Explanipedia

A biomimetic model composed of injectable 3D muscle-like tissue, stromal and immune cells for recapitulating the rapid immune signature predictive of mRNA vaccine immunogenicity Open

Marilena P. Etna, Claudia Fuoco, Martina Severa, Daniela Ricci, Alessandro Sinigaglia , et al. · 2025

Background System vaccinology identified an early innate signature associated with vaccine-mediated protection whose induction is likely to involve both immune and non-immune cells. Methods To dissect muscle and stromal cell contribution, …

<span>SPP1</span> macrophages across diseases: A call for reclassification? Open

Alessio Reggio, Claudia Fuoco, Rebecca Deodati, Alessandro Palma · 2025

SPP1+ macrophages, characterized by elevated expression of the osteopontin gene (secreted phosphoprotein 1, SPP1), have emerged as key players in various pathological contexts, including aging, chronic inflammatory diseases, and cancer. Wh…

3D Rotary Wet‐Spinning (RoWS) Biofabrication Directly Affects Proteomic Signature and Myogenic Maturation in Muscle Pericyte–Derived Human Myo‐Substitute Open

Alessio Reggio, Claudia Fuoco, Francesca De Paolis, Rebecca Deodati, Stefano Testa , et al. · 2025

Skeletal muscle tissue engineering (SMTE) has recently emerged to address major clinical challenges such as volumetric muscle loss (VML). Here, we report a rotary wet‐spinning (RoWS) biofabrication technique for producing human myo‐substit…

Exploiting Mass Spectrometry to Unlock the Mechanism of Nanoparticle-Induced Inflammasome Activation Open

Govind Gupta, Jasreen Kaur, Kunal Bhattacharya, Benedict J. Chambers, Arianna Gazzi , et al. · 2023

Nanoparticles (NPs) elicit sterile inflammation, but the underlying signaling pathways are poorly understood. Here, we report that human monocytes are particularly vulnerable to amorphous silica NPs, as evidenced by single-cell-based analy…

Long-term longitudinal study on swine VML model Open

Francesca De Paolis, Stefano Testa, Gabriele Guarnaccia, Alessio Reggio, Ersilia Fornetti , et al. · 2023

Combining rotary wet-spinning biofabrication and electro-mechanical stimulation for the in vitro production of functional myo-substitutes Open

Nehar Celikkin, Dario Presutti, Fabio Maiullari, Marina Volpi, Yurii Promovych , et al. · 2023

In this work, we present an innovative, high-throughput rotary wet-spinning biofabrication method for manufacturing cellularized constructs composed of highly-aligned hydrogel fibers. The platform is supported by an innovative microfluidic…

3D bioprinting directly affects proteomic signature and myogenic maturation in muscle pericytes-derived human myo-substitute Open

Alessio Reggio, Claudia Fuoco, Francesca De Paolis, Stefano Testa, Nehar Celikkin , et al. · 2023

Skeletal muscle tissue engineering (SMTE) has recently emerged to address major clinical challenges such as volumetric muscle loss. Here, we report a rotary wet-spinning (RoWS) biofabrication technique for producing human myo-substitutes w…

A 3D adipogenesis platform to study the fate of fibro/adipogenic progenitors in muscular dystrophies Open

Alessio Reggio, Francesca De Paolis, Salma Bousselmi, Felice Cicciarelli, Sergio Bernardini , et al. · 2023

In human dystrophies, progressive muscle wasting is exacerbated by ectopic deposition of fat and fibrous tissue originating from fibro/adipogenic progenitors (FAPs). In degenerating muscles, the ability of these cells to promote successful…

A novel extrusion-based 3D bioprinting system for skeletal muscle tissue engineering Open

Ersilia Fornetti, Francesca De Paolis, Claudia Fuoco, Sergio Bernardini, Sara M Giannitelli , et al. · 2023

Three-dimensional (3D) bioprinting is an emerging technology, which turned out to be an optimal tool for tissue engineering approaches. To date, different printing systems have been developed. Among them, the extrusion-based approach demon…

Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry Open

Lucia Lisa Petrilli, Claudia Fuoco, Alessandro Palma, Luca Pasquini, Giulia Pericoli , et al. · 2022

Paediatric-type diffuse high-grade gliomas (PDHGG) are aggressive tumors affecting children and young adults, with no effective treatment. These highly heterogeneous malignancies arise in different sites of the Central Nervous System (CNS)…

In vivo restoration of dystrophin expression in mdx mice using intra-muscular and intra-arterial injections of hydrogel microsphere carriers of exon skipping antisense oligonucleotides Open

Shani Cohen, Orit Bar‐Am, Claudia Fuoco, Galit Saar, Cesare Gargioli , et al. · 2022

Graphene nanoribbons are internalized by human primary immune cell subpopulations maintaining a safety profile: A high-dimensional pilot study by single-cell mass cytometry Open

Claudia Fuoco, Xiangfeng Luan, Laura Fusco, Federica Riccio, Giulio Giuliani , et al. · 2022

HGG-46. Inter and Intra-tumor Heterogeneity of Pediatric-type Diffuse High-Grade Glioma Revealed by High-Dimensional Single-Cell Proteomics Open

Lucia Lisa Petrilli, Claudia Fuoco, Luca Pasquini, Alessandro Palma, Giulia Pericoli , et al. · 2022

Paediatric-type diffuse high-grade gliomas (PDHGG) are aggressive brain tumors, affecting children and young adults, with no effective treatments. A main constraint to the development of effective treatment is associated with their highly …

Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue Open

Marco Rosina, Veronica Ceci, Riccardo Turchi, Chuan Li, Nicholas Borcherding , et al. · 2022

Dystrophic Muscle Affects Motoneuron Axon Outgrowth and NMJ Assembly Open

Ersilia Fornetti, Stefano Testa, Francesca De Paolis, Claudia Fuoco, Sergio Bernardini , et al. · 2022

The Neuromuscular Junction (NMJ) is a chemical synapse localized between the terminal branches of the spinal motor neurons and myofibers. In the past two decades coculture systems to generate the NMJ in culture are developed to address con…

Transcription Factor Activation Profiles (TFAP) identify compounds promoting differentiation of Acute Myeloid Leukemia cell lines Open

Federica Riccio, Elisa Micarelli, Riccardo Secci, Giulio Giuliani, Simone Vumbaca , et al. · 2022

Graphene oxide activates B cells with upregulation of granzyme B expression: evidence at the single-cell level for its immune-modulatory properties and anticancer activity Open

Marco Orecchioni, Laura Fusco, Raghvendra Mall, Valentina Bordoni, Claudia Fuoco , et al. · 2021

While both graphene oxide and amino functionalized graphene oxide exert strong pro-activating properties on B cells, the latter is also able to induce a B cell receptor signaling dysregulation, which triggers the production of granzyme B.

Characterization of the Skeletal Muscle Secretome Reveals a Role for Extracellular Vesicles and IL1α/IL1β in Restricting Fibro/Adipogenic Progenitor Adipogenesis Open

Simone Vumbaca, Giulio Giuliani, Valeria Fiorentini, Flavia Tortolici, Andrea Cerquone Perpetuini , et al. · 2021

Repeated mechanical stress causes injuries in the adult skeletal muscle that need to be repaired. Although muscle regeneration is a highly efficient process, it fails in some pathological conditions, compromising tissue functionality. This…

Transcription Factor Activation Profiles (TFAP) identify compounds promoting differentiation of Acute Myeloid Leukemia cell lines Open

Federica Riccio, Elisa Micarelli, Riccardo Secci, Giulio Giuliani, Simone Vumbaca , et al. · 2021

Repurposing of drugs for new therapeutic use has received considerable attention for its potential to reduce time and cost of drug development. Here we present a new strategy to identify chemicals that are likely to induce differentiation …

Lateral dimension and amino-functionalization on the balance to assess the single-cell toxicity of graphene on fifteen immune cell types Open

Laura Fusco, Marco Orecchioni, Giacomo Reina, Valentina Bordoni, Claudia Fuoco , et al. · 2021

The War after War: Volumetric Muscle Loss Incidence, Implication, Current Therapies and Emerging Reconstructive Strategies, a Comprehensive Review Open

Stefano Testa, Ersilia Fornetti, Claudia Fuoco, Carles Sánchez Riera, Francesco Rizzo , et al. · 2021

Volumetric muscle loss (VML) is the massive wasting of skeletal muscle tissue due to traumatic events or surgical ablation. This pathological condition exceeds the physiological healing process carried out by the muscle itself, which owns …

A Resource for the Network Representation of Cell Perturbations Caused by SARS-CoV-2 Infection Open

Livia Perfetto, Elisa Micarelli, Marta Iannuccelli, Prisca Lo Surdo, Giulio Giuliani , et al. · 2021

The coronavirus disease 2019 (COVID-19) pandemic has caused more than 2.3 million casualties worldwide and the lack of effective treatments is a major health concern. The development of targeted drugs is held back due to a limited understa…

Skeletal Muscle Subpopulation Rearrangements upon Rhabdomyosarcoma Development through Single-Cell Mass Cytometry Open

Lucia Lisa Petrilli, Federica Riccio, Giulio Giuliani, Alessandro Palma, Cesare Gargioli , et al. · 2021

The embryonal rhabdomyosarcoma (eRMS) is a soft tissue sarcoma commonly affecting the head and neck, the extremities and the genitourinary tract. To contribute to revealing the cell types that may originate this tumor, we exploited mass cy…

Biofabricating murine and human myo‐substitutes for rapid volumetric muscle loss restoration Open

Marco Costantini, Stefano Testa, Ersilia Fornetti, Claudia Fuoco, Carles Sánchez Riera , et al. · 2021

The importance of skeletal muscle tissue is undoubted being the controller of several vital functions including respiration and all voluntary locomotion activities. However, its regenerative capability is limited and significant tissue los…

SCA-1 micro-heterogeneity in the fate decision of dystrophic fibro/adipogenic progenitors Open

Giulio Giuliani, Simone Vumbaca, Claudia Fuoco, Cesare Gargioli, Ezio Giorda , et al. · 2021

Skeletal Muscle-Derived Human Mesenchymal Stem Cells: Influence of Different Culture Conditions on Proliferative and Myogenic Capabilities Open

Stefano Testa, Carles Sánchez Riera, Ersilia Fornetti, Federica Riccio, Claudia Fuoco , et al. · 2020

Skeletal muscle tissue is characterized by restrained self-regenerative capabilities, being ineffective in relation to trauma extension both in time span (e.g., chronic diseases) and in size (e.g., large trauma). For these reasons, tissue …

mTOR Inhibition Leads to Src-Mediated EGFR Internalisation and Degradation in Glioma Cells Open

Barbara Colella, Mayra Colardo, Gianna Iannone, Claudia Contadini, Cristina Sáiz-Ladera , et al. · 2020

Epidermal Growth Factor receptor (EGFR) is a tyrosine kinase receptor widely expressed on the surface of numerous cell types, which activates several downstream signalling pathways involved in cell proliferation, migration and survival. EG…

High-Dimensional Single-Cell Quantitative Profiling of Skeletal Muscle Cell Population Dynamics during Regeneration Open

Lucia Lisa Petrilli, Filomena Spada, Alessandro Palma, Alessio Reggio, Marco Rosina , et al. · 2020

The interstitial space surrounding the skeletal muscle fibers is populated by a variety of mononuclear cell types. Upon acute or chronic insult, these cell populations become activated and initiate finely-orchestrated crosstalk that promot…

Biofabricating murine and human myo-substitutes for rapid volumetric muscle loss restoration Open

Marco Costantini, Stefano Testa, Ersilia Fornetti, Claudia Fuoco, Minghao Nie , et al. · 2020

The importance of skeletal muscle tissue is undoubted being the controller of several vital functions including respiration and all voluntary locomotion activities. However, its regenerative capability is limited and significant tissue los…

SCA-1 micro-heterogeneity in the fate decision of dystrophic fibro/adipogenic progenitors Open

Giulio Giuliani, Simone Vumbaca, Claudia Fuoco, Cesare Gargioli, Ezio Giorda , et al. · 2020

The term micro-heterogeneity refers to non-genetic cell to cell variability observed in a bell-shaped distribution of the expression of a trait within a population. The contribution of micro-heterogeneity to physiology and pathology remain…

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