Connor Dufort
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View article: Author Correction: Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and human brain
Author Correction: Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and human brain Open
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View article: IL-33/ST2 signaling in monocyte-derived macrophages maintains blood-brain barrier integrity and restricts infarctions early after ischemic stroke
IL-33/ST2 signaling in monocyte-derived macrophages maintains blood-brain barrier integrity and restricts infarctions early after ischemic stroke Open
Our results demonstrate that early-stage IL-33/ST2 signaling in infiltrating macrophages reduces the extent of acute BBB disruption after stroke. Intranasal IL-33 administration may represent a new strategy to reduce BBB leakage and infarc…
View article: Microglia: Active participants in brain capillary function
Microglia: Active participants in brain capillary function Open
A recent study by Bisht, Okojie, and Sharma, et al. characterizes a population of capillary-associated microglia (CAM) whose cell bodies are positioned along small blood vessels in the healthy mouse brain. Through elegant, longitudinal int…
View article: Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice
Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice Open
Immunomodulation holds therapeutic promise against brain injuries, but leveraging this approach requires a precise understanding of mechanisms. We report that CD8+CD122+CD49dlo T regulatory-like cells (CD8+ TRLs) are among the earliest lym…
View article: Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and human brain
Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and human brain Open
A key aspect of nearly all single-cell sequencing experiments is dissociation of intact tissues into single-cell suspensions. While many protocols have been optimized for optimal cell yield, they have often overlooked the effects that diss…
View article: A RIPK1-regulated inflammatory microglial state in amyotrophic lateral sclerosis
A RIPK1-regulated inflammatory microglial state in amyotrophic lateral sclerosis Open
Significance Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease. Inhibition of RIPK1, a kinase which regulates cell death and neuroinflammation, has been efficacious in treating mouse models of ALS. RIPK1 inhibi…
View article: Single Cell Sequencing Reveals Glial Specific Responses to Tissue Processing & Enzymatic Dissociation in Mice and Humans
Single Cell Sequencing Reveals Glial Specific Responses to Tissue Processing & Enzymatic Dissociation in Mice and Humans Open
A key aspect of nearly all single cell experiments is the necessity to dissociate intact tissues into single cell suspensions for processing. While many protocols have been optimized for optimal cell yield, they have often overlooked the e…
View article: Single-Cell RNA Sequencing of Microglia throughout the Mouse Lifespan and in the Injured Brain Reveals Complex Cell-State Changes
Single-Cell RNA Sequencing of Microglia throughout the Mouse Lifespan and in the Injured Brain Reveals Complex Cell-State Changes Open
View article: Complex cell-state changes revealed by single cell RNA sequencing of 76,149 microglia throughout the mouse lifespan and in the injured brain
Complex cell-state changes revealed by single cell RNA sequencing of 76,149 microglia throughout the mouse lifespan and in the injured brain Open
Microglia, the resident immune cells of the brain, rapidly change states in response to their environment, but we lack molecular and functional signatures of different microglial populations. In this study, we analyzed the RNA expression p…