap Peptide Conserved across Flowering Plants Positively Regulates Lignin Biosynthesis, Biomass and Immunity Open
Signaling peptides act as hormones to deliver short- or long-distance intercellular signals to govern complex developmental processes. Identifying endogenous signaling peptides is challenging due to their low abundance and the unknown clea…
View article: TIMAHAC: Streamlined Tandem IMAC-HILIC Workflow for Simultaneous and High-Throughput Plant Phosphoproteomics and N-glycoproteomics
TIMAHAC: Streamlined Tandem IMAC-HILIC Workflow for Simultaneous and High-Throughput Plant Phosphoproteomics and N-glycoproteomics Open
View article: TIMAHAC: Streamlined Tandem IMAC-HILIC Workflow for Simultaneous and High-Throughput Plant Phosphoproteomics and N-glycoproteomics
TIMAHAC: Streamlined Tandem IMAC-HILIC Workflow for Simultaneous and High-Throughput Plant Phosphoproteomics and N-glycoproteomics Open
Protein post-translational modifications (PTMs) are crucial in plant cellular processes, particularly in protein folding and signal transduction. N-glycosylation and phosphorylation are notably significant PTMs, playing essential roles in …
View article: A Tip-Based Workflow for Sensitive IMAC-Based Low Nanogram Level Phosphoproteomics
A Tip-Based Workflow for Sensitive IMAC-Based Low Nanogram Level Phosphoproteomics Open
View article: Ustilago maydis PR-1-like protein has evolved two distinct domains for dual virulence activities
Ustilago maydis PR-1-like protein has evolved two distinct domains for dual virulence activities Open
View article: The EXO70 inhibitor Endosidin2 alters plasma membrane protein composition in Arabidopsis roots
The EXO70 inhibitor Endosidin2 alters plasma membrane protein composition in Arabidopsis roots Open
To sustain normal growth and allow rapid responses to environmental cues, plants alter the plasma membrane protein composition under different conditions presumably by regulation of delivery, stability, and internalization. Exocytosis is a…
View article: The Non-Phototrophic Hypocotyl3 (NPH3)-domain protein NRL5 is a trafficking-associated GTPase essential for drought resistance
The Non-Phototrophic Hypocotyl3 (NPH3)-domain protein NRL5 is a trafficking-associated GTPase essential for drought resistance Open
The mechanisms of plant resistance to low water potential (ψ w ) during drought are unclear but may involve signaling and trafficking at the plasma membrane as well as metabolic reprogramming, including proline accumulation. Forward geneti…
View article: Supplementary Table 2 from EZH2 Modifies Sunitinib Resistance in Renal Cell Carcinoma by Kinome Reprogramming
Supplementary Table 2 from EZH2 Modifies Sunitinib Resistance in Renal Cell Carcinoma by Kinome Reprogramming Open
Gene list for Fig.5B, C, D
View article: Supplementary Table 2 from EZH2 Modifies Sunitinib Resistance in Renal Cell Carcinoma by Kinome Reprogramming
Supplementary Table 2 from EZH2 Modifies Sunitinib Resistance in Renal Cell Carcinoma by Kinome Reprogramming Open
Gene list for Fig.5B, C, D
View article: Data from EZH2 Modifies Sunitinib Resistance in Renal Cell Carcinoma by Kinome Reprogramming
Data from EZH2 Modifies Sunitinib Resistance in Renal Cell Carcinoma by Kinome Reprogramming Open
Acquired and intrinsic resistance to receptor tyrosine kinase inhibitors (RTKi) represents a major hurdle in improving the management of clear cell renal cell carcinoma (ccRCC). Recent reports suggest that drug resistance is driven by tumo…
View article: Supplemental Figures from EZH2 Modifies Sunitinib Resistance in Renal Cell Carcinoma by Kinome Reprogramming
Supplemental Figures from EZH2 Modifies Sunitinib Resistance in Renal Cell Carcinoma by Kinome Reprogramming Open
Supplemental data on additional models to support the role of EZH2 in RTKi resistance
View article: Data from EZH2 Modifies Sunitinib Resistance in Renal Cell Carcinoma by Kinome Reprogramming
Data from EZH2 Modifies Sunitinib Resistance in Renal Cell Carcinoma by Kinome Reprogramming Open
Acquired and intrinsic resistance to receptor tyrosine kinase inhibitors (RTKi) represents a major hurdle in improving the management of clear cell renal cell carcinoma (ccRCC). Recent reports suggest that drug resistance is driven by tumo…
View article: Supplemental Figures from EZH2 Modifies Sunitinib Resistance in Renal Cell Carcinoma by Kinome Reprogramming
Supplemental Figures from EZH2 Modifies Sunitinib Resistance in Renal Cell Carcinoma by Kinome Reprogramming Open
Supplemental data on additional models to support the role of EZH2 in RTKi resistance
View article: Supplementary Figure Legend from EZH2 Modifies Sunitinib Resistance in Renal Cell Carcinoma by Kinome Reprogramming
Supplementary Figure Legend from EZH2 Modifies Sunitinib Resistance in Renal Cell Carcinoma by Kinome Reprogramming Open
Supplementary Figure Legend
View article: Supplementary Figure Legend from EZH2 Modifies Sunitinib Resistance in Renal Cell Carcinoma by Kinome Reprogramming
Supplementary Figure Legend from EZH2 Modifies Sunitinib Resistance in Renal Cell Carcinoma by Kinome Reprogramming Open
Supplementary Figure Legend
View article: A Suspension Trapping–Based Sample Preparation Workflow for Sensitive Plant Phosphoproteomics
A Suspension Trapping–Based Sample Preparation Workflow for Sensitive Plant Phosphoproteomics Open
Plant phosphoproteomics provides a global view of phosphorylation-mediated signaling in plants; however, it demands high-throughput methods with sensitive detection and accurate quantification. Although protein precipitation is indispensab…
View article: SPINDLY mediates <i>O</i>-fucosylation of hundreds of proteins and sugar-dependent growth in Arabidopsis
SPINDLY mediates <i>O</i>-fucosylation of hundreds of proteins and sugar-dependent growth in Arabidopsis Open
The recent discovery of SPINDLY (SPY)-catalyzed protein O-fucosylation revealed a novel mechanism for regulating nucleocytoplasmic protein functions in plants. Genetic evidence indicates the important roles of SPY in diverse developmental …
View article: Mapping the signaling network of BIN2 kinase using TurboID-mediated biotin labeling and phosphoproteomics
Mapping the signaling network of BIN2 kinase using TurboID-mediated biotin labeling and phosphoproteomics Open
Elucidating enzyme–substrate relationships in posttranslational modification (PTM) networks is crucial for understanding signal transduction pathways but is technically difficult because enzyme–substrate interactions tend to be transient. …
View article: A streamlined tandem tip-based workflow for sensitive nanoscale phosphoproteomics
A streamlined tandem tip-based workflow for sensitive nanoscale phosphoproteomics Open
View article: Ustilago maydis PR-1-like protein has evolved two distinct domains for dual virulence activities
Ustilago maydis PR-1-like protein has evolved two distinct domains for dual virulence activities Open
Diversification of effector function, driven by a co-evolutionary arms race, enables pathogens to establish compatible interactions with their hosts. Structurally conserved plant pathogenesis-related PR-1 and PR-1-like (PR-1L) proteins are…
View article: An Integrated Proteomic Strategy to Identify SHP2 Substrates
An Integrated Proteomic Strategy to Identify SHP2 Substrates Open
Protein phosphatases play an essential role in normal cell physiology and the development of diseases such as cancer. The innate challenges associated with studying protein phosphatases have limited our understanding of their substrates, m…
View article: A streamlined tandem tip-based workflow for sensitive nanoscale phosphoproteomics
A streamlined tandem tip-based workflow for sensitive nanoscale phosphoproteomics Open
Effective phosphoproteome of nanoscale sample analysis remains a daunting task, primarily due to significant sample loss associated with non-specific surface adsorption during enrichment of low stoichiometric phosphopeptide. We developed a…
View article: Profiling of associated proteins reveals dual functions of the phosphatase ABI1 in abscisic acid signaling
Profiling of associated proteins reveals dual functions of the phosphatase ABI1 in abscisic acid signaling Open
Clade A PP2C phosphatases are central components of the ABA-receptor coupled core signaling pathway, and are involved in multiple stress responses and developmental processes. However, the direct targets or partner proteins of A clade PP2C…
View article: A cell type-specific multiomics uncovers a guard cell-specific RAF15-SnRK2.6/OST1 kinase cascade
A cell type-specific multiomics uncovers a guard cell-specific RAF15-SnRK2.6/OST1 kinase cascade Open
SUMMARY Multicellular organisms such as plants contain different cell types with specialized functions. Analyzing the characteristics of each cell type reveals specific cell functions and enhances understanding of organization and function…
View article: Corrigendum to: Plant U-Box 40 mediates degradation of the brassinosteroid-responsive transcription factor BZR1 in Arabidopsis roots
Corrigendum to: Plant U-Box 40 mediates degradation of the brassinosteroid-responsive transcription factor BZR1 in Arabidopsis roots Open