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View article: Pro-inflammatory response of human iPSC-derived intestinal epithelial monolayers towards microbial toxins LPS and nigericin
Pro-inflammatory response of human iPSC-derived intestinal epithelial monolayers towards microbial toxins LPS and nigericin Open
The intestinal epithelium forms a selective barrier between the intestinal lumen and the subepithelial layer. Intestinal epithelium plays a critical role in initiating inflammatory tissue responses in vivo, which remains challenging to emu…
View article: Induction of endotoxin tolerance in murine monocyte and macrophage cell populations – optimal LPS dose and compartment-specific reversal by β-glucan
Induction of endotoxin tolerance in murine monocyte and macrophage cell populations – optimal LPS dose and compartment-specific reversal by β-glucan Open
Dietary β-glucan has the potential to selectively counteract LPS-induced immunosuppression.
View article: Dietary Intake of Yeast‐Derived β‐Glucan and Rice‐Derived Arabinoxylan Induces Dose‐Dependent Innate Immune Priming in Mice
Dietary Intake of Yeast‐Derived β‐Glucan and Rice‐Derived Arabinoxylan Induces Dose‐Dependent Innate Immune Priming in Mice Open
Beta‐glucans and arabinoxylans are known for their immunostimulatory properties. However, in vivo these have been documented almost exclusively following parenteral administration, underemphasizing oral intake. C57BL/6 mice are fed either …
View article: Dietary fat induced chylomicron-mediated LPS translocation in a bicameral Caco-2cell model
Dietary fat induced chylomicron-mediated LPS translocation in a bicameral Caco-2cell model Open
Background There is increasing evidence that dietary fat, especially saturated fat, promotes the translocation of lipopolysaccharide (LPS) via chylomicron production in the gut. Chylomicrons can subsequently transport LPS to other parts of…
View article: Methanogen Levels Are Significantly Associated with Fecal Microbiota Composition and Alpha Diversity in Healthy Adults and Irritable Bowel Syndrome Patients
Methanogen Levels Are Significantly Associated with Fecal Microbiota Composition and Alpha Diversity in Healthy Adults and Irritable Bowel Syndrome Patients Open
Hydrogenotrophic microbes play an essential role in the disposal of hydrogen and the maintenance of the hydrogen balance in gut ecosystems. Their abundances vary between individuals and have been reported to be associated with human gut di…
View article: Dietary fat enhanced chylomicron-mediated LPS translocation in a Caco-2 transwell model
Dietary fat enhanced chylomicron-mediated LPS translocation in a Caco-2 transwell model Open
Background There is increasing evidence that dietary fat, and especially saturated fat, promotes the absorption of lipopolysaccharide (LPS) via enhanced chylomicron production. The latter can subsequently transport LPS to other parts of th…
View article: Fecal Microbiota Signatures Are Not Consistently Related to Symptom Severity in Irritable Bowel Syndrome
Fecal Microbiota Signatures Are Not Consistently Related to Symptom Severity in Irritable Bowel Syndrome Open
Fecal microbiota and SCFA signatures were not consistently associated with IBS severity over time, indicating the importance of repeated sampling in IBS research.
View article: Dietary fat enhanced chylomicron-mediated LPS translocation in a Caco-2 transwell model
Dietary fat enhanced chylomicron-mediated LPS translocation in a Caco-2 transwell model Open
Background There is increasing evidence that dietary fat, and especially saturated fat, promotes the absorption of lipopolysaccharide (LPS) via enhanced chylomicron production. The latter can subsequently transport LPS to other parts of th…
View article: Dectin-1b activation by arabinoxylans induces trained immunity in human monocyte-derived macrophages
Dectin-1b activation by arabinoxylans induces trained immunity in human monocyte-derived macrophages Open
Arabinoxylans of various structures and sources have shown to possess the ability to induce a range of immune responses in different cell types in vitro and in vivo. Although the underlying mechanisms remain to be fully established, severa…
View article: Ingestion, Immunity, and Infection: Nutrition and Viral Respiratory Tract Infections
Ingestion, Immunity, and Infection: Nutrition and Viral Respiratory Tract Infections Open
Respiratory infections place a heavy burden on the health care system, particularly in the winter months. Individuals with a vulnerable immune system, such as very young children and the elderly, and those with an immune deficiency, are at…
View article: Bioactive properties of insect products for monogastric animals – a review
Bioactive properties of insect products for monogastric animals – a review Open
Insects or products derived from insects are considered as a valuable feed ingredient for aquaculture, poultry, and pigs but also exert possible secondary effects of health stimulators in livestock as reported in literature. Health benefit…
View article: Continuous Exposure to Non-Soluble β-Glucans Induces Trained Immunity in M-CSF-Differentiated Macrophages
Continuous Exposure to Non-Soluble β-Glucans Induces Trained Immunity in M-CSF-Differentiated Macrophages Open
Beta-glucans enable functional reprogramming of innate immune cells, a process defined as “trained immunity”, which results in enhanced host responsiveness against primary (training) and/or secondary infections (resilience). Trained immuni…
View article: Subtypes and Severity of Irritable Bowel Syndrome Are Not Related to Patients’ Self-Reported Dietary Triggers: Results From an Online Survey in Dutch Adults
Subtypes and Severity of Irritable Bowel Syndrome Are Not Related to Patients’ Self-Reported Dietary Triggers: Results From an Online Survey in Dutch Adults Open
Patients with different IBS subtypes or IBS severity do not identify different self-reported dietary triggers. Patients with more severe IBS and who experience anxiety or depression tend to have severe responses to more dietary triggers. I…
View article: A THP-1 Cell Line-Based Exploration of Immune Responses Toward Heat-Treated BLG
A THP-1 Cell Line-Based Exploration of Immune Responses Toward Heat-Treated BLG Open
Allergen recognition and processing by antigen presenting cells is essential for the sensitization step of food allergy. Macrophages and dendritic cells are both phagocytic antigen presenting cells and play important roles in innate immune…
View article: <i>Clostridioides difficile</i> toxin A-mediated Caco-2 cell barrier damage was attenuated by insect-derived fractions and corresponded to increased gene transcription of cell junctional and proliferation proteins
<i>Clostridioides difficile</i> toxin A-mediated Caco-2 cell barrier damage was attenuated by insect-derived fractions and corresponded to increased gene transcription of cell junctional and proliferation proteins Open
Protein-enriched insect fractions mitigated Clostridium difficile toxin A-mediated Caco-2 barrier damage by increasing gene transcription of cell junctional and proliferation proteins.
View article: In Vitro Studies Toward the Use of Chitin as Nutraceutical: Impact on the Intestinal Epithelium, Macrophages, and Microbiota
In Vitro Studies Toward the Use of Chitin as Nutraceutical: Impact on the Intestinal Epithelium, Macrophages, and Microbiota Open
Scope Chitin, the most abundant polysaccharide found in nature after cellulose, is known for its ability to support wound healing and to lower plasma‐oxidized low‐density lipoprotein (LDL) levels. Studies have also revealed immunomodulator…
View article: Novel Dietary Proteins Selectively Affect Intestinal Health In Vitro after Clostridium difficile-Secreted Toxin A Exposure
Novel Dietary Proteins Selectively Affect Intestinal Health In Vitro after Clostridium difficile-Secreted Toxin A Exposure Open
Bacterial gastroenteritis forms a burden on a global scale, both socially and economically. The Gram-positive bacterium Clostridium difficile is an inducer of gastrointestinal bacterial infections, often triggered following disruption of t…
View article: Hydrophobicity drives receptor-mediated uptake of heat-processed proteins by THP-1 macrophages and dendritic cells, but not cytokine responses
Hydrophobicity drives receptor-mediated uptake of heat-processed proteins by THP-1 macrophages and dendritic cells, but not cytokine responses Open
Although an impact of processing on immunogenicity of food proteins has clearly been demonstrated, the underlying mechanisms are still unclear. We applied 3 different processing methods: wet heating (60 °C) and low- or high-temperature (50…
View article: Hydrophobicity drives receptor-mediated uptake of heat-processed proteins by THP-1 macrophages and dendritic cells, but not cytokine responses
Hydrophobicity drives receptor-mediated uptake of heat-processed proteins by THP-1 macrophages and dendritic cells, but not cytokine responses Open
Impact of processing on immunogenicity of food proteins has clearly been demonstrated, but the underlying mechanisms are still unclear. In our previous study, the uptake of the cow’s milk protein β-lactoglobulin (BLG) by THP-1 macrophages …
View article: Wheat-derived arabinoxylans reduced M2-macrophage functional activity, but enhanced monocyte-recruitment capacity
Wheat-derived arabinoxylans reduced M2-macrophage functional activity, but enhanced monocyte-recruitment capacity Open
Various non-digestible polysaccharides (NDPs), in particular wheat-AX, altered the gene transcription, function, cytokine and chemokine secretion pattern of non-polarized M0 and M1- and M2-like macrophages.
View article: Hydrophobicity and aggregation, but not glycation, are key determinants for uptake of thermally processed β-lactoglobulin by THP-1 macrophages
Hydrophobicity and aggregation, but not glycation, are key determinants for uptake of thermally processed β-lactoglobulin by THP-1 macrophages Open
The aim of this study is to investigate the immunological relevance of modifications of food protein structure due to thermal processing. We investigated the uptake of β-lactoglobulin, treated with 3 different processing methods, by THP-1 …
View article: Consumption of β-glucans to spice up T cell treatment of tumors: a review
Consumption of β-glucans to spice up T cell treatment of tumors: a review Open
Beta-glucans, when present in food and following uptake in the proximal gut, stimulate immune cells present in gut-associated lymphoid tissue and initiate highly conserved pro-inflammatory pathways. When tested in mouse cancer models, β-gl…
View article: The Effect of Tomatine on Gene Expression and Cell Monolayer Integrity in Caco-2
The Effect of Tomatine on Gene Expression and Cell Monolayer Integrity in Caco-2 Open
More understanding of the risk-benefit effect of the glycoalkaloid tomatine is required to be able to estimate the role it might play in our diet. In this work, we focused on effects towards intestinal epithelial cells based on a Caco-2 mo…
View article: Macrophages treated with non-digestible polysaccharides reveal a transcriptionally unique phenotype
Macrophages treated with non-digestible polysaccharides reveal a transcriptionally unique phenotype Open
Dietary non-digestible polysaccharides (NDPs) might promote intestinal health via immuno-modulation. Immunomodulatory effects of NDP are most likely brought about by antigen processing cells such as macrophages that populate the intestine,…
View article: Optimized Triton X-114 assisted lipopolysaccharide (LPS) removal method reveals the immunomodulatory effect of food proteins
Optimized Triton X-114 assisted lipopolysaccharide (LPS) removal method reveals the immunomodulatory effect of food proteins Open
SCOPE:Investigations into the immunological response of proteins is often masked by lipopolysaccharide (LPS) contamination. We report an optimized Triton X-114 (TX-114) based LPS extraction method for β-lactoglobulin (BLG) and soy protein …
View article: Effects of Digested Onion Extracts on Intestinal Gene Expression: An Interspecies Comparison Using Different Intestine Models
Effects of Digested Onion Extracts on Intestinal Gene Expression: An Interspecies Comparison Using Different Intestine Models Open
Human intestinal tissue samples are barely accessible to study potential health benefits of nutritional compounds. Numbers of animals used in animal trials, however, need to be minimalized. Therefore, we explored the applicability of in vi…