Explanipedia

Cooperative Clamp-Mediated Promoter Recognition by Poxviral RNAP and Its TBP/TFIIB-Like Partner Open

Utz Fischer, Stefan Jungwirth, J. Bartuli, Stephanie Lamer, Andreas Schlösser , et al. · 2025

The recruitment of RNA polymerase (RNAP) to gene promoters is a critical step in gene expression. For RNAP II, this process is initiated by the TATA-box binding protein (TBP) and transcription factor IIB (TFIIB), with homologs of these TBP…

tRNA as an assembly chaperone for a macromolecular transcription-processing complex Open

J. Bartuli, Stefan Jungwirth, Manisha Dixit, Takumi Okuda, Johannes P. Zimmermann , et al. · 2025

Whole-genome sequencing, as a powerful diagnostic tool in hearing loss, reveals novel variants in PTPRQ missed by whole-exome sequencing Open

Daniel Bengl, Asuman Koparır, Wahyu Eka Prastyo, Christian W. Remmele, Marcus Dittrich , et al. · 2025

Structure and flexibility of the DNA polymerase holoenzyme of vaccinia virus Open

W.P. Burmeister, Laetitia Boutin, Aurélia C. Balestra, Henri Gröger, Allison Ballandras-Colas , et al. · 2024

The year 2022 was marked by the mpox outbreak caused by the human monkeypox virus (MPXV), which is approximately 98% identical to the vaccinia virus (VACV) at the sequence level with regard to the proteins involved in DNA replication. We p…

The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP Open

Maria Körner, Susanne Meyer, Gabriella Marincola, Maximilian J Kern, Clemens Grimm , et al. · 2023

The ATPase p97 (also known as VCP, Cdc48) has crucial functions in a variety of important cellular processes such as protein quality control, organellar homeostasis, and DNA damage repair, and its de-regulation is linked to neuromuscular d…

Author response: The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP Open

Maria Körner, Susanne Meyer, Gabriella Marincola, Maximilian J Kern, Clemens Grimm , et al. · 2023

Full text Figures and data Side by side Abstract Editor's evaluation Introduction Results Discussion Materials and methods Appendix 1 Data availability References Decision letter Author response Article and author information Metrics Abstr…

Structure and flexibility of the DNA polymerase holoenzyme of vaccinia virus Open

W.P. Burmeister, Laetitia Boutin, Aurélia C. Balestra, Henri Gröger, Allison Ballandras-Colas , et al. · 2023

The year 2022 was marked by the mpox outbreak caused by human monkeypox virus (MPXV), which is about 98 % identical to vaccinia virus (VACV) at the sequence level regarding the proteins involved in DNA replication. We present the strategy …

FAM104 proteins promote the nuclear localization of p97/VCP Open

Maria Körner, Susanne Meyer, Gabriella Marincola, Maximilian J Kern, Clemens Grimm , et al. · 2023

The ATPase p97 (also known as VCP, Cdc48) has crucial functions in a variety of important cellular processes such as protein quality control, organellar homeostasis and DNA damage repair, and its de-regulation is linked to neuro-muscular d…

Developing protein structure figures Open

Clemens Grimm, Bin Liu, Vanessa J. Flegler, Jeong Joo Kim · 2023

The Vaccinia Virus DNA Helicase Structure from Combined Single-Particle Cryo-Electron Microscopy and AlphaFold2 Prediction Open

Stephanie Hutin, Wai Li Ling, Nicolas Tarbouriech, Guy Schoehn, Clemens Grimm , et al. · 2022

Poxviruses are large DNA viruses with a linear double-stranded DNA genome circularized at the extremities. The helicase-primase D5, composed of six identical 90 kDa subunits, is required for DNA replication. D5 consists of a primase fragme…

A generic protocol for the affinity-purification of native macromolecular complexes from poxvirus-infected cells Open

J. Bartuli, Isotta Lorenzi, Simone Backes, Clemens Grimm, Utz Fischer · 2022

The functional and structural characterization of macromolecular complexes requires protocols for their native isolation. Here, we describe a protocol for this task based on the recombinant poxvirus Vaccinia expressing tagged proteins of i…

Structural basis of the complete poxvirus transcription initiation process Open

Clemens Grimm, J. Bartuli, Bettina Boettcher, Aladar A. Szalay, Utz Fischer · 2021

Structural basis of the complete poxvirus transcription initiation process Open

Utz Fischer, Clemens Grimm, J. Bartuli, Bettina Böttcher, Aladar A. Szalay · 2021

Poxviruses express their genes in the cytoplasm of infected cells using a virus-encoded multi-subunit polymerase (vRNAP) and unique transcription factors. We present cryo-EM structures that uncover the complete transcription initiation of …

Additional causal <i>SNRPE</i> mutations in hereditary hypotrichosis simplex Open

Pan Cheng, Aytaj Humbatova, Lan Zheng, Nicole Cesarato, Clemens Grimm , et al. · 2021

Dear Editor, Hereditary hypotrichosis simplex (HHS) refers to a group of monogenic isolated alopecias characterized by diffuse and progressive loss of scalp and/or body hair. They start in childhood and progress with age. Affected individu…

Identification and structural analysis of the <i>Schizosaccharomyces pombe</i> SMN complex Open

Jyotishman Veepaschit, Aravindan Viswanathan, Rémy Bordonné, Clemens Grimm, Utz Fischer · 2021

The macromolecular SMN complex facilitates the formation of Sm-class ribonucleoproteins involved in mRNA processing (UsnRNPs). While biochemical studies have revealed key activities of the SMN complex, its structural investigation is laggi…

Crystal Structure of a Variant PAM2 Motif of LARP4B Bound to the MLLE Domain of PABPC1 Open

Clemens Grimm, J.P. Pelz, Cornelius Schneider, Katrin Schäffler, Utz Fischer · 2020

Eukaryotic cells determine the protein output of their genetic program by regulating mRNA transcription, localization, translation and turnover rates. This regulation is accomplished by an ensemble of RNA-binding proteins (RBPs) that bind …

A critical examination of the recently reported crystal structures of the human SMN protein Open

M.S. Weiss, Kay Diederichs, Randy J. Read, Santosh Panjikar, Gregory D. Van Duyne , et al. · 2020

A recent publication by Seng et al. in this journal reports the crystallographic structure of refolded, full-length SMN protein and two disease-relevant derivatives thereof. Here, we would like to suggest that at least two of the structure…

Structural Basis of Poxvirus Transcription: Vaccinia RNA Polymerase Complexes Open

Clemens Grimm, Hauke S. Hillen, Kristina Bedenk, J. Bartuli, Simon Neyer , et al. · 2019

A missense mutation in SNRPE linked to non-syndromal microcephaly interferes with U snRNP assembly and pre-mRNA splicing Open

Tao Chen, Bin Zhang, Thomas Ziegenhals, Archana Bairavasundaram Prusty, Sebastian Fröhler , et al. · 2019

Malfunction of pre-mRNA processing factors are linked to several human diseases including cancer and neurodegeneration. Here we report the identification of a de novo heterozygous missense mutation in the SNRPE gene (c.65T>C (p.Phe22Ser)) …

Reversibility of a Point Mutation Induced Domain Shift: Expanding the Conformational Space of a Sucrose Phosphorylase Open

Michael Kraus, Clemens Grimm, Jürgen Seibel · 2018

Despite their popularity as enzyme engineering targets structural information about Sucrose Phosphorylases remains scarce. We recently clarified that the Q345F variant of Bifidobacterium adolescentis Sucrose Phosphorylase is able to accept…

The conserved p.Arg108 residue in S1PR2 (DFNB68) is fundamental for proper hearing: evidence from a consanguineous Iranian family Open

Michaela A. H. Hofrichter, Majid Mojarad, Julia Doll, Clemens Grimm, Atiye Eslahi , et al. · 2018

Near-Atomic Resolution Structure of a Plant Geminivirus Determined by Electron Cryomicroscopy Open

Katharina Hipp, Clemens Grimm, Holger Jeske, Bettina Böttcher · 2017

Exploring the Structural Space of the Galectin‐1–Ligand Interaction Open

Nadja Bertleff‐Zieschang, Julian Bechold, Clemens Grimm, Michael Reutlinger, Petra Schneider , et al. · 2017

Galectin‐1 is a tumor‐associated protein recognizing the Galβ1‐4GlcNAc motif of cell‐surface glycoconjugates. Herein, we report the stepwise expansion of a multifunctional natural scaffold based on N ‐acetyllactosamine (Lac N Ac). We obtai…

Proteomic Analysis of Excretory-Secretory Products of Mesocestoides corti Metacestodes Reveals Potential Suppressors of Dendritic Cell Functions Open

Emilia Vendelova, Jeferson Camargo de Lima, Karina Rodrigues Lorenzatto, Karina Mariante Monteiro, Thomas D. Mueller , et al. · 2016

Accumulating evidences have assigned a central role to parasite-derived proteins in immunomodulation. Here, we report on the proteomic identification and characterization of immunomodulatory excretory-secretory (ES) products from the metac…

A critical examination of the recently reported crystal structures of the human SMN protein Open

M.S. Weiss, Kay Diederichs, Randy J. Read, Santosh Panjikar, Gregory D. Van Duyne , et al. · 2016

A recent publication by Seng et al. in this journal reports the crystallographic structure of refolded, full-length SMN protein and two disease-relevant derivatives thereof. Here, we would like to suggest that at least two of the structure…

Cover Picture: Redesign of the Active Site of Sucrose Phosphorylase through a Clash‐Induced Cascade of Loop Shifts (ChemBioChem 1/2016) Open

Michael Kraus, Clemens Grimm, Jürgen Seibel · 2016

The cover picture shows the active site and entrance channel of the Q345F variant of the sucrose phosphorylase from Bifidobacterium adolescentis (BaSP, PDB ID: 5C8B). Reshaping enzyme pockets paves the way for new enzyme activities. BaSP i…

Crystallizing the 6S and 8S spliceosomal assembly intermediates: a complex project Open

J.P. Pelz, Hermann Schindelin, Katharina van Pee, Jochen Kuper, Caroline Kisker , et al. · 2015

The small nuclear ribonucleoproteins (snRNPs) U1, U2, U4/6 and U5 are major constituents of the pre-mRNA processing spliceosome. They contain a common RNP core that is formed by the ordered binding of Sm proteins onto the single-stranded S…

ProteoPlex: stability optimization of macromolecular complexes by sparse-matrix screening of chemical space Open

Ashwin Chari, David Haselbach, Jan-Martin Kirves, Juergen Ohmer, Elham Paknia , et al. · 2015

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