Daniel Angel‐Velez
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Lipopolysaccharide reduces progesterone and cytokines in equine follicular fluid without affecting oocyte development in vitro Open
Lipopolysaccharide (LPS) in follicular fluid impairs steroid production and oocyte developmental competence in cows and mice. This study assessed LPS concentrations in equine follicular fluid and their association with steroid and some cyt…
Biphasic CAPA-IVM Improves Equine Oocyte Quality and Subsequent Embryo Development Without Inducing Genetic Aberrations Open
In vitro maturation (IVM) of oocytes retrieved from ovum pick-up (OPU) or ovarian tissue (OT) is a standard approach for patients with specific conditions where prior hormonal stimulation is contraindicated. However, the developmental comp…
Biphasic CAPA-IVM Improves Equine Oocyte Quality and Subsequent Embryo Development Without Inducing Genetic Aberrations Open
In vitro maturation (IVM) of oocytes retrieved from ovum pick-up (OPU) or ovarian tissue (OT) is a standard approach for patients with specific conditions where prior hormonal stimulation is contraindicated. However, the developmental comp…
Origin and development of uniparental and polyploid blastomeres Open
Whole-genome (WG) abnormalities, such as uniparental diploidy and triploidy, cause fetal death. Occasionally, they coexist with biparental diploid cells in live births. Understanding the origin and early development of WG abnormal blastome…
Decoding bull fertility in vitro: a proteomics exploration from sperm to blastocyst Open
In brief Bulls are selected for field fertility and semen quality, but traits such as polyspermy are not considered and can increase aneuploidy during in vitro embryo production. This study links bull-specific proteomic signatures to polys…
Origin and development of uniparental and polyploid blastomeres Open
Whole-genome (WG) abnormalities, such as uniparental diploidy and triploidy, cause fetal death. Occasionally, they coexist with biparental diploid cells in live births. Understanding the origin and early development of WG abnormal blastome…
Single closed-tube quantitative real-time PCR assay with dual-labelled probes for improved sex determination of equine embryos Open
In addition to fulfilling many breeders' curiosity, equine embryonic sex determination can have a profound commercial impact. However, the application of currently described assays for equine embryonic sexing has rendered variable diagnosi…
Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates Open
In vivo-matured oocytes exhibit higher developmental competence than those matured in vitro but mimicking the in vivo environment by in vitro conditions has been challenging. Until now, conventional two-dimensional (2D) systems have been u…
Transcriptomics Reveal Molecular Differences in Equine Oocytes Vitrified before and after In Vitro Maturation Open
In the last decade, in vitro embryo production in horses has become an established clinical practice, but blastocyst rates from vitrified equine oocytes remain low. Cryopreservation impairs the oocyte developmental potential, which may be …
View article: Measuring cumulus expansion of mammalian oocytes: comparing the reliability of methods and how artificial intelligence could automate the measurement
Measuring cumulus expansion of mammalian oocytes: comparing the reliability of methods and how artificial intelligence could automate the measurement Open
Cumulus expansion is an important indicator of oocyte maturation, often correlated with greater oocyte developmental capacity. Although multiple methods have been described to assess cumulus expansion, none of them is considered a gold sta…
Alternative culture systems for bovine oocyte <i>in vitro</i> maturation: liquid marbles and differentially shaped 96-well plates Open
In vivo matured oocytes exhibit higher developmental competence than those matured in vitro , but mimicking the in vivo environment by in vitro conditions has been challenging. Till now, conventional two-dimensional (2D) systems have been …
Detecting the Area of Bovine Cumulus Oocyte Complexes Using Deep Learning and Semantic Segmentation Open
The cumulus-oocyte complex (COC) is an oocyte surrounded by specialized granulosa cells, called cumulus cells. The cumulus cells surrounding the oocyte ensure healthy oocyte and embryo development. The maturity of COCs at oocyte retrieval …
View article: Lycopene supplementation to serum‐free embryo culture medium and its effect on development and quality of bovine blastocysts produced in vitro
Lycopene supplementation to serum‐free embryo culture medium and its effect on development and quality of bovine blastocysts produced in vitro Open
Selenium is commonly used as an antioxidant in a serum‐free culture medium setting. However, lycopene has emerged as a potent antioxidant being twice as efficient as β‐carotene and 10 times as efficient as α‐tocopherol with beneficial effe…
View article: Lycopene Supplementation to Serum-Free Maturation Medium Improves In Vitro Bovine Embryo Development and Quality and Modulates Embryonic Transcriptomic Profile
Lycopene Supplementation to Serum-Free Maturation Medium Improves In Vitro Bovine Embryo Development and Quality and Modulates Embryonic Transcriptomic Profile Open
Bovine embryos are typically cultured at reduced oxygen tension to lower the impact of oxidative stress on embryo development. However, oocyte in vitro maturation (IVM) is performed at atmospheric oxygen tension since low oxygen during mat…
New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification Open
Equine oocyte vitrification would benefit the growing in vitro embryo production programs, but further optimization of the protocol is necessary to reach clinical efficiency. Therefore, we aimed to perform a direct comparison of non-permea…
Anti-Müllerian Hormone and OPU-ICSI Outcome in the Mare Open
Anti-Müllerian hormone (AMH) reflects the population of growing follicles and has been related to mammalian fertility. In the horse, clinical application of ovum pick-up and intracytoplasmic sperm injection (OPU-ICSI) is increasing, but re…