Claudia Giesecke‐Thiel
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View article: Aging and Viral Evolution Impair Immunity Against Dominant Pan‐Coronavirus‐Reactive T Cell Epitope
Aging and Viral Evolution Impair Immunity Against Dominant Pan‐Coronavirus‐Reactive T Cell Epitope Open
Immune evasion by escape mutations subverts immunity against SARS‐CoV‐2. A role of pan‐coronavirus immunity for more durable protection is being discussed, but has remained understudied. We here investigated the effects of age, mutations, …
View article: CD8 <sup>+</sup> T cell–derived CD40L mediates noncanonical cytotoxicity in CD40-expressing cancer cells
CD8 <sup>+</sup> T cell–derived CD40L mediates noncanonical cytotoxicity in CD40-expressing cancer cells Open
T cells and their effector functions, in particular the canonical cytotoxicity of CD8 + T cells involving perforin, granzymes, Fas ligand (FasL), and tumor necrosis factor related apoptosis inducing ligand (TRAIL), are crucial for tumor im…
View article: Mesenchymal Osr1+ cells regulate embryonic lymphatic vessel formation
Mesenchymal Osr1+ cells regulate embryonic lymphatic vessel formation Open
The lymphatic system is formed during embryonic development by the commitment of specialized lymphatic endothelial cells (LECs) and their subsequent assembly in primary lymphatic vessels. Although lymphatic cells are in continuous contact …
View article: Aging and viral evolution impair immunity against dominant pan-coronavirus-reactive T cell epitope
Aging and viral evolution impair immunity against dominant pan-coronavirus-reactive T cell epitope Open
Immune evasion by escape mutations subverts immunity against SARS-CoV-2. A role of pan-coronavirus immunity for more durable protection is being discussed but has remained understudied. We here investigated the effects of age, mutations, a…
View article: “Mesenchymal Osr1+ cells regulate embryonic lymphatic vessel formation”
“Mesenchymal Osr1+ cells regulate embryonic lymphatic vessel formation” Open
The lymphatic system is formed during embryonic development by the commitment of specialized lymphatic endothelial cells (LECs) and their subsequent assembly in primary lymphatic vessels. While lymphatic cells are in continuous contact wit…
View article: Odd skipped-related 1 controls the pro-regenerative response of fibro-adipogenic progenitors
Odd skipped-related 1 controls the pro-regenerative response of fibro-adipogenic progenitors Open
Skeletal muscle regeneration requires the coordinated interplay of diverse tissue-resident- and infiltrating cells. Fibro-adipogenic progenitors (FAPs) are an interstitial cell population that provides a beneficial microenvironment for mus…
View article: Primary ChAdOx1 vaccination does not reactivate pre-existing, cross-reactive immunity
Primary ChAdOx1 vaccination does not reactivate pre-existing, cross-reactive immunity Open
Currently available COVID-19 vaccines include inactivated virus, live attenuated virus, mRNA-based, viral vectored and adjuvanted protein-subunit-based vaccines. All of them contain the spike glycoprotein as the main immunogen and result i…
View article: SARS-CoV-2 mRNA vaccinations fail to elicit humoral and cellular immune responses in patients with multiple sclerosis receiving fingolimod
SARS-CoV-2 mRNA vaccinations fail to elicit humoral and cellular immune responses in patients with multiple sclerosis receiving fingolimod Open
Background SARS-CoV-2 mRNA vaccination of healthy individuals is highly immunogenic and protective against severe COVID-19. However, there are limited data on how disease-modifying therapies (DMTs) alter SARS-CoV-2 mRNA vaccine immunogenic…
View article: Odd skipped-related 1 controls the pro-regenerative response of Fibro-Adipogenic Progenitors
Odd skipped-related 1 controls the pro-regenerative response of Fibro-Adipogenic Progenitors Open
Skeletal muscle regeneration requires the coordinated interplay of diverse tissue-resident- and infiltrating cells. Fibro-adipogenic progenitors (FAPs) are an interstitial cell population that provides a beneficial microenvironment for mus…
View article: SARS-CoV-2 mRNA vaccination fails to elicit humoral and cellular immune responses in multiple sclerosis patients receiving fingolimod
SARS-CoV-2 mRNA vaccination fails to elicit humoral and cellular immune responses in multiple sclerosis patients receiving fingolimod Open
SARS-CoV-2 mRNA vaccination of healthy individuals is highly immunogenic and protective against severe COVID-19. However, there are limited data on how disease-modifying therapies (DMTs) alter SARS-CoV-2 mRNA vaccine immunogenicity in pati…
View article: Cutting Edge: Serum but Not Mucosal Antibody Responses Are Associated with Pre-Existing SARS-CoV-2 Spike Cross-Reactive CD4+ T Cells following BNT162b2 Vaccination in the Elderly
Cutting Edge: Serum but Not Mucosal Antibody Responses Are Associated with Pre-Existing SARS-CoV-2 Spike Cross-Reactive CD4+ T Cells following BNT162b2 Vaccination in the Elderly Open
Advanced age is a main risk factor for severe COVID-19. However, low vaccination efficacy and accelerated waning immunity have been reported in this age group. To elucidate age-related differences in immunogenicity, we analyzed human cellu…
View article: Serum but not mucosal antibody responses are associated with pre-existing SARS-CoV-2 spike cross-reactive CD4<sup>+</sup>T cells following BNT162b2 vaccination in the elderly
Serum but not mucosal antibody responses are associated with pre-existing SARS-CoV-2 spike cross-reactive CD4<sup>+</sup>T cells following BNT162b2 vaccination in the elderly Open
Advanced age is a main risk factor for severe COVID-19. However, low vaccination efficacy and accelerated waning immunity have been reported in this age group. To elucidate age-related differences in immunogenicity, we analysed human cellu…
View article: Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification
Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification Open
Flow cytometers are robust and ubiquitous tools of biomedical research, as they enable high-throughput fluorescence-based multi-parametric analysis and sorting of single cells. However, analysis is often constrained by the availability of …
View article: Cross-reactive CD4 <sup>+</sup> T cells enhance SARS-CoV-2 immune responses upon infection and vaccination
Cross-reactive CD4 <sup>+</sup> T cells enhance SARS-CoV-2 immune responses upon infection and vaccination Open
Cross-reactive T cells aid in the fight There is mounting evidence that immunological memory after infection with seasonal human coronaviruses (hCoVs) contributes to cross-protection against severe acute respiratory syndrome coronavirus 2 …
View article: Dataset and Code for Manuscript "Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification"
Dataset and Code for Manuscript "Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification" Open
Dataset of measurements for cell cycle analysis with description: ReadMe file with explanations on the data set and analysis exemplary Matlab script file for analysis binary data files conatining the pulse shapes in all channels FCS data f…
View article: Dataset and Code for Manuscript "Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification"
Dataset and Code for Manuscript "Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification" Open
Dataset of measurements for cell cycle analysis with description: ReadMe file with explanations on the data set and analysis exemplary Matlab script file for analysis binary data files conatining the pulse shapes in all channels FCS data f…
View article: Cross-reactive CD4<sup>+</sup> T cells enhance SARS-CoV-2 immune responses upon infection and vaccination
Cross-reactive CD4<sup>+</sup> T cells enhance SARS-CoV-2 immune responses upon infection and vaccination Open
While evidence for pre-existing SARS-CoV-2-cross-reactive CD4 + T cells in unexposed individuals is increasing, their functional significance remains unclear. Here, we comprehensively determined SARS-CoV-2-cross-reactivity and human corona…
View article: Generation of HBsAg‐reactive T‐ and B‐cells following HBV vaccination in serological non‐responders under hemodialysis treatment
Generation of HBsAg‐reactive T‐ and B‐cells following HBV vaccination in serological non‐responders under hemodialysis treatment Open
HBV vaccination is recommend for hemodialysis patients, but only 50-60% of the patients show seroconversion. HBV vaccine-induced generation of HBV reactive T and B cells could be detected regardless of their capacity to mount a serological…
View article: Presence of SARS-CoV-2-reactive T cells in COVID-19 patients and healthy donors
Presence of SARS-CoV-2-reactive T cells in COVID-19 patients and healthy donors Open
Summary Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a rapidly unfolding pandemic, overwhelming health care systems worldwide 1 . Clinical manifestations of Coronavirus-disease 2019 (COVID-19) vary broadly, rangi…
View article: Human CD27+ memory B cells colonize a superficial follicular zone in the palatine tonsils with similarities to the spleen. A multicolor immunofluorescence study of lymphoid tissue
Human CD27+ memory B cells colonize a superficial follicular zone in the palatine tonsils with similarities to the spleen. A multicolor immunofluorescence study of lymphoid tissue Open
Background: Memory B cell (mBC) induction and maintenance is one of the keys to long-term protective humoral immunity. MBCs are fundamental to successful medical interventions such as vaccinations and therapy in autoimmunity. However, thei…
View article: Response to: Comments on the publication “Determination of Background, Signal‐to‐Noise, and Dynamic Range of a Flow Cytometer: A Novel Practical Method for Instrument Characterization and Standardization” by Giesecke et al.
Response to: Comments on the publication “Determination of Background, Signal‐to‐Noise, and Dynamic Range of a Flow Cytometer: A Novel Practical Method for Instrument Characterization and Standardization” by Giesecke et al. Open
In the September 2017 issue of Cytometry A, we have described the use of an LED pulser to characterize a flow cytometer's photodetector's response curve over a defined range of amplification, henceforth referred to as the signal-to-backgro…
View article: Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium
Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium Open
Oncogenic mutations in KRAS or BRAF are frequent in colorectal cancer and activate the ERK kinase. Here, we find graded ERK phosphorylation correlating with cell differentiation in patient-derived colorectal cancer organoids with and witho…
View article: Determination of background, signal‐to‐noise, and dynamic range of a flow cytometer: A novel practical method for instrument characterization and standardization
Determination of background, signal‐to‐noise, and dynamic range of a flow cytometer: A novel practical method for instrument characterization and standardization Open
A well‐defined scale calibration in flow cytometry can improve many aspects of data acquisition such as cytometer setup, instrument comparison and sample comparison. The theory for scale calibration was proposed by Steen over two decades a…