Chuanfeng Wu
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View article: Lineage bias in hematopoietic stem cells: more niche or intrinsic factors?
Lineage bias in hematopoietic stem cells: more niche or intrinsic factors? Open
Not available.
View article: Optimization of lentiviral delivery of barcoded anti-CD20 chimeric antigen receptors into rhesus macaque and human natural killer cells
Optimization of lentiviral delivery of barcoded anti-CD20 chimeric antigen receptors into rhesus macaque and human natural killer cells Open
View article: Long‐term tracking of haematopoietic clonal dynamics and mutations in non‐human primate undergoing transplantation of lentivirally barcoded haematopoietic stem and progenitor cells
Long‐term tracking of haematopoietic clonal dynamics and mutations in non‐human primate undergoing transplantation of lentivirally barcoded haematopoietic stem and progenitor cells Open
Summary Haematopoietic stem and progenitor cell (HSPC) autologous gene therapies are promising treatment for a variety of blood disorders. Investigation of the long‐term HSPC clonal dynamics and other measures of safety and durability foll…
View article: 266 Efficient lentiviral transduction of genetically barcoded AntiCD20-CAR-IL15 cassette into peripheral blood-derived rhesus macaque NK cells using poloxamer 407 and CAR-NK<i>in vitro</i>and<i>in vivo</i>effects
266 Efficient lentiviral transduction of genetically barcoded AntiCD20-CAR-IL15 cassette into peripheral blood-derived rhesus macaque NK cells using poloxamer 407 and CAR-NK<i>in vitro</i>and<i>in vivo</i>effects Open
View article: Impact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques
Impact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques Open
View article: Impact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques
Impact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques Open
View article: Impact of CRISPR/HDR-editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques
Impact of CRISPR/HDR-editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques Open
For precise genome editing via CRISPR/homology-directed repair (HDR), effective and safe editing of long-term engrafting hematopoietic stem cells (LT-HSCs) requires both sufficient HDR efficiency and protection of LT-HSC function and numbe…
View article: Ongoing production of tissue-resident macrophages from hematopoietic stem cells in healthy adult macaques
Ongoing production of tissue-resident macrophages from hematopoietic stem cells in healthy adult macaques Open
Macrophages orchestrate tissue immunity from the initiation and resolution of antimicrobial immune responses to the repair of damaged tissue. Murine studies demonstrate that tissue-resident macrophages are a heterogenous mixture of yolk sa…
View article: Improving the registration stability of cone-beam computed tomography with the Sphere-Mask Optical Positioning System: a feasibility study
Improving the registration stability of cone-beam computed tomography with the Sphere-Mask Optical Positioning System: a feasibility study Open
This study shows that S-M_OPS registration offers comparable accuracy to CBCT for daily registration. S-M_OPS, as an independent third-party tool, can prevent large errors in CBCT registration, thereby improving the accuracy and stability …
View article: Expanded NK cells used for adoptive cell therapy maintain diverse clonality and contain long-lived memory-like NK cell populations
Expanded NK cells used for adoptive cell therapy maintain diverse clonality and contain long-lived memory-like NK cell populations Open
View article: Ongoing Production of Tissue-Resident Macrophages from Hematopoietic Stem Cells in Healthy Adult Macaques
Ongoing Production of Tissue-Resident Macrophages from Hematopoietic Stem Cells in Healthy Adult Macaques Open
Macrophages are critical orchestrators of tissue immunity, from the initiation and resolution of antimicrobial immune responses to the subsequent repair of damaged tissue. Murine studies have demonstrated that tissue-resident macrophages a…
View article: Comparison of busulfan and total body irradiation conditioning on hematopoietic clonal dynamics following lentiviral gene transfer in rhesus macaques
Comparison of busulfan and total body irradiation conditioning on hematopoietic clonal dynamics following lentiviral gene transfer in rhesus macaques Open
View article: Barcode clonal tracking of tissue-resident immune cells in rhesus macaque highlights distinct clonal distribution pattern of tissue NK cells
Barcode clonal tracking of tissue-resident immune cells in rhesus macaque highlights distinct clonal distribution pattern of tissue NK cells Open
Tissue resident (TR) immune cells play important roles in facilitating tissue homeostasis, coordinating immune responses against infections and tumors, and maintaining immunological memory. While studies have shown these cells are distinct…
View article: Setup error assessment based on “Sphere-Mask” Optical Positioning System: Results from a multicenter study
Setup error assessment based on “Sphere-Mask” Optical Positioning System: Results from a multicenter study Open
Background The setup accuracy plays an extremely important role in the local control of tumors. The purpose of this study is to verify the feasibility of "Sphere-Mask" Optical Positioning System (S-M_OPS) for fast and accurate setup. Metho…
View article: Tissue Trafficking Kinetics of Rhesus Macaque Natural Killer Cells Measured by Serial Intravascular Staining
Tissue Trafficking Kinetics of Rhesus Macaque Natural Killer Cells Measured by Serial Intravascular Staining Open
The in vivo tissue distribution and trafficking patterns of natural killer (NK) cells remain understudied. Animal models can help bridge the gap, and rhesus macaque (RM) primates faithfully recapitulate key elements of human NK cell biolog…
View article: Interrogation of clonal tracking data using barcodetrackR
Interrogation of clonal tracking data using barcodetrackR Open
View article: Comparative engraftment and clonality of macaque HSPCs expanded on human umbilical vein endothelial cells versus non-expanded cells
Comparative engraftment and clonality of macaque HSPCs expanded on human umbilical vein endothelial cells versus non-expanded cells Open
View article: Clonal tracking of haematopoietic cells: insights and clinical implications
Clonal tracking of haematopoietic cells: insights and clinical implications Open
Summary Recent advances in high‐throughput genomics have enabled the direct tracking of outputs from many cell types, greatly accelerating the study of developmental processes and tissue regeneration. The capacity for long‐term self‐renewa…
View article: <i>barcodetrackR</i>: an R package for the interrogation of clonal tracking data
<i>barcodetrackR</i>: an R package for the interrogation of clonal tracking data Open
Clonal tracking methods provide quantitative insights into the cellular output of genetically labelled progenitor cells across time and cellular compartments. In the context of gene and cell therapies, clonal tracking methods have enabled …
View article: Impact of CMV Infection on Natural Killer Cell Clonal Repertoire in CMV-Naïve Rhesus Macaques
Impact of CMV Infection on Natural Killer Cell Clonal Repertoire in CMV-Naïve Rhesus Macaques Open
Recent functional, gene expression, and epigenetic studies have suggested the presence of a subset of mature natural killer (NK) cells responsible for maintaining NK cell memory. The lack of endogenous clonal markers in NK cells impedes un…
View article: Clonal tracking of erythropoiesis in rhesus macaques
Clonal tracking of erythropoiesis in rhesus macaques Open
The classical model of hematopoietic hierarchies is being reconsidered on the basis of data from in vitro assays and single cell expression profiling. Recent experiments suggested that the erythroid lineage might differentiate direc…
View article: Aberrant Clonal Hematopoiesis following Lentiviral Vector Transduction of HSPCs in a Rhesus Macaque
Aberrant Clonal Hematopoiesis following Lentiviral Vector Transduction of HSPCs in a Rhesus Macaque Open
View article: Aberrant Clonal Hematopoiesis Following Lentiviral Transduction of HSPC in a Rhesus Macaque
Aberrant Clonal Hematopoiesis Following Lentiviral Transduction of HSPC in a Rhesus Macaque Open
Lentiviral vectors (LV) have been used for the delivery of genes into hematopoietic stem and progenitor cells (HSPC) in clinical trials worldwide. LV, in contrast to retroviral vectors, have not been associated with insertion site-associat…
View article: Clonal expansion and compartmentalized maintenance of rhesus macaque NK cell subsets
Clonal expansion and compartmentalized maintenance of rhesus macaque NK cell subsets Open
Macaque barcoding reveals NK oligoclonal expansion and persistence independent of production from HSPCs or immature NK cells.
View article: Barcoding of Macaque Hematopoietic Stem and Progenitor Cells: A Robust Platform to Assess Vector Genotoxicity
Barcoding of Macaque Hematopoietic Stem and Progenitor Cells: A Robust Platform to Assess Vector Genotoxicity Open
Gene therapies using integrating retrovirus vectors to modify hematopoietic stem and progenitor cells have shown great promise for the treatment of immune system and hematologic diseases. However, activation of proto-oncogenes via insertio…
View article: Physically-Based Modeling and Characterization of Hot Flow Behavior in an Interphase-Precipitated Ti-Mo Microalloyed Steel
Physically-Based Modeling and Characterization of Hot Flow Behavior in an Interphase-Precipitated Ti-Mo Microalloyed Steel Open
In this contribution, a series of hot compression tests was conducted on a typical interphase-precipitated Ti-Mo steel at relatively higher strain rates of 0.1~10 s−1 and temperatures of 900~1150 °C using a Gleeble-2000 thermo-mechanical s…
View article: Faculty Opinions recommendation of Restoration of TET2 Function Blocks Aberrant Self-Renewal and Leukemia Progression.
Faculty Opinions recommendation of Restoration of TET2 Function Blocks Aberrant Self-Renewal and Leukemia Progression. Open
View article: The impact of aging on primate hematopoiesis as interrogated by clonal tracking
The impact of aging on primate hematopoiesis as interrogated by clonal tracking Open
Key Points Genetic barcoding of HSPCs in aged macaques reveals impaired long-term clonal output from multipotent HSPCs. Aged macaques showed prolonged contributions from lineage-biased HSPCs and late clonal expansions.
View article: Geographic clonal tracking in macaques provides insights into HSPC migration and differentiation
Geographic clonal tracking in macaques provides insights into HSPC migration and differentiation Open
The geographic distribution of hematopoiesis at a clonal level is of interest in understanding how hematopoietic stem and progenitor cells (HSPCs) and their progeny interact with bone marrow (BM) niches during regeneration. We tagged rhesu…
View article: Quantitative stability of hematopoietic stem and progenitor cell clonal output in rhesus macaques receiving transplants
Quantitative stability of hematopoietic stem and progenitor cell clonal output in rhesus macaques receiving transplants Open
Key Points Output from individual rhesus macaque hematopoietic stem and progenitor cells is stable for years, with little evidence of clonal succession. Individual clones may display stable myeloid or lymphoid bias for many years.