Claire M. Brown
YOU?
Author Swipe
View article: A guide to selecting high-performing antibodies for Heterogeneous nuclear ribonucleoproteins A2/B1(hnRNP A2/B1) (UniProt ID: P22626) for use in western blot, immunoprecipitation, and immunofluorescence
A guide to selecting high-performing antibodies for Heterogeneous nuclear ribonucleoproteins A2/B1(hnRNP A2/B1) (UniProt ID: P22626) for use in western blot, immunoprecipitation, and immunofluorescence Open
Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) is a key RNA-binding protein involved in alternative splicing, mRNA transport, and local translation processes essential for neuronal development and synaptic plasticity. Here we …
View article: Strengthening Research Through Scientific Platforms: Highlights from the 5th Canadian Network of Scientific Platforms (CNSP) Conference
Strengthening Research Through Scientific Platforms: Highlights from the 5th Canadian Network of Scientific Platforms (CNSP) Conference Open
View article: A Cloud-Based National Resource for FAIR Microscopy Research Data Management, Analysis and Sharing in Canada
A Cloud-Based National Resource for FAIR Microscopy Research Data Management, Analysis and Sharing in Canada Open
View article: Building momentum through networks: Bioimaging across the Americas
Building momentum through networks: Bioimaging across the Americas Open
In September 2023, the two largest bioimaging networks in the Americas, Latin America Bioimaging (LABI) and BioImaging North America (BINA), came together during a 1-week meeting in Mexico. This meeting provided opportunities for participa…
View article: Building momentum through networks: Bioimaging across the Americas
Building momentum through networks: Bioimaging across the Americas Open
In September 2023, the two largest bioimaging networks in the Americas, Latin America Bioimaging (LABI) and BioImaging North America (BINA), came together during a 1‐week meeting in Mexico. This meeting provided opportunities for participa…
View article: Recognising the importance and impact of Imaging Scientists: Global guidelines for establishing career paths within core facilities
Recognising the importance and impact of Imaging Scientists: Global guidelines for establishing career paths within core facilities Open
In the dynamic landscape of scientific research, imaging core facilities are vital hubs propelling collaboration and innovation at the technology development and dissemination frontier. Here, we present a collaborative effort led by Global…
View article: Recognising the importance and impact of Imaging Scientists: Global guidelines for establishing career paths within core facilities
Recognising the importance and impact of Imaging Scientists: Global guidelines for establishing career paths within core facilities Open
In the dynamic landscape of scientific research, imaging core facilities are vital hubs propelling collaboration and innovation at the technology development and dissemination frontier. Here, we present a collaborative effort led by Global…
View article: A consensus platform for antibody characterization
A consensus platform for antibody characterization Open
Antibody-based research applications are critical for biological discovery. Yet, there are no industry standards to compare the performance of antibodies in various applications. We describe a knockout cell line-based antibody characteriza…
View article: Harmonizing the Generation and Pre-publication Stewardship of FAIR Image Data
Harmonizing the Generation and Pre-publication Stewardship of FAIR Image Data Open
Alongside molecular insights into genes and proteins, biological imaging holds great promise for deepening scientific understanding of complex cellular systems and advancing predictive, personalized therapies for human health. To realize t…
View article: Author Response: Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
Author Response: Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications Open
Full text Figures and data Side by side Abstract eLife assessment eLife digest Introduction Results Discussion Materials and methods Data availability References Peer review Author response Article and author information Metrics Abstract A…
View article: Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications Open
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of the problem remains largely anecdotal,…
View article: Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications Open
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of the problem remains largely anecdotal,…
View article: Reviewer #2 (Public Review): Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
Reviewer #2 (Public Review): Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications Open
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of the problem remains largely anecdotal,…
View article: Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications Open
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of the problem remains largely anecdotal,…
View article: Author Response: Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
Author Response: Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications Open
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of the problem remains largely anecdotal,…
View article: Reviewer #1 (Public Review): Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
Reviewer #1 (Public Review): Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications Open
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of the problem remains largely anecdotal,…
View article: Antibody Characterization Report for Calponin-3
Antibody Characterization Report for Calponin-3 Open
<p>Head-to-head comparison of available commercial antibodies against Calponin-3 by immunoblot (Western blot), immunoprecipitation and immunofluorescence. Work reported in this study was supported in part by the Emory-Sage-…
View article: Antibody Characterization Report for Rab-1A and Rab-1B
Antibody Characterization Report for Rab-1A and Rab-1B Open
<p>Head-to-head comparison of available commercial antibodies against Rab1 proteins Rab-1A and Rab-1B by immunoblot (Western blot), immunoprecipitation and immunofluorescence.</p>
View article: Antibody Characterization Report for Ras-related protein Rab5A
Antibody Characterization Report for Ras-related protein Rab5A Open
<p>Head-to-head comparison of available commercial antibodies against Ras-related protein Rab5A by immunoblot (Western blot), immunoprecipitation and immunofluorescence.</p>
View article: How to Measure the Illumination Overhead (IO) of a Fluorescence Microscope v1
How to Measure the Illumination Overhead (IO) of a Fluorescence Microscope v1 Open
This protocol outlines in detail how to measure illumination overhead (IO) and collect raw illumination intensity data using the Rigol ds1054z oscilloscope. In fluorescence microscopy, IO is defined as the time a sample is illuminated with…
View article: Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications Open
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of the problem remains largely anecdotal,…
View article: Antibody Characterization Report for Leucine-rich repeat kinase 2 (LRRK2)
Antibody Characterization Report for Leucine-rich repeat kinase 2 (LRRK2) Open
<p>Head-to-head comparison of available commercial antibodies against Leucine-rich repeat kinase 2 (LRRK2) by immunoblot (Western blot), immunoprecipitation and immunofluorescence. Work reported in this report was supported in p…
View article: Antibody Characterization Report for Plasma membrane calcium-transporting ATPase 1
Antibody Characterization Report for Plasma membrane calcium-transporting ATPase 1 Open
Head-to-head comparison of available commercial antibodies against Plasma membrane calcium-transporting ATPase 1 by immunoblot (Western blot), immunoprecipitation and immunofluorescence. Work reported in this report was supported in part b…
View article: Antibody Characterization Report for Amyloid-beta precursor protein
Antibody Characterization Report for Amyloid-beta precursor protein Open
A peer-reviewed antibody characterization article corresponding to this Zenodo preprint is openly available at F1000Research: https://doi.org/10.12688/f1000research.139867.1 This report presents a guide to selecting hi…
View article: Antibody Characterization Report for Prolow-density lipoprotein receptor-related protein1 (LRP-1)
Antibody Characterization Report for Prolow-density lipoprotein receptor-related protein1 (LRP-1) Open
Head-to-head comparison of available commercial antibodies against Prolow-density lipoprotein receptor-related protein1 (LRP-1) by immunoblot (Western blot), immunoprecipitation and immunofluorescence. Work reported in this report was supp…
View article: Antibody Characterization Report for Valosin-containing protein VCP
Antibody Characterization Report for Valosin-containing protein VCP Open
Head-to-head comparison of available commercial antibodies against Valosin-containing protein VCP by immunoblot (Western blot), immunoprecipitation and immunofluorescence. Work reported in this report was supported in part by the ALS-Repro…
View article: Antibody Characterization Report for Leucine-rich repeat kinase 2 (LRRK2)
Antibody Characterization Report for Leucine-rich repeat kinase 2 (LRRK2) Open
Head-to-head comparison of available commercial antibodies against Leucine-rich repeat kinase 2 (LRRK2) by immunoblot (Western blot), immunoprecipitation and immunofluorescence. The following study was funded in part by Genome Québec's Ge…
View article: Polarity and mixed-mode oscillations may underlie different patterns of cellular migration
Polarity and mixed-mode oscillations may underlie different patterns of cellular migration Open
View article: Tissue-Like 3D Standard and Protocols for Microscope Quality Management
Tissue-Like 3D Standard and Protocols for Microscope Quality Management Open
This article outlines a global study conducted by the Association of Biomedical Resource Facilities (ABRF) Light Microscopy Research Group (LMRG). The results present a novel 3D tissue-like biologically relevant standard sample that is aff…
View article: Antibody Characterization Report for RNA-binding protein TIA1
Antibody Characterization Report for RNA-binding protein TIA1 Open
A peer-reviewed antibody characterization article corresponding to this Zenodo preprint is openly available at F1000Research: https://doi.org/10.12688/f1000research.133645.2 …