D. Barrie Johnson
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View article: Acidithiobacillia class members originating at sites within the Pacific Ring of Fire and other tectonically active locations and description of the novel genus ‘Igneacidithiobacillus’
Acidithiobacillia class members originating at sites within the Pacific Ring of Fire and other tectonically active locations and description of the novel genus ‘Igneacidithiobacillus’ Open
Recent studies have expanded the genomic contours of the Acidithiobacillia , highlighting important lacunae in our comprehension of the phylogenetic space occupied by certain lineages of the class. One such lineage is ‘ Igneacidithiobacill…
View article: Influence of mobile genetic elements and insertion sequences in long- and short-term adaptive processes of Acidithiobacillus ferrooxidans strains
Influence of mobile genetic elements and insertion sequences in long- and short-term adaptive processes of Acidithiobacillus ferrooxidans strains Open
The recent revision of the Acidithiobacillia class using genomic taxonomy methods has shown that, in addition to the existence of previously unrecognized genera and species, some species of the class harbor levels of divergence that are co…
View article: Implementation of genomic surveillance of SARS-CoV-2 in the Caribbean: Lessons learned for sustainability in resource-limited settings
Implementation of genomic surveillance of SARS-CoV-2 in the Caribbean: Lessons learned for sustainability in resource-limited settings Open
The COVID-19 pandemic highlighted the importance of global genomic surveillance to monitor the emergence and spread of SARS-CoV-2 variants and inform public health decision-making. Until December 2020 there was minimal capacity for viral g…
View article: Comparison of different small molecular weight alcohols for sustaining sulfidogenic bioreactors maintained at moderately low pH
Comparison of different small molecular weight alcohols for sustaining sulfidogenic bioreactors maintained at moderately low pH Open
Sulfate-reducing bacteria (SRB) catalyse the dissimilatory reduction of sulfate to hydrogen sulfide using a wide range of small molecular weight organic compounds, and hydrogen, as electron donors. Here we report the effects of different c…
View article: Draft Genome Sequence of <i>Firmicutes</i> Strain S <sup>0</sup> AB, a Heterotrophic Iron/Sulfur-Oxidizing Extreme Acidophile
Draft Genome Sequence of <i>Firmicutes</i> Strain S <sup>0</sup> AB, a Heterotrophic Iron/Sulfur-Oxidizing Extreme Acidophile Open
The draft whole-genome sequence of the extremely acidophilic and novel Firmicutes strain S 0 AB is reported. The genome comprises 3.3 Mbp and has a GC content of 43.72%. In total, 3,240 protein-coding genes, 56 tRNA genes, and 11 rRNA gene…
View article: Draft Genome Sequence of the Novel, Moderately Thermophilic, Iron- and Sulfur-Oxidizing Firmicute Strain Y002, Isolated from an Extremely Acidic Geothermal Environment
Draft Genome Sequence of the Novel, Moderately Thermophilic, Iron- and Sulfur-Oxidizing Firmicute Strain Y002, Isolated from an Extremely Acidic Geothermal Environment Open
We report the draft genome sequence of the Firmicute strain Y002, a facultatively anaerobic, acidophilic bacterium that catalyzes the dissimilatory oxidation of iron and sulfur and the reduction of ferric iron. Analysis of the genome (2.9 …
View article: Chromium (VI) Inhibition of Low pH Bioleaching of Limonitic Nickel-Cobalt Ore
Chromium (VI) Inhibition of Low pH Bioleaching of Limonitic Nickel-Cobalt Ore Open
Limonitic layers of the regolith, which are often stockpiled as waste materials at laterite mines, commonly contain significant concentrations of valuable base metals, such as nickel, cobalt, and manganese. There is currently considerable …
View article: Bioleaching of Transition Metals From Limonitic Laterite Deposits and Reassessment of the Multiple Roles of Sulfur-Oxidizing Acidophiles in the Process
Bioleaching of Transition Metals From Limonitic Laterite Deposits and Reassessment of the Multiple Roles of Sulfur-Oxidizing Acidophiles in the Process Open
Using acidophilic bacteria to catalyze the reductive dissolution of oxidized minerals is an innovative process that facilitates the extraction of valuable base metals (principally cobalt and nickel) from limonites, which are otherwise ofte…
View article: Genomic evolution of the class <i>Acidithiobacillia</i>: deep-branching Proteobacteria living in extreme acidic conditions
Genomic evolution of the class <i>Acidithiobacillia</i>: deep-branching Proteobacteria living in extreme acidic conditions Open
Members of the genus Acidithiobacillus, now ranked within the class Acidithiobacillia, are model bacteria for the study of chemolithotrophic energy conversion under extreme conditions. Knowledge of the genomic and taxonomic diversity of Ac…
View article: Design and Operation of Empirical Manganese-Removing Bioreactors and Integration into a Composite Modular System for Remediating and Recovering Metals from Acidic Mine Waters
Design and Operation of Empirical Manganese-Removing Bioreactors and Integration into a Composite Modular System for Remediating and Recovering Metals from Acidic Mine Waters Open
Packed bed bioreactors were used to remove soluble manganese from a synthetic mine water as the final stage of an integrated bioremediation process. The synthetic mine water had undergone initial processing using a sulfidogenic bioreactor …
View article: Dissolution of Manganese (IV) Oxide Mediated by Acidophilic Bacteria, and Demonstration That Manganese (IV) Can Act as Both a Direct and Indirect Electron Acceptor for Iron-Reducing <i>Acidithiobacillus</i> spp.
Dissolution of Manganese (IV) Oxide Mediated by Acidophilic Bacteria, and Demonstration That Manganese (IV) Can Act as Both a Direct and Indirect Electron Acceptor for Iron-Reducing <i>Acidithiobacillus</i> spp. Open
Experiments were carried out to examine the oxido-reduction of manganese by extremely acidophilic Acidithiobacillus spp. grown with either elemental sulfur or molecular hydrogen as electron donor. While there was no evidence for manganese …
View article: A Model of Aerobic and Anaerobic Metabolism of Hydrogen in the Extremophile Acidithiobacillus ferrooxidans
A Model of Aerobic and Anaerobic Metabolism of Hydrogen in the Extremophile Acidithiobacillus ferrooxidans Open
Hydrogen can serve as an electron donor for chemolithotrophic acidophiles, especially in the deep terrestrial subsurface and geothermal ecosystems. Nevertheless, the current knowledge of hydrogen utilization by mesophilic acidophiles is mi…
View article: Genome-based classification of Acidihalobacter prosperus F5 (=DSM 105917=JCM 32255) as Acidihalobacter yilgarnensis sp. nov.
Genome-based classification of Acidihalobacter prosperus F5 (=DSM 105917=JCM 32255) as Acidihalobacter yilgarnensis sp. nov. Open
The genus Acidihalobacter has three validated species, Acidihalobacter ferrooxydans , Acidihalobacter prosperus and Acidihalobacter aeolinanus , all of which were isolated from Vulcano island, Italy. They are obligately chemolithotrophic, …
View article: Characteristics of an Iron-Reducing, Moderately Acidophilic Actinobacterium Isolated from Pyritic Mine Waste, and Its Potential Role in Mitigating Mineral Dissolution in Mineral Tailings Deposits
Characteristics of an Iron-Reducing, Moderately Acidophilic Actinobacterium Isolated from Pyritic Mine Waste, and Its Potential Role in Mitigating Mineral Dissolution in Mineral Tailings Deposits Open
Reactive pyritic mine tailings can be populated by chemolithotrophic prokaryotes that enhance the solubilities of many metals, though iron-reducing heterotrophic microorganisms can inhibit the environmental risk posed by tailings by promot…
View article: Bioleaching of arsenic-rich cobalt mineral resources, and evidence for concurrent biomineralisation of scorodite during oxidative bio-processing of skutterudite
Bioleaching of arsenic-rich cobalt mineral resources, and evidence for concurrent biomineralisation of scorodite during oxidative bio-processing of skutterudite Open
Experiments were carried out to test the amenabilities of mineral deposits that contained cobalt deported in arseno-sulfide (cobaltite) and arsenide (skutterudite) minerals, to oxidative bioleaching at mesophilic temperatures and low pH. A…
View article: Removal of Zinc From Circum-Neutral pH Mine-Impacted Waters Using a Novel “Hybrid” Low pH Sulfidogenic Bioreactor
Removal of Zinc From Circum-Neutral pH Mine-Impacted Waters Using a Novel “Hybrid” Low pH Sulfidogenic Bioreactor Open
Environmental pollution associated with metal-contaminated waters discharging from abandoned mine sites is a global issue. Remediation using passive systems, such as constructed wetlands, has several significant detractions which active tr…
View article: Bacterial and Archaeal Diversity in Sulfide-Bearing Waste Rock at Faro Mine Complex, Yukon Territory, Canada
Bacterial and Archaeal Diversity in Sulfide-Bearing Waste Rock at Faro Mine Complex, Yukon Territory, Canada Open
Acid mine/rock drainage (AMD/ARD) is generated by the microbially-accelerated oxidative dissolution of sulfide minerals in working and abandoned mine sites, and some natural environments. Iron-oxidizing microorganisms (IOM) regenerate the …
View article: Acidithiobacillus ferrianus sp. nov.: an ancestral extremely acidophilic and facultatively anaerobic chemolithoautotroph
Acidithiobacillus ferrianus sp. nov.: an ancestral extremely acidophilic and facultatively anaerobic chemolithoautotroph Open
Strain MG, isolated from an acidic pond sediment on the island of Milos (Greece), is proposed as a novel species of ferrous iron- and sulfur-oxidizing Acidithiobacillus . Currently, four of the eight validated species of this genus oxidize…
View article: Acidophile Microbiology in Space and Time
Acidophile Microbiology in Space and Time Open
The study of extreme acidophiles, broadly defined as microorganisms that grow optimally at pH values below 3, was initiated by the discovery by Waksman and Joffe in the early 1900s of a bacterium that was able to live in the dilute sulfuri…
View article: Biological removal of sulfurous compounds and metals from inorganic wastewaters
Biological removal of sulfurous compounds and metals from inorganic wastewaters Open
This second edition is fully updated with new material to create a comprehensive and accessible reference book: New chapters on sulfur removal via bioelectrochemical systems, use of sulfate radicals in advanced oxidation processes and sulf…
View article: Upconversion of Cellulosic Waste Into a Potential “Drop in Fuel” via Novel Catalyst Generated Using Desulfovibrio desulfuricans and a Consortium of Acidophilic Sulfidogens
Upconversion of Cellulosic Waste Into a Potential “Drop in Fuel” via Novel Catalyst Generated Using Desulfovibrio desulfuricans and a Consortium of Acidophilic Sulfidogens Open
Biogas-energy is marginally profitable against the "parasitic" energy demands of processing biomass. Biogas involves microbial fermentation of feedstock hydrolyzate generated enzymatically or thermochemically. The latter also produces 5-hy…
View article: Bioreductive Dissolution as a Pretreatment for Recalcitrant Rare-Earth Phosphate Minerals Associated with Lateritic Ores
Bioreductive Dissolution as a Pretreatment for Recalcitrant Rare-Earth Phosphate Minerals Associated with Lateritic Ores Open
Recent research has demonstrated the applicability of a biotechnological approach for extracting base metals using acidophilic bacteria that catalyze the reductive dissolution of ferric iron oxides from oxidized ores, using elemental sulfu…
View article: Design and Application of a Low pH Upflow Biofilm Sulfidogenic Bioreactor for Recovering Transition Metals From Synthetic Waste Water at a Brazilian Copper Mine
Design and Application of a Low pH Upflow Biofilm Sulfidogenic Bioreactor for Recovering Transition Metals From Synthetic Waste Water at a Brazilian Copper Mine Open
A sulfidogenic bioreactor, operated at low pH (4-5), was set up and used to remove transition metals (copper, nickel, cobalt, and zinc) from a synthetic mine water, based on the chemistry of a moderately acidic (pH 5) drainage stream at an…