Cole A. DeForest
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View article: From sequence to scaffold: Computational design of protein nanoparticle vaccines from AlphaFold2-predicted building blocks
From sequence to scaffold: Computational design of protein nanoparticle vaccines from AlphaFold2-predicted building blocks Open
Self-assembling protein nanoparticles are being increasingly utilized in the design of next-generation vaccines due to their ability to induce antibody responses of superior magnitude, breadth, and durability. Computational protein design …
View article: Suspended Tissue Engineering with Assemblable Microfluidics (STEAM)
Suspended Tissue Engineering with Assemblable Microfluidics (STEAM) Open
Suspended tissue culture systems enable cellular responses to mechanical forces critical for tissue development and function. Tissues develop in a complex environment containing both mechanical and chemical cues that vary spatiotemporally;…
View article: PhoCoil: A photodegradable and injectable single-component recombinant protein hydrogel for minimally invasive delivery and degradation
PhoCoil: A photodegradable and injectable single-component recombinant protein hydrogel for minimally invasive delivery and degradation Open
Hydrogel biomaterials offer great promise for three-dimensional cell culture and therapeutic delivery. Despite many successes, challenges persist in that gels formed from natural proteins are only marginally tunable whereas those derived f…
View article: From sequence to scaffold: computational design of protein nanoparticle vaccines from AlphaFold2-predicted building blocks
From sequence to scaffold: computational design of protein nanoparticle vaccines from AlphaFold2-predicted building blocks Open
Self-assembling protein nanoparticles are being increasingly utilized in the design of next-generation vaccines due to their ability to induce antibody responses of superior magnitude, breadth, and durability. Computational protein design …
View article: De Novo Design of Integrin α5β1 Modulating Proteins to Enhance Biomaterial Properties
De Novo Design of Integrin α5β1 Modulating Proteins to Enhance Biomaterial Properties Open
Integrin α5β1 is crucial for cell attachment and migration in development and tissue regeneration, and α5β1 binding proteins can have considerable utility in regenerative medicine and next‐generation therapeutics. We use computational prot…
View article: Suspended Tissue Open Microfluidic Patterning (STOMP)
Suspended Tissue Open Microfluidic Patterning (STOMP) Open
Free‐standing tissue structures tethered between pillars are powerful mechanobiology tools for studying cell contraction. To model interfaces ubiquitous in natural tissues and upgrade existing single‐region suspended constructs, we develop…
View article: Rapid and Inexpensive Image‐Guided Grayscale Biomaterial Customization via <scp>LCD</scp> Printing
Rapid and Inexpensive Image‐Guided Grayscale Biomaterial Customization via <span>LCD</span> Printing Open
Hydrogels are an important class of biomaterials that permit cells to be cultured and studied within engineered microenvironments of user‐defined physical and chemical properties. Though conventional 3D extrusion and stereolithographic (SL…
View article: Suspended Tissue Open Microfluidic Patterning (STOMP)
Suspended Tissue Open Microfluidic Patterning (STOMP) Open
Free-standing tissue structures tethered between pillars are powerful mechanobiology tools for studying cell contraction. To model interfaces ubiquitous in natural tissues and upgrade existing single-region suspended constructs, we develop…
View article: Bioluminescence Resonance Energy Transfer (BRET)-Mediated Protein Release from Self-Illuminating Photoresponsive Biomaterials
Bioluminescence Resonance Energy Transfer (BRET)-Mediated Protein Release from Self-Illuminating Photoresponsive Biomaterials Open
Phototriggered release of various cargos, including soluble protein factors and small molecules, has the potential to correct aberrant biological events by offering spatiotemporal control over local therapeutic levels. However, the poor pe…
View article: Stepwise Stiffening/Softening of and Cell Recovery from Reversibly Formulated Hydrogel Interpenetrating Networks
Stepwise Stiffening/Softening of and Cell Recovery from Reversibly Formulated Hydrogel Interpenetrating Networks Open
Biomechanical contributions of the extracellular matrix underpin cell growth and proliferation, differentiation, signal transduction, and other fate decisions. As such, biomaterials whose mechanics can be spatiotemporally altered‐ particul…
View article: De Novo Design of Integrin α5β1 Modulating Proteins for Regenerative Medicine
De Novo Design of Integrin α5β1 Modulating Proteins for Regenerative Medicine Open
Summary Integrin α5β1 is crucial for cell attachment and migration in development and tissue regeneration, and α5β1 binding proteins could have considerable utility in regenerative medicine and next-generation therapeutics. We use computat…
View article: Genetically Encoded XTEN‐based Hydrogels with Tunable Viscoelasticity and Biodegradability for Injectable Cell Therapies (Adv. Sci. 24/2024)
Genetically Encoded XTEN‐based Hydrogels with Tunable Viscoelasticity and Biodegradability for Injectable Cell Therapies (Adv. Sci. 24/2024) Open
Injectable Cell Therapies Single‐component protein hydrogels assembled through homopentameric coiled‐coil bundling exhibit rapid shear thinning and self‐healing, protecting and sustaining viability of encapsulated cells through culture, in…
View article: One-Step Purification and N-Terminal Functionalization of Bioactive Proteins via Atypically Split Inteins
One-Step Purification and N-Terminal Functionalization of Bioactive Proteins via Atypically Split Inteins Open
Site-specific installation of non-natural functionality onto proteins has enabled countless applications in biotechnology, chemical biology, and biomaterials science. Though the N-terminus is an attractive derivatization location, prior me…
View article: PhoCoil: An Injectable and Photodegradable Single-component Recombinant Protein Hydrogel for Localized Therapeutic Cell Delivery
PhoCoil: An Injectable and Photodegradable Single-component Recombinant Protein Hydrogel for Localized Therapeutic Cell Delivery Open
Hydrogel biomaterials offer great promise for 3D cell culture and therapeutic delivery. Despite many successes, challenges persist in that gels formed from natural proteins are only marginally tunable while those derived from synthetic pol…
View article: Versatile tissue‐injectable hydrogels capable of the extended hydrolytic release of bioactive protein therapeutics
Versatile tissue‐injectable hydrogels capable of the extended hydrolytic release of bioactive protein therapeutics Open
Hydrogels are extensively employed in healthcare due to their adaptable structures, high water content, and biocompatibility, with FDA‐approved applications ranging from spinal cord regeneration to local therapeutic delivery. However, clin…
View article: Stepwise Stiffening/Softening of and Cell Recovery from Reversibly Formulated Hydrogel Double Networks
Stepwise Stiffening/Softening of and Cell Recovery from Reversibly Formulated Hydrogel Double Networks Open
Biomechanical contributions of the ECM underpin cell growth and proliferation, differentiation, signal transduction, and other fate decisions. As such, biomaterials whose mechanics can be spatiotemporally altered – particularly in a revers…
View article: Genetically Encoded XTEN‐based Hydrogels with Tunable Viscoelasticity and Biodegradability for Injectable Cell Therapies
Genetically Encoded XTEN‐based Hydrogels with Tunable Viscoelasticity and Biodegradability for Injectable Cell Therapies Open
While direct cell transplantation holds great promise in treating many debilitating diseases, poor cell survival and engraftment following injection have limited effective clinical translation. Though injectable biomaterials offer protecti…
View article: Grayscale 4D Biomaterial Customization at High Resolution and Scale
Grayscale 4D Biomaterial Customization at High Resolution and Scale Open
Hydrogel biomaterials have proven indispensable for three-dimensional (3D) cell culture but have fallen short in replicating the innate physiochemical complexity of real tissue. Though traditional photolithography affords localized materia…
View article: De novo design of modular protein hydrogels with programmable intra- and extracellular viscoelasticity
De novo design of modular protein hydrogels with programmable intra- and extracellular viscoelasticity Open
Relating the macroscopic properties of protein-based materials to their underlying component microstructure is an outstanding challenge. Here, we exploit computational design to specify the size, flexibility, and valency of de novo protein…
View article: Irreversible light-activated SpyLigation mediates split-protein assembly in 4D
Irreversible light-activated SpyLigation mediates split-protein assembly in 4D Open
View article: Engineering native biological complexity from the inside–out and outside–in
Engineering native biological complexity from the inside–out and outside–in Open
View article: Chemical and biological engineering strategies to make and modify next-generation hydrogel biomaterials
Chemical and biological engineering strategies to make and modify next-generation hydrogel biomaterials Open
View article: Versatile Tissue-Injectable Hydrogels with Extended Hydrolytic Release of Bioactive Protein Therapeutics
Versatile Tissue-Injectable Hydrogels with Extended Hydrolytic Release of Bioactive Protein Therapeutics Open
Hydrogels generally have broad utilization in healthcare due to their tunable structures, high water content, and inherent biocompatibility. FDA-approved applications of hydrogels include spinal cord regeneration, skin fillers, and local t…
View article: Tricolor visible wavelength-selective photodegradable hydrogel biomaterials
Tricolor visible wavelength-selective photodegradable hydrogel biomaterials Open
Photodynamic hydrogel biomaterials have demonstrated great potential for user-triggered therapeutic release, patterned organoid development, and four-dimensional control over advanced cell fates in vitro. Current photosensitive materials a…
View article: 4D Biochemical Photocustomization of Hydrogel Scaffolds for Biomimetic Tissue Engineering
4D Biochemical Photocustomization of Hydrogel Scaffolds for Biomimetic Tissue Engineering Open
Programmable engineered tissues and the materials that support them are instrumental to the development of next-generation therapeutics and gaining new understanding of human biology. Toward these ends, recent years have brought a growing …
View article: De novo design of modular protein hydrogels with programmable intra- and extracellular viscoelasticity
De novo design of modular protein hydrogels with programmable intra- and extracellular viscoelasticity Open
Relating the macroscopic properties of protein-based materials to their underlying component microstructure is an outstanding challenge. Here, we exploit computational design to specify the size, flexibility, and valency of de novo protein…
View article: User‐Controlled 4D Biomaterial Degradation with Substrate‐Selective Sortase Transpeptidases for Single‐Cell Biology (Adv. Mater. 19/2023)
User‐Controlled 4D Biomaterial Degradation with Substrate‐Selective Sortase Transpeptidases for Single‐Cell Biology (Adv. Mater. 19/2023) Open
Multiplexed Cell Capture Harvesting cells from biomaterials for downstream analysis without perturbing their state represents an outstanding challenge in 3D/4D culture and tissue engineering. In article number 2209904, Cole A. DeForest and…
View article: Spatiotemporal functional assembly of split protein pairs through a light-activated SpyLigation
Spatiotemporal functional assembly of split protein pairs through a light-activated SpyLigation Open
View article: Highly Parallel Tissue Grafting for Combinatorial In Vivo Screening
Highly Parallel Tissue Grafting for Combinatorial In Vivo Screening Open
Material- and cell-based technologies such as engineered tissues hold great promise as human therapies. Yet, the development of many of these technologies becomes stalled at the stage of pre-clinical animal studies due to the tedious and l…
View article: User‐Controlled 4D Biomaterial Degradation with Substrate‐Selective Sortase Transpeptidases for Single‐Cell Biology
User‐Controlled 4D Biomaterial Degradation with Substrate‐Selective Sortase Transpeptidases for Single‐Cell Biology Open
Stimuli‐responsive biomaterials show great promise for modeling disease dynamics ex vivo with spatiotemporal control over the cellular microenvironment. However, harvesting cells from such materials for downstream analysis without perturbi…