Cristina Pagani
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View article: Evaluation of 16S-Based Metagenomic NGS as Diagnostic Tool in Different Types of Culture-Negative Infections
Evaluation of 16S-Based Metagenomic NGS as Diagnostic Tool in Different Types of Culture-Negative Infections Open
Bacterial infections pose significant global health challenges, often underestimated due to difficulties in accurate diagnosis, especially when culture-based diagnostics fail. This study assesses the effectiveness of 16S-based metagenomic …
View article: Impact of a Bundle of Interventions on Quality-of-Care Indicators for Staphylococcus aureus Bacteraemia: A Single-Centre, Quasi-Experimental, Before–After Study
Impact of a Bundle of Interventions on Quality-of-Care Indicators for Staphylococcus aureus Bacteraemia: A Single-Centre, Quasi-Experimental, Before–After Study Open
Staphylococcus aureus bacteraemia (SAB) is a life-threatening bloodstream infection. Improved adherence to quality-of-care indicators (QCIs) can significantly enhance patient outcomes. This quasi-experimental study evaluated the impact of …
View article: First imported case of Candida auris infection in Milan, Italy: genomic characterisation
First imported case of Candida auris infection in Milan, Italy: genomic characterisation Open
Purpose Candida auris , an emerging multidrug-resistant yeast, has been reported worldwide. In Italy, the first case was reported in 2019. We describe the first case of C. auris , imported from Greece, in Milan, using whole genome sequenci…
View article: Cultivation and sequencing-free protocol for Serratia marcescens detection and typing
Cultivation and sequencing-free protocol for Serratia marcescens detection and typing Open
View article: Traditional Cultures versus Next Generation Sequencing for Suspected Orthopedic Infection: Experience Gained from a Reference Centre
Traditional Cultures versus Next Generation Sequencing for Suspected Orthopedic Infection: Experience Gained from a Reference Centre Open
(Background) The diagnosis and the antimicrobial treatment of orthopedic infection are challenging, especially in cases with culture-negative results. New molecular methods, such as next-generation sequencing (NGS), promise to overcome som…
View article: Bloodstream Infections in Intensive Care Unit during Four Consecutive SARS-CoV-2 Pandemic Waves
Bloodstream Infections in Intensive Care Unit during Four Consecutive SARS-CoV-2 Pandemic Waves Open
Critically ill COVID-19 patients are at an increased risk of bloodstream infections (BSIs). We performed a retrospective observational single-center study on COVID-19 patients admitted to intensive care unit (ICU) to assess the incidence o…
View article: Cultivation-free and sequencing-free protocol for the simultaneous detection and typing of Serratia marcescens: a rapid and cost-effective tool for large environmental/clinical screenings
Cultivation-free and sequencing-free protocol for the simultaneous detection and typing of Serratia marcescens: a rapid and cost-effective tool for large environmental/clinical screenings Open
Serratia marcescens is an opportunistic pathogen able to cause severe and lethal infections.The bacterium is able to survive in inhospitable environments (e.g.soap dispenser) and to rapidly spread among patients, causing large outbreaks, i…
View article: Risk factors associated with bacteremia in COVID-19 patients admitted to intensive care unit: a retrospective multicenter cohort study
Risk factors associated with bacteremia in COVID-19 patients admitted to intensive care unit: a retrospective multicenter cohort study Open
View article: Presence of carbepenemase-producing Enterobacteriaceae in the River Lambro basin, Italy: might sediment represent an important resistance reservoir?
Presence of carbepenemase-producing Enterobacteriaceae in the River Lambro basin, Italy: might sediment represent an important resistance reservoir? Open
In the last years, the rapid spread in anthropized ecosystems of pathogens which are resistant to carbapenem antibiotics has raised great concern. In this study, KPC-producing Klebsiella pneumoniae was found in the River Lambro in June 201…
View article: What was behind the first recognition and characterization of autochthonous SARS‐CoV‐2 transmission in Italy: The impact on European scenario
What was behind the first recognition and characterization of autochthonous SARS‐CoV‐2 transmission in Italy: The impact on European scenario Open
An Italian male with no link to China Severe Acute Respiratory Syndrome Coronavirus 2 (SARS‐CoV‐2) epidemic presented at Emergency Room (ER) with severe respiratory impairment. The RT‐PCR on 20 February 2020, nasopharyngeal swab revealed S…
View article: What was behind the first recognition and characterization of autochthonous SARS-CoV-2 transmission in Italy: who opened Pandora’s Box in Europe?
What was behind the first recognition and characterization of autochthonous SARS-CoV-2 transmission in Italy: who opened Pandora’s Box in Europe? Open
An Italian male with no link to China SARS-CoV-2 epidemic presented at Emergency Room with severe respiratory impairment. The RT-PCR on 20th February, 2020, nasopharyngeal swab revealed SARS-CoV-2 infection, confirmed with viral culture an…
View article: Geographical reconstruction of the SARS‐CoV‐2 outbreak in Lombardy (Italy) during the early phase
Geographical reconstruction of the SARS‐CoV‐2 outbreak in Lombardy (Italy) during the early phase Open
The first identification of autochthonous transmission of SARS‐CoV‐2 in Italy was documented by the Laboratory of Clinical Microbiology, Virology and Bioemergencies of L. Sacco Hospital (Milano, Italy) on 20th February 2020 in a 38 years o…
View article: Presence and infectivity of SARS-CoV-2 virus in wastewaters and rivers
Presence and infectivity of SARS-CoV-2 virus in wastewaters and rivers Open
View article: Presence and vitality of SARS-CoV-2 virus in wastewaters and rivers
Presence and vitality of SARS-CoV-2 virus in wastewaters and rivers Open
Wastewater-based epidemiology has been proposed to monitor the diffusion and trend of SARS-CoV-2 pandemic. In the present study, raw and treated samples from three wastewater treatment plants, and two river samples characterized the Milano…
View article: Tocilizumab for cytokine storm syndrome in COVID-19 pneumonia: an increased risk for candidemia?
Tocilizumab for cytokine storm syndrome in COVID-19 pneumonia: an increased risk for candidemia? Open
View article: Microbial biofilm correlates with an increased antibiotic tolerance and poor therapeutic outcome in infective endocarditis
Microbial biofilm correlates with an increased antibiotic tolerance and poor therapeutic outcome in infective endocarditis Open
View article: The Role of <i>Staphylococcus aureus</i> in Mastitis
The Role of <i>Staphylococcus aureus</i> in Mastitis Open
Background Breastfeeding women are at risk of developing mastitis during the lactation period. Staphylococcus aureus has emerged as the community-acquired pathogen responsible for virulence (methicillin resistance and Panton-Valentine leuk…
View article: Mingling of human and veterinary strains of Staphylococcus aureus: An emerging issue in health-care systems
Mingling of human and veterinary strains of Staphylococcus aureus: An emerging issue in health-care systems Open
Aim: Methicillin-resistant Staphylococcus aureus remains a leading cause of hospital and community infections. We report a retrospective molecular characterization of S. aureus strains from different settings: hospital workers and patients…
View article: Whole genome sequencing for the molecular characterization of carbapenem-resistant Klebsiella pneumoniae strains isolated at the Italian ASST Fatebenefratelli Sacco Hospital, 2012–2014
Whole genome sequencing for the molecular characterization of carbapenem-resistant Klebsiella pneumoniae strains isolated at the Italian ASST Fatebenefratelli Sacco Hospital, 2012–2014 Open
Our findings support the importance of implementing bacterial genomics in clinical medicine in order to complement traditional methods and overcome their limited resolution.
View article: Remitting infections due to community-acquired Panton–Valentine leukocidin-producing Staphylococcus aureus in the Milan area
Remitting infections due to community-acquired Panton–Valentine leukocidin-producing Staphylococcus aureus in the Milan area Open
One of the most important Staphylococcus aureus virulence factors is Panton-Valentine leukocidin (PVL). We describe an outbreak of recurrent cutaneous PVL infections in different members of three family clusters. Molecular investigations w…
View article: Additional file 1: Table S1. of Whole genome sequencing for the molecular characterization of carbapenem-resistant Klebsiella pneumoniae strains isolated at the Italian ASST Fatebenefratelli Sacco Hospital, 2012â 2014
Additional file 1: Table S1. of Whole genome sequencing for the molecular characterization of carbapenem-resistant Klebsiella pneumoniae strains isolated at the Italian ASST Fatebenefratelli Sacco Hospital, 2012â 2014 Open
MICs (Îźg/ml) of antimicrobial agents for carbapenem-resistant K. pneumoniae strains tested in this study. (XLS 31Â kb)
View article: Additional file 4: Figure S2. of Whole genome sequencing for the molecular characterization of carbapenem-resistant Klebsiella pneumoniae strains isolated at the Italian ASST Fatebenefratelli Sacco Hospital, 2012â 2014
Additional file 4: Figure S2. of Whole genome sequencing for the molecular characterization of carbapenem-resistant Klebsiella pneumoniae strains isolated at the Italian ASST Fatebenefratelli Sacco Hospital, 2012â 2014 Open
Genome analysis of the Yersinia pestis high pathogenicity island in the 68 carbapenem-resistant K. pneumoniae strains . The Mauve multiple alignment between the Kp13 K. pneumoniae strain (GenBank accession No. CP003999) and our isolates ev…
View article: Can Drainage Using a Negative-Pressure Wound Therapy Device Replace Traditional Sample Collection Methods?
Can Drainage Using a Negative-Pressure Wound Therapy Device Replace Traditional Sample Collection Methods? Open
As a result of proficiency gained in the last year, the most representative specimen in wound infection was represented by mediastinal fluid collected directly from the wound and the NPWT device. Given the correlation between the blood cul…
View article: Pasteurella spp in Knee Mega Prosthesis Infection: A Case Report
Pasteurella spp in Knee Mega Prosthesis Infection: A Case Report Open
Pasteurella spp is the most frequently pathogen isolated from infected wound inflicted by domestic animal bites. Here we present a case of isolation of Pasteurella pneumotropica like organism from knee mega prosthesis infection occurred in…
View article: Role of<i>Chryseobacterium indologenes</i>Infection in a Female with Mediastinitis
Role of<i>Chryseobacterium indologenes</i>Infection in a Female with Mediastinitis Open
Background:Chryseobacterium indologenes is an uncommon human pathogen that has been rarely reported in the literature as a cause of infection, but despite low virulence, this opportunistic bacteria is resistant to many antimicrobial agents…
View article: Diffusion and transmission of carbapenem-resistant Klebsiella pneumoniae in the medical and surgical wards of a university hospital in Milan, Italy
Diffusion and transmission of carbapenem-resistant Klebsiella pneumoniae in the medical and surgical wards of a university hospital in Milan, Italy Open