Clara Radulescu
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View article: Combining analysis of individual and wastewater whole genome sequencing improves SARS-CoV-2 surveillance
Combining analysis of individual and wastewater whole genome sequencing improves SARS-CoV-2 surveillance Open
Effective pathogen surveillance is critical for public health decision-making, with both individual and environmental monitoring playing essential roles. While wastewater (WW) and individual whole genome sequencing (WGS) have been used to …
View article: Histamine Produced by Gram-Negative Bacteria Impairs Neutrophil’s Antimicrobial Response by Engaging the Histamine 2 Receptor
Histamine Produced by Gram-Negative Bacteria Impairs Neutrophil’s Antimicrobial Response by Engaging the Histamine 2 Receptor Open
We found that histamine (10−9 M) did not have any effect on the in vitro capture of Escherichia coli by neutrophils but accelerated its intracellular killing. In contrast, histamine (10−6 M) delayed the …
View article: Mini-XT, a miniaturized tagmentation-based protocol for efficient sequencing of SARS-CoV-2
Mini-XT, a miniaturized tagmentation-based protocol for efficient sequencing of SARS-CoV-2 Open
Background The COVID-19 pandemic has highlighted the importance of whole genome sequencing (WGS) of SARS-CoV-2 to inform public health policy. By enabling definition of lineages it facilitates tracking of the global spread of the virus. Th…
View article: Additional file 1 of Mini-XT, a miniaturized tagmentation-based protocol for efficient sequencing of SARS-CoV-2
Additional file 1 of Mini-XT, a miniaturized tagmentation-based protocol for efficient sequencing of SARS-CoV-2 Open
Additional file 1. Reagents required for the Mini-XT protocol.
View article: Mini-XT, a miniaturized tagmentation-based protocol for efficient sequencing of SARS-CoV-2
Mini-XT, a miniaturized tagmentation-based protocol for efficient sequencing of SARS-CoV-2 Open
Introduction The COVID-19 pandemic has highlighted the importance of whole genome sequencing (WGS) of SARS-CoV-2 to inform public health policy. By enabling definition of lineages it facilitates tracking of the global spread of the virus. …
View article: MiniXT protocol v1
MiniXT protocol v1 Open
We present the ‘mini-XT’ miniaturized tagmentation-based library preparation protocol used for Illumina WGS of SARS-CoV-2 positive samples. Reverse transcription and amplification is based upon the nCoV-2019 sequencing protocol v3 (LoCost)…