Craig A. Lygate
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View article: Cardiac Energetics in Severe Mitral Regurgitation; Relationship with Eccentric Hypertrophy, Stroke Volume and Effects of Valve Repair
Cardiac Energetics in Severe Mitral Regurgitation; Relationship with Eccentric Hypertrophy, Stroke Volume and Effects of Valve Repair Open
Aims Understanding changes in ATP metabolism may lead to improved risk stratification in severe primary mitral regurgitation (MR). Here we seek to compare the energetic phenotype of volume-overload pathological hypertrophy with athletic hy…
View article: Targeting Troponin I Phosphorylation for Precision Treatment of Diastolic Dysfunction
Targeting Troponin I Phosphorylation for Precision Treatment of Diastolic Dysfunction Open
Background Defective cardiac relaxation (diastolic dysfunction) is prevalent in heart failure, particularly in the context of diabetes, obesity, hypertension, and ageing, and is associated with increased mortality. Yet no effective therapy…
View article: Nitric oxide releasing graphene for next-generation therapeutics
Nitric oxide releasing graphene for next-generation therapeutics Open
Nitric oxide (NO) is a powerful signalling molecule and plays a central role in numerous physiological processes, most notably, in the cardiovascular, immune and central nervous systems. While organic nitrates, exemplified by nitroglycerin…
View article: Effect of whole-body creatine-deficiency on secretion of insulin and glucagon from isolated mouse islets
Effect of whole-body creatine-deficiency on secretion of insulin and glucagon from isolated mouse islets Open
View article: Cardiac function and energetics in mice with combined genetic augmentation of creatine and creatine kinase activity
Cardiac function and energetics in mice with combined genetic augmentation of creatine and creatine kinase activity Open
Improving energy provision in the failing heart by augmenting the creatine kinase (CK) system is a desirable therapeutic target. However, over-expression of the creatine transporter (CrT-OE) has shown that very high creatine levels result …
View article: Synthesis and characterization of amine-functionalized graphene as a nitric oxide-generating coating for vascular stents
Synthesis and characterization of amine-functionalized graphene as a nitric oxide-generating coating for vascular stents Open
Drug-eluting stents are commonly utilized for the treatment of coronary artery disease, where they maintain vessel patency and prevent restenosis. However, problems with prolonged vascular healing, late thrombosis, and neoatherosclerosis p…
View article: Hyperpolarized <sup>13</sup>C and <sup>31</sup>P MRS detects differences in cardiac energetics, metabolism, and function in obesity, and responses following treatment
Hyperpolarized <sup>13</sup>C and <sup>31</sup>P MRS detects differences in cardiac energetics, metabolism, and function in obesity, and responses following treatment Open
Obesity is associated with important changes in cardiac energetics and function, and an increased risk of adverse cardiovascular outcomes. Multi‐nuclear MRS and MRI techniques have the potential to provide a comprehensive non‐invasive asse…
View article: pH-sensitive release of nitric oxide gas using peptide-graphene co-assembled hybrid nanosheets
pH-sensitive release of nitric oxide gas using peptide-graphene co-assembled hybrid nanosheets Open
View article: S-nitrosocysteamine-functionalised porous graphene oxide nanosheets as nitric oxide delivery vehicles for cardiovascular applications
S-nitrosocysteamine-functionalised porous graphene oxide nanosheets as nitric oxide delivery vehicles for cardiovascular applications Open
View article: Maintaining energy provision in the heart: the creatine kinase system in ischaemia–reperfusion injury and chronic heart failure
Maintaining energy provision in the heart: the creatine kinase system in ischaemia–reperfusion injury and chronic heart failure Open
The non-stop provision of chemical energy is of critical importance to normal cardiac function, requiring the rapid turnover of ATP to power both relaxation and contraction. Central to this is the creatine kinase (CK) phosphagen system, wh…
View article: Mitochondria-Targeted Nanomedicines for Cardiovascular Applications
Mitochondria-Targeted Nanomedicines for Cardiovascular Applications Open
Tweetable abstract Mitochondria are increasingly a target for drug delivery in cardiovascular diseases. This editorial describes how a nanomedicine approach may improve drug potency and efficacy in a safe and controlled manner.
View article: Graphene nanocomposites for real-time electrochemical sensing of nitric oxide in biological systems
Graphene nanocomposites for real-time electrochemical sensing of nitric oxide in biological systems Open
Nitric oxide (NO) signaling plays many pivotal roles impacting almost every organ function in mammalian physiology, most notably in cardiovascular homeostasis, inflammation, and neurological regulation. Consequently, the ability to make re…
View article: Nitric Oxide Releasing Nanomaterials for Cardiovascular Applications
Nitric Oxide Releasing Nanomaterials for Cardiovascular Applications Open
View article: Measuring cardiomyocyte cellular characteristics in cardiac hypertrophy using diffusion‐weighted MRI
Measuring cardiomyocyte cellular characteristics in cardiac hypertrophy using diffusion‐weighted MRI Open
Purpose This paper presents a hierarchical modeling approach for estimating cardiomyocyte major and minor diameters and intracellular volume fraction (ICV) using diffusion‐weighted MRI (DWI) data in ex vivo mouse hearts. Methods DWI data w…
View article: Supplementary Figure 2 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism
Supplementary Figure 2 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism Open
S2. (A) Kaplan-Meier analysis of overall survival of 440 colon adenocarcinoma patients TGCA cohort. Patient group with highest quartile showed a reduced five-year survival. (B) Heatmap illustrating the correlative gene expression profile o…
View article: Supplementary Figure 8 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism
Supplementary Figure 8 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism Open
S8. Hydrophilic metabolites detection and identification performed by LC/MS QTOF nanoflow using AMRT comparison.
View article: Supplementary Figure 1 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism
Supplementary Figure 1 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism Open
S1. Blocking O-GlcNAcylation in vivo by inducing shOGT or alloxan treatment qualitatively reduces EZH2 immunoreactivity after irradiation.
View article: Authorship Change Form from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism
Authorship Change Form from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism Open
S8. WTN, KON, WTH, and KOH HCT116 cell proliferation after 5 days in two O2 conditions,21% and 1% for cells knocked down for GTR3, GTR14 , and HIF2α
View article: Supplementary Figure 4 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism
Supplementary Figure 4 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism Open
S4. Hypoxic regulation of glucose transporters and creatine kinases validation by PCR and western blot analysis.
View article: Supplementary Figure 6 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism
Supplementary Figure 6 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism Open
S6. Phosphocreatine/Creatine ratio per cell in WTN, KON, WTH, and KOH.
View article: Data from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism
Data from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism Open
Hypoxia-inducible factor 1α is a key regulator of the hypoxia response in normal and cancer tissues. It is well recognized to regulate glycolysis and is a target for therapy. However, how tumor cells adapt to grow in the absence of HIF1α i…
View article: Supplementary Figure 5 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism
Supplementary Figure 5 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism Open
S5. Statistical significance for mRNA PCR data.
View article: Supplemetary Data from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism
Supplemetary Data from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism Open
Supplementary materials and methods
View article: Supplementary Figure 4 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism
Supplementary Figure 4 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism Open
S4. Hypoxic regulation of glucose transporters and creatine kinases validation by PCR and western blot analysis.
View article: Supplementary Figure 7 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism
Supplementary Figure 7 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism Open
S7. WTN, KON, WTH, and KOH HCT116 cell proliferation after 5 days in two O2 conditions, 21% and 1% for cells knocked down for GTR3, GTR14, and HIF2α.
View article: Supplementary Figure 3 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism
Supplementary Figure 3 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism Open
S3. HIF1α and hypoxia-dependent effects on transcription and protein levels.
View article: Data from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism
Data from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism Open
Hypoxia-inducible factor 1α is a key regulator of the hypoxia response in normal and cancer tissues. It is well recognized to regulate glycolysis and is a target for therapy. However, how tumor cells adapt to grow in the absence of HIF1α i…
View article: Supplementary Figure 6 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism
Supplementary Figure 6 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism Open
S6. Phosphocreatine/Creatine ratio per cell in WTN, KON, WTH, and KOH.
View article: Supplementary Figure 7 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism
Supplementary Figure 7 from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism Open
S7. WTN, KON, WTH, and KOH HCT116 cell proliferation after 5 days in two O2 conditions, 21% and 1% for cells knocked down for GTR3, GTR14, and HIF2α.
View article: Supplemetary Data from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism
Supplemetary Data from Adaptation to HIF1α Deletion in Hypoxic Cancer Cells by Upregulation of GLUT14 and Creatine Metabolism Open
Supplementary materials and methods