Chufeng Li
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View article: Cascade‐Targeted Nanoparticles for Enhanced Gemcitabine Delivery and Adenosine Metabolism Modulation to Overcome Treatment Resistance in Pancreatic Cancer
Cascade‐Targeted Nanoparticles for Enhanced Gemcitabine Delivery and Adenosine Metabolism Modulation to Overcome Treatment Resistance in Pancreatic Cancer Open
KRAS mutations are find in over 90% of pancreatic ductal adenocarcinoma (PDAC) cases, making PDAC exhibit intrinsic resistance to chemotherapy and reshape the immunosuppressive tumor microenvironment (TME), disappointing the clinically pre…
View article: Focusing of X-ray free-electron laser pulses using multilayer Laue lenses
Focusing of X-ray free-electron laser pulses using multilayer Laue lenses Open
Achieving the highest possible intensities of pulses at X-ray free-electron laser (XFEL) facilities entails focusing the X-ray beam to the smallest possible dimensions. Multilayer Laue lenses are volume diffractive optics that hold promise…
View article: A Methionine Allocation Nanoregulator for the Suppression of Cancer Stem Cells and Support to the Immune Cells by Epigenetic Regulation
A Methionine Allocation Nanoregulator for the Suppression of Cancer Stem Cells and Support to the Immune Cells by Epigenetic Regulation Open
Epigenetic dysregulation is prevalent in human cancers, affecting gene expression and metabolic patterns to meet the demands of malignant evolution and abnormal epigenetic processes, and resulting in a protumor immune microenvironment. Tum…
View article: Convergent-beam attosecond x-ray crystallography
Convergent-beam attosecond x-ray crystallography Open
Sub-ångström spatial resolution of electron density coupled with sub-femtosecond to few-femtosecond temporal resolution is required to directly observe the dynamics of the electronic structure of a molecule after photoinitiation or some ot…
View article: Convergent-beam attosecond X-ray crystallography
Convergent-beam attosecond X-ray crystallography Open
Sub-angstrom spatial resolution of electron density coupled with sub-femtosecond temporal resolution is required to directly observe the dynamics of the electronic structure of a molecule after photoinitiation or some other ultrafast pertu…
View article: Fast and efficient hard X-ray projection imaging below 10 nm resolution
Fast and efficient hard X-ray projection imaging below 10 nm resolution Open
High-resolution X-ray imaging of noncrystalline objects is often achieved through the approach of scanning coherent diffractive imaging known as ptychography. The imaging resolution is usually limited by the scattering properties of the sa…
View article: X-ray focusing below 3 nm with aberration-corrected multilayer Laue lenses
X-ray focusing below 3 nm with aberration-corrected multilayer Laue lenses Open
Multilayer Laue lenses are volume diffractive optical elements for hard X-rays with the potential to focus beams to sizes as small as 1 nm. This ability is limited by the precision of the manufacturing process, whereby systematic errors th…
View article: Hard X-ray projection imaging below 5 nm resolution
Hard X-ray projection imaging below 5 nm resolution Open
High-resolution X-ray imaging of non-crystalline objects is often achieved through the approach of scanning coherent diffractive imaging known as ptychography. The imaging resolution is usually limited by the scattering properties of the s…
View article: An End-to-End Structure with Novel Position Mechanism and Improved EMD for Stock Forecasting
An End-to-End Structure with Novel Position Mechanism and Improved EMD for Stock Forecasting Open
As a branch of time series forecasting, stock movement forecasting is one of the challenging problems for investors and researchers. Since Transformer was introduced to analyze financial data, many researchers have dedicated themselves to …
View article: Nanomaterials with dual immunomodulatory functions for synergistic therapy of breast cancer brain metastases
Nanomaterials with dual immunomodulatory functions for synergistic therapy of breast cancer brain metastases Open
A long-standing paucity of effective therapies results in the poor outcomes of triple-negative breast cancer brain metastases. Immunotherapy has made progress in the treatment of tumors, but limited by the non-immunogenicity of tumors and …
View article: Imaging via Correlation of X-Ray Fluorescence Photons
Imaging via Correlation of X-Ray Fluorescence Photons Open
We demonstrate that x-ray fluorescence emission, which cannot maintain a stationary interference pattern, can be used to obtain images of structures by recording photon-photon correlations in the manner of the stellar intensity interferome…
View article: Convergent beam X-ray diffraction using multilayer Laue lenses – an exploration following in John's footsteps
Convergent beam X-ray diffraction using multilayer Laue lenses – an exploration following in John's footsteps Open
View article: Robust ptychographic X-ray speckle tracking with multilayer Laue lenses
Robust ptychographic X-ray speckle tracking with multilayer Laue lenses Open
In recent years, X-ray speckle tracking techniques have emerged as viable tools for wavefront metrology and sample imaging applications, and have been actively developed for use at synchrotron light sources. Speckle techniques can recover …
View article: On the use of multilayer Laue lenses with X-ray Free Electron Lasers
On the use of multilayer Laue lenses with X-ray Free Electron Lasers Open
Multilayer Laue lenses were used for the first time to focus x-rays from an X-ray Free Electron Laser (XFEL). In an experiment, which was performed at the European XFEL, we demonstrated focusing to a spot size of a few tens of nanometers. …
View article: The Ratio of NT-proBNP to CysC1.53 Predicts Heart Failure in Patients With Chronic Kidney Disease
The Ratio of NT-proBNP to CysC1.53 Predicts Heart Failure in Patients With Chronic Kidney Disease Open
Background: The N-terminal pro B type natriuretic peptide (NT-proBNP) is important for prognosis of heart failure in patients with chronic kidney disease (CKD). However, the NT-proBNP level is easily affected by renal insufficiency, which …
View article: Macrophage‐Disguised Manganese Dioxide Nanoparticles for Neuroprotection by Reducing Oxidative Stress and Modulating Inflammatory Microenvironment in Acute Ischemic Stroke
Macrophage‐Disguised Manganese Dioxide Nanoparticles for Neuroprotection by Reducing Oxidative Stress and Modulating Inflammatory Microenvironment in Acute Ischemic Stroke Open
Reperfusion injury is still a major challenge that impedes neuronal survival in ischemic stroke. However, the current clinical treatments are remained on single pathological process, which are due to lack of comprehensive neuroprotective e…
View article: On the use of multilayer Laue lenses with X-ray free electron lasers
On the use of multilayer Laue lenses with X-ray free electron lasers Open
We report on the use of multilayer Laue lenses to focus the intense X-ray Free Electron Laser (XFEL) beam at the European XFEL to a spot size of a few tens of nanometers. We present the procedure to align and characterize these lenses and …
View article: X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease
X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease Open
A large-scale screen to target SARS-CoV-2 The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome is initially expressed as two large polyproteins. Its main protease, M pro , is essential to yield functional viral proteins,…
View article: Early-stage dynamics of chloride ion–pumping rhodopsin revealed by a femtosecond X-ray laser
Early-stage dynamics of chloride ion–pumping rhodopsin revealed by a femtosecond X-ray laser Open
Significance Light-driven rhodopsin proteins pump ions across cell membranes. They have applications in optogenetics and can potentially be used to develop solar energy–harvesting devices. A detailed understanding of rhodopsin dynamics and…
View article: Synchronous RNA conformational changes trigger ordered phase transitions in crystals
Synchronous RNA conformational changes trigger ordered phase transitions in crystals Open
View article: Ultrafast structural changes within a photosynthetic reaction centre
Ultrafast structural changes within a photosynthetic reaction centre Open
Photosynthetic reaction centres harvest the energy content of sunlight by transporting electrons across an energy-transducing biological membrane. Here we use time-resolved serial femtosecond crystallography1 using an X-ray free-electron l…
View article: Ultrafast structural changes within a photosynthetic reaction centre
Ultrafast structural changes within a photosynthetic reaction centre Open
View article: Inhibition of SARS-CoV-2 main protease by allosteric drug-binding
Inhibition of SARS-CoV-2 main protease by allosteric drug-binding Open
The coronavirus disease (COVID-19) caused by SARS-CoV-2 is creating tremendous health problems and economical challenges for mankind. To date, no effective drug is available to directly treat the disease and prevent virus spreading. In a s…
View article: Enhanced X-ray diffraction of <i>in vivo</i>-grown μNS crystals by viscous jets at XFELs
Enhanced X-ray diffraction of <i>in vivo</i>-grown μNS crystals by viscous jets at XFELs Open
μNS is a 70 kDa major nonstructural protein of avian reoviruses, which cause significant economic losses in the poultry industry. They replicate inside viral factories in host cells, and the μNS protein has been suggested to be the minimal…
View article: Catalytic cleavage of HEAT and subsequent covalent binding of the tetralone moiety by the SARS-CoV-2 main protease
Catalytic cleavage of HEAT and subsequent covalent binding of the tetralone moiety by the SARS-CoV-2 main protease Open
Here we present the crystal structure of SARS-CoV-2 main protease (M pro ) covalently bound to 2-methyl-1-tetralone. This complex was obtained by co-crystallization of M pro with HEAT (2-(((4-hydroxyphenethyl)amino)methyl)-3,4-dihydronapht…
View article: <i>SPIND-TC</i>: an indexing method for two-color X-ray diffraction data
<i>SPIND-TC</i>: an indexing method for two-color X-ray diffraction data Open
Recent developments of two-color operation modes at X-ray free-electron laser facilities provide new research opportunities, such as X-ray pump/X-ray probe experiments and multiple-wavelength anomalous dispersion phasing methods. However, …
View article: XFEL and NMR Structures of Francisella Lipoprotein Reveal Conformational Space of Drug Target against Tularemia
XFEL and NMR Structures of Francisella Lipoprotein Reveal Conformational Space of Drug Target against Tularemia Open
View article: Toward G protein-coupled receptor structure-based drug design using X-ray lasers
Toward G protein-coupled receptor structure-based drug design using X-ray lasers Open
Rational structure-based drug design (SBDD) relies on the availability of a large number of co-crystal structures to map the ligand-binding pocket of the target protein and use this information for lead-compound optimization via an iterati…
View article: The complementarity of serial femtosecond crystallography and MicroED for structure determination from microcrystals
The complementarity of serial femtosecond crystallography and MicroED for structure determination from microcrystals Open
View article: Structure and dynamics of chloride ion pumping rhodopsin revealed by time-resolved SFX and atomic molecular dynamics simulations
Structure and dynamics of chloride ion pumping rhodopsin revealed by time-resolved SFX and atomic molecular dynamics simulations Open