Claudio Tomi-Andrino
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View article: Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations
Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations Open
Base editors are recent multiplex gene editing tools derived from the Cas9 nuclease of Streptomyces pyogenes . They can target and modify a single nucleotide in the genome without inducing double-strand breaks (DSB) of the DNA helix. As su…
View article: Metabolic modeling-based drug repurposing in Glioblastoma
Metabolic modeling-based drug repurposing in Glioblastoma Open
View article: A genome-scale metabolic model of Cupriavidus necator H16 integrated with TraDIS and transcriptomic data reveals metabolic insights for biotechnological applications
A genome-scale metabolic model of Cupriavidus necator H16 integrated with TraDIS and transcriptomic data reveals metabolic insights for biotechnological applications Open
Exploiting biological processes to recycle renewable carbon into high value platform chemicals provides a sustainable and greener alternative to current reliance on petrochemicals. In this regard Cupriavidus necator H16 represents a partic…
View article: Required Gene Set for Autotrophic Growth of <i>Clostridium autoethanogenum</i>
Required Gene Set for Autotrophic Growth of <i>Clostridium autoethanogenum</i> Open
Although microbial genome sequences are relatively easily determined, assigning gene function remains a bottleneck. Consequently, relatively few genes are well characterized, leaving the function of many as either hypothetical or entirely …
View article: Application of transposon-insertion sequencing to determine gene essentiality in the acetogen <i>Clostridium autoethanogenum</i>
Application of transposon-insertion sequencing to determine gene essentiality in the acetogen <i>Clostridium autoethanogenum</i> Open
The majority of the genes present in bacterial genomes remain poorly characterised with up to one third of those that are protein encoding having no definitive function. Transposon insertion sequencing represents a high-throughput techniqu…
View article: Physicochemical and metabolic constraints for thermodynamics-based stoichiometric modelling under mesophilic growth conditions
Physicochemical and metabolic constraints for thermodynamics-based stoichiometric modelling under mesophilic growth conditions Open
Metabolic engineering in the post-genomic era is characterised by the development of new methods for metabolomics and fluxomics, supported by the integration of genetic engineering tools and mathematical modelling. Particularly, constraint…
View article: Physicochemical and metabolic constraints for thermodynamics-based stoichiometric modelling under mesophilic growth conditions
Physicochemical and metabolic constraints for thermodynamics-based stoichiometric modelling under mesophilic growth conditions Open
Metabolic engineering in the post-genomic era is characterised by the development of new methods for metabolomics and fluxomics, supported by the integration of genetic engineering tools and mathematical modelling. Particularly, constraint…